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Biomedical subjects

O Burducea

Publications and source records attributed to O Burducea.

29 records · Page 2Linked to original sources
PubMedDate not supplied

Preliminary data on the encapsulation of biologically active materials in liposomes.

Multilamellar and unilamellar phospholipid liposomes were prepared and investigated as regards their properties and the capacity of encapsulating biologically active materials, such as CrO4-(2-)ions, basic dyes, proteins, DNA, as well as Sendai virus particles. The efficiency of encapsulation ranged from 10% for CrO4-(2-)ions to about 80% for toluidine blue; it was found to depend not only on the type of encapsulated material, but also on the method used for liposome preparation and on liposome composition.

Animals↗
PubMedDate not supplied

Investigations of the antiviral activity of some nitroso-urea derivatives. Inhibitory action of the IOB-252 nitroso-urea derivative on vaccinia virus incell cultures.

The nitroso-urea derivative IOB-252 was administrered in monkey kidney cell cultures in a concentration of 40 mug/ml 24 hours before inoculation of vaccinia virus and maintained afterwards in a concentration of 25 mug/ml. The drug inhibited vaccinia virus multiplication, hemagglutinin synthesis and late cytopathic effect, but did not prevent early cytopathic lesions. IOB-252 inhibited the synthesis of interferon initiated by a viral inductor and blocked the antiviral effect of an exogeneous interferon. The mechanism of action of the drug is discussed.

Animals↗
PubMedDate not supplied

Stimulation by inlfuenza virus RNA of 3H-phenylalanine incorporation in a cell-free system.

Purified and unpurified cell-free systems prepared from the chorioallantoic membrane of embryonated eggs were tested for polypeptide synthesis in the presence and absence of influenza virus RNA. Both systems exhibited an endogenous messenger activity determining 3H-phenylalanine incorporation into polypeptides in the absence of virus RNA. However, addition of influenza virus RNA to the systems clearly stimulated amino acid incorporation into polypeptides, offering the possibility of studying some aspects of the viral protein biosynthesis mechanism.

Amino Acids↗
PubMedDate not supplied

Polypeptide changes in Sendai virus-infected cells.

The appearance of virus-specific proteins in Sendai virus-infected chick embryo fibroblasts and chorioallantoic membrane cells was studied by high resolution SDS-polyacrylamide gel electrophoresis. All the structural Sendai virus polypeptides, as well as the nonstructural virus polypeptide termed B could be identified in the total lysates of infected cells. Only the structural virus polypeptides NP, P and M were found in the ribosome fraction of virus-infected cells; these additional polypeptides were removed from the ribosome surface by washing the 1 M NH4Cl.

Animals↗
PubMedDate not supplied

Effect of biologically active compounds (anthracyclines and ethidium bromide) on some membrane-mediated processes in the course of viral infection. Investigations on a prokaryotic (bacteriophage-bacterium) system.

Anthracycline antibiotics--violamycin B1 and adriamycin--have an obvious effect on the efficiency of phage lambda L47.1 DNA transfection into E. coli Q358 cells. Treatment with anthracyclines of either phage DNA or bacterial cells results in a marked decrease in the number of transfectants per microgram DNA. On the other hand, adsorption of phage lambda gt WES to E. coli LE392 is considerably modified by exposure to anthracyclines of either the phage or the host cells.

Adsorption↗
PubMedDate not supplied

Investigations concerning the action of serveral chemical and biological agents on HBsAg.

Native and purified HBsAg preparations were subjected in vitro to the action of cetylpyridinium bromide (Bromocet), hibitan-chlorhexidine (Hibiscrub), chloramine B and propolis extract, at different concentrations and for various time intervals. The effect of these agents on the serological reactivity of HBsAg was tested by electroimmunodiffusion (EID) and radioimmunoassay (RIA). Chloramine B and the propolis extract had a significant inhibitory effect-ascertained by both EID and RIA - on purified HBsAg, but not on the native preparation. The inhibition exerted by Bromocet and Hibiscrub indicated by EID results was not confirmed by RIA.

Cetylpyridinium↗