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Biomedical subjects

P J Austin

Publications and source records attributed to P J Austin.

3 recordsLinked to original sources

Measurement of nitrous oxide concentrations in a simulated post anesthesia care unit environment.

The postanesthesia care unit (PACU) nurse works in close proximity to patients during early stages of recovery. It is during this time that the patient exhales the highest levels of anesthetic gases. The National Institute for Occupational Health and Safety has established standards for exposure to waste anesthetic gases which state that sampling should be done in the breathing zone of those most heavily exposed. Thus, it is important that a sampling methodology, data acquisition system, and statistical analysis technique be developed which accurately measures waste anesthetic gas levels at a point representing the breathing zone of nurses providing bedside care. A study was conducted to obtain an understanding of how the concentration of nitrous oxide varies with distance from a recovering patient. The study found that concentration of nitrous oxide decreases with distance from the patient; the patient's respiration increases the level of nitrous oxide at the location of the nurse; and the respiration of the nurse pulls the flow field toward them, increasing their exposure to the gas. The results show the inadequacy of attempting to measure levels of gas exposure for PACU nurses by a sampling protocol which calls for samples taken at random points in the room.

Air Pollutants, Occupational↗

Complex transcription of the Epstein-Barr virus BamHI fragment H rightward open reading frame 1 (BHRF1) in latently and lytically infected B lymphocytes.

Several cDNA clones containing the Epstein-Barr virus BamHI fragment H rightward open reading frame 1 (BHRF1) have been recovered from the tightly latent lymphoblastoid cell line IB4. These clones contain the 5' leader exons encoded in the major internal repeat 1 and the viral BamHI fragment Y, identified in the rightwardly transcribed viral mRNAs associated with the latent viral life cycle. In addition, a cDNA clone containing BHRF1 from the Burkitt lymphoma cell line Jijoye was also recovered and exhibits a distinctive splicing pattern. In vitro transcription and translation of BHRF1, followed by immunoprecipitation with Epstein-Barr virus-positive human sera, indicates that this viral antigen is expressed during infection. RNA blot analyses with a wide panel of lymphoblastoid and Burkitt lymphoma cell lines revealed a complex pattern of transcription. Hybridization data obtained with several probes is presented.

Cloning, Molecular↗

Structure, sequence and polymorphism in the HLA-D region.

Molecular analysis of the HLA-D region has uncovered a complex array of related genes encompassing a minimum of 6 alpha and 7 beta chain sequences. A high level of polymorphism is characteristic of the DQ alpha and beta genes, as well as DR beta. The DP genes, both alpha and beta, are also polymorphic, though to a lesser extent. The genes fit into the previously established loci: DP, DQ and DR, except for a newly-discovered sequence, DZ alpha, which is approximately equally related to all of the other alpha chain genes. Analysis of the polymorphism and evolution of the HLA-D region, by examination of the sequences, calls for several independent duplication events in the generation of this family of genes.

Base Sequence↗