[Free and bound iron in normal and tumor cells].
Explore the source record for details and available documents.
Animals↗
Biomedical subjects
O A Kovalenko
Publications and source records attributed to O A Kovalenko.
[Free iron in intracellular membrane structures].
Possibility of long-term existence of closed model membrane structures loaded with low molecular compounds of bivalent ferrum was shown. Liposomes prepared from phospholipids of egg yolk were used as a model. There were found similar effects of heating and detergents on the formation of nitrosyl complexes of non-heme iron (complexes 2.03) in the model system--liposomes loaded with ferrum on the one hand and mitochondria, hepatocytes and liver on the other. Heating and detergents intensify the formation of complexes 2.03, which is conditioned by iron liberation from the liposomes and closed intracellular membrane structures as a result of their destruction. A conclusion is drawn that free iron in animal liver cells is localized in the close membrane structures.
Animals↗
[Ratio between free and deposited iron in animal tissues].
When mice were given water diet with 57Fe a practically complete exchange in the blood 56Fe and 57Fe was observed during 2 months. 57Fe isotope was accumulated in iron storage of the liver, kidney and spleen. Partial isotope exchange for free iron occurred in these tissues too. The free iron content was not changed. This isotope exchange for free iron did not occur in tissues which were unable to accumulate 57Fe in iron storage.
Animals↗
[A quantitative description of the cytostatic effects of chelators of transition metal ions (exemplified by picolinic acid and Fe2+)].
A mathematical model is proposed for the description of the cytostatic effects of the chelating agents of the transition metal ions on cell cultures. Chelate complexes of picolinic acid and bivalent iron ions, which play an important role in nucleic acid synthesis, were considered as an example. Kinetic equations of the theory of receptors were used for model construction. The best correspondence with the experimental dose-effect curves, obtained in the experiments on influence of various picolinic acid concentrations on division of the SPEV cultured cells, is provided by the model of threshold type. The parameters lying in the basis of the theory of receptors and relating the biological effect (cell division) to the number of ligand-receptor complexes were calculated.
Animals↗