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O S Zakharova

Publications and source records attributed to O S Zakharova.

5 recordsLinked to original sources
PubMed1981

[Model system for studying thrombocyte interaction with the reconstructed vascular wall].

A new model system is suggested which is a partial reconstruction of normal, locally and vastly injured vessel walls on the basis of fibrillar collagen grown cultures of endothelial cells. The kinetic curve of adhesion on collagen of gel-filtered human platelets reaches saturation within 40 min. During adhesion the shape of collagen bound platelets changes. Acetyl-salicylic acid and platelet free serum inhibit adhesion. Endothelial cells are nonadhesive to platelets; they do not affect the platelet adhesion to the intercellular areas covered with fibrillar collagen.

Animals↗
PubMed1976

[Characteristics of the upper cell surface in cultures of normal and SV-40 virus-transformed epithelium of mouse kidney. Study with the aid of scanning electron microscopy].

Central cells of the normal epithelial sheet are sparsely covered by microvilli. Numerous microvilli were seen in the regions of intercellular contacts. Marginal cells of sheets had a finely developed lamellar cytoplasm (lameloplasm) with smooth upper surface at their free margins. A transformed cell line (MPTR) resembled normal parent cells by its ability to form monolayered sheets in cultures. More microvilli of increased length appeared on the upper surface of central MPTR cells. The normal structure of lamelloplasm was changed at the free edge of the MPTR sheets. It is suggested that abnormal cell attachment to the substratum may be responsible for the altered cell surface morphology (increased length of microvilli, defective, structure of lamelloplasm) in the MPTR cultures.

Animals↗
PubMed1976

[Adhesive properties of the upper surface of fibroblasts and epithelium in cultures].

Adhesive properties of the upper surface of cultured normal and neoplastic epithelial cells and of fibroblasts were studied. It was shown that the attachment of prelabeled homo- and heterologous cells introduced into epithelial sheets were very low as compared with the free substratum and dense cultures of fibroblasts. The upper surface of cells of cultured anaplastic hepatoma 22a was adhesive for prelabeled cells. Morphological characteristics of the studied cultures were compared. It was found that low adhesiveness of the upper surface of epithelial sheets correlated with the formation of firm intercellular contacts which remained unbroken during migration into the wound. It is suggested that when epithelial cells make contacts with each other, their surface is subdivided into two types of regions: areas of low adhesiveness (the upper surface), and those of firm intercellular contacts. This may cause the formation of monolayer epithelial sheet.

Cell Adhesion↗
PubMedDate not supplied

[Selective isolation of actinomycetes of the genus Actinomadura from soil].

Some approaches to the selective isolation of actinomycetes of the genus Actinomadura from soil are described. The approach that involves thermal treatment of soil samples and their plating onto Gauze 1 medium with the antibiotics nystatin, nalidixic acid, and rubomycin provides for an increased amount of actinomaduras isolated from the soil actinomycete complex and for a decreased amount of streptomycetes.

Actinobacteria↗