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Biomedical subjects

O A Dada

Publications and source records attributed to O A Dada.

At least 37 records · Page 2Linked to original sources
PubMed1982

The effect of alcohol on the in vitro absorption of estradiol and estrone sulphate in rat intestine.

The intestinal absorption of [3H]-estradiol and [3H]-estrone sulphate was studied by measuring the rate of transport of these estrogens from the mucosal to the serosal side of isolated sacs of rat duodenum. Ethanol, at concentrations of 40 mM or higher, significantly enhanced estradiol while inhibiting estrone sulphate transport. Relatively less estradiol was transported as estrone glucuronide and more as free estrone in the presence of 400 mM ethanol than in control sacs. In contrast, 400 mM ethanol resulted in relatively more estrone sulphate being transported unchanged than in the control.

Animals↗
PubMed1982

Effect of chloroquine administration on the in vitro conjugation of oestradiol-17 beta by rat liver.

The conjugation of oestradiol-17 beta was studied in control and chloroquine-treated rats. Liver homogenates were incubated with [14C]oestradiol-17 beta and cofactors for 4 h at 37 degrees C and free radioactivity was extracted before and after hot acid hydrolysis. The extent of conjugation was estimated from the ratio of free radioactivity to free plus conjugated radioactivity. The method was authenticated by the negligibly low conjugation obtained for either heat-denatured homogenates or water blank. Each experiment was corrected for its own recovery. The effects of both single and multiple intramuscular injections of chloroquine phosphate to rats on the in vitro conjugation of oestradiol-17 beta were studied separately. It was observed that both procedures significantly potentiated conjugation.

Animals↗
PubMed1980

Endocrine function and haemoglobinopathies: relation between the sickle cell gene and circulating plasma levels of testosterone, luteinising hormone (LH) and follicle stimulating hormone (FSH) in adult males.

Circulating plasma levels of testosterone, luteinising hormone and follicle stimulating hormone were determined in adult Nigerian males who have haemoglobin (Hb) genotypes HbAA, AS, AC, SC and SS. The levels of the three hormones were significantly lower in homozygous sickle cell disease (HbSS) compared to the other groups, whereas the levels of testosterone and luteinising hormone were significantly reduced in HbSC heterozygotes compared to HbAS heterozygotes only. These results are suggestive of an association of secondary hypogonadism with haemoglobin S disease.

Adult↗
PubMed1980

Prognosis of pregnancy after threatened abortion.

Uterine bleeding in early pregnancy represents a definite threat to the developing embryo and constitutes a source of anxiety to both the patient and her clinician. In a prospective study of women with threatened abortion (TA) in the first and second trimesters of pregnancy in Ibadan, Nigeria, 48.8% of them aborted their pregnancies and there was no significant association between the various sociomedical factors considered and the outcome of pregnancy after TA. In like manner, routine admission of patients with TA into the hospital may not be essential for the preservation of the pregnancy. However, uterine bleeding as a symptom of abortion appears to be of greater risk if this occurs in the second trimester of pregnancy, and the predictive value of circulating progesterone levels in TA is significant. It is probable that uterine bleeding in the second trimester, especially when this is associated with low circulating levels of progesterone, may be pathognomonic of imminent abortion.

Abortion, Threatened↗
PubMed1980

Isopycnic centrifugation of human, pigeon and guinea pig erythrocytes.

The buoyant densities of human erythrocytes with normal (HbAA) and abnormal heterozygous haemoglobins (HbSS, HbAS, HbSC) as well as erythrocytes from guinea pig and pigeon were determined by centrifugation in discontinuous density gradients of metrizamide. Guinea pig, pigeon and human erythrocytes from normal human adult (HbAA) and from heterozygote HbAS were found to have the same density of 1.1381 g/cm3, while erythrocytes from a sickle-cell patient (HbS) and from a heterozygote HbSC, HbSS and HbSC, which are known to have a higher lipid content than isovolumic normal cells, had a lower density of 1.1261 g/cm3. When centrifugation was carried out under non-equilibrium conditions (rate zonal), several sedimentation bands were obtained from each erythrocyte preparation. Differences in the sedimentation rates were unrelated to the haemoglobin composition of the erythrocytes. It is suggested that the lower density of the erythrocytes associated with sickle-cell disease may have significant consequences in the circulation of such cells and in the clinical sequence of the sickling process.

Anemia, Sickle Cell↗
PubMed1978

Effect of graded oral doses of oestradiol on circulating hormonal levels.

A single oral dose of micronized oestradiol (1, 2 or 4 mg) was administered to 10 normally menstruating women on the second day of 3 consecutive menstrual cycles. The order in which the 3 doses were given was chosen randomly for each subject. Samples of peripheral blood were withdrawn on 3 occasions (10, 5 and 1 min) before and on 9 occasions (0.37, 0.75, 1.5, 30, 6.0, 12.0, 24.0, 48.0 and 72.0 h) after the ingestion of oestradiol. Oestradiol, oesterone, oestradiol sulphate, oestrone sulphate, testosterone and LH were measured by means of radioimmunoassays in blood plasma on all occasions. The levels of all oestrogens increased significantly following the ingestion of oestradiol, although to a greatly different extent. The largest and most prolonged increase was seen in the case of oestrone sulphate, the levels of which--depending on the dose ingested--were 20-50 times higher than the pre-treatment levels 3 after the ingestion of the oestradiol capsule. The increase of the levels of oestrone sulphate was still significant (P less than 0.001) after 24 h, and following the ingestion of 2 and 4 mg of oestradiol even after 72 h (P less than 0.05). The levels of oestrone and oestradiol sulphate exhibited smaller increases 3 h after ingestion, i.e. 4-10 and 2.5-5 times, respectively, and the plasma levels of oestradiol showed a minor increase only (1.4-1.8 times). The concentration of oestradiol metabolites studied increased proportionally with the dose of oestradiol administered. On the other hand, a clear-cut dose--effect relationship could not be observed between the amount of oestradiol administered and the plasma levels of LH and testosterone. The data indicate that the bulk of perorally administered oestradiol is transformed to oestrone sulphate accompanied by smaller quantities of oestrone and oestradiol sulphate and that the ratios of circulating oestrogens seen after the ingestion of oestradiol deviate considerably from those observed in the normal menstrual cycle.

Administration, Oral↗
PubMed1977

Contraception and haemoglobinopathies in Ibadan, Nigeria. An evaluation of the effect on anaemia.

The haemoglobin-genotype of women attending six family planning clinics in the city of Ibadan, Nigeria in a 16-month period was determined. Women with haemoglobinopathy S and C (Hb SS, SC and AC), in spite of the greater risks associated with pregnancy in them, were found to use the services of the family planning clinics less than others (Hb AA and AS) in the population. Women with Hb AA or Hb AS on different contraceptives had similar levels of erythrocyte packed cell volume except that reduced levels were observed in Hb AS acceptors on oral contraceptives not containing an iron supplement.

Adolescent↗
PubMed1976

The effect of intra-uterine contraceptive devices on the oestrogen-induced incorporation of [3H-5]uridine into RNA by the rat uterus.

A length of copper or stainless steel wire or of silk thread suture was inserted as an intra-uterine device (IUD) for 3 days in the right uterine horn of ovariectomized virgin female rats. The left horn served as a control. The control rats were ovariectomized but had no IUD inserted. The in vitro incorporation of [3H-5]uridine into uterine RNA was significantly inhibited (P less than 0.005) in copper-bearing horns compared to control horns, while silk and steel devices had no inhibitory effect, thus indicating an effect of intra-uterine copper on oestradiol-induced uterine metabolism. The insertion of an intra-uterine device had no significant effect on uterine wet weight, DNA content or on the uptake of [3H-5]uridine into the nucleotide pool.

Animals↗
PubMed1975

Total urinary oestrogen excretion during normal menstrual cycle and normal pregnancy in Nigerians.

Total oestrogens were measured during normal menstrual cycles and during pregnancy in healthy Nigerian women. During the menstrual cycle, maximum excretion was consistently observed in the luteal phase in contrast to findings in European and American populations. During pregnancy, oestrogen excretion was similar to that reported amongst Europeans only in early pregnancy; oestrogen excretion did not show the usual rapid rise in late pregnancy.

Adolescent↗
PubMedDate not supplied

Inhibition of gonadotropin and prostaglandin stimulation of testicular steroidogenesis in malnourished rats.

The effect of human chorionic gonadotropin (hCG) and prostaglandin E1 (PGE1) on testicular steroidogenesis in protein-deficient and refed rats was studied in vitro. The malnourished, refed, and control rats were found to secret testosterone in response to hCG and PGE1 stimulation. There was a significant reduction in the basal level of secretion in the malnourished rat testis (1.0 +/- 0.4 nMol/3 hr./Testis). Malnourished rats refed with adequate protein diet responded to hCG and PGE1 stimulation in a similar manner to normally-fed adult rats.

Alprostadil↗