Biomedical subjects
Publications and source records attributed to P K Shrivastava.
The distribution of AB0 and LH blood groups among five Punjabi populations from North India (Jat Sikh, Bania, Brahmin, Sikh Khatri and Hindu Khatri) is reported. Significant differences have been found in many cases regarding the distribution of AB0 and LH systems, especially between Sikh Khatris and Hindu Khatris, who are usually pooled for population genetic studies.
The study reports on the effect of 6 antimalarial drugs on red cell agglutination by Erythrina lithosperma. Phaseolus vulgaris and Ulex europaeus lectins. Some drugs inhibited agglutination while others potentiated it. Possible causes have been discussed.
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Serum haptoglobin, transferrin and albumin were studied electrophoretically in 50 patients with carcinoma of breast and in 50 normal healthy women. Results on haptoglobin show a preponderance of products of gene Hp1 (0.26) in patients as compared to controls (0.14). No variation was observed at the transferrin locus in either sample. The most notable finding was the detection of four cases of alloalbuminaemia (8%) in breast cancer Patients. The high incidence of alloalbuminaemia prompted us to extend our observations further. For this purpose sera from 41 patients was collected. One more case of alloalbuminaemia of the fast type was detected. However, in contrast to our first series of patients, quite high incidence (12.2%) of transferrin variants was recorded in this sample. Family studies were conducted on all the patients with protein anomalies.
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The frequency of antigen Dd-reactors has been recorded in Muslims and Buddhists from Ladakh, in Rana Tharus from Uttar Pradesh and in two samples of largely Jat Sikh origin from Punjab, all in India. The results show a wide range of variation, from 0% in the Rana Tharus to 25% in the Punjabi blood donors, of incidence of antigen Dd-reactivity in these populations.
The distribution of detectable antibodies against antigen Dd has been studied in rheumatoid arthritis, goitre, nephrotic syndrome, cirrhosis, gastrointestinal tract diseases, neurological diseases, liver and gall bladder diseases, breast cancer, respiratory diseases and cardiovascular diseases. Except in rheumatoid arthritis, breast cancer and nephrotic syndrome, where the incidence of antigen Dd-reactivity did not differ much from that in the control group, in all other disease it was significantly lower.
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Electrophoretic screening of sera from 550 individuals from Punjab, North India, revealed four cases of alloalbuminemia. Two albumin variants migrated slower and two migrated faster than the common albumin A. These variants were further analyzed by electrophoresis of their cyanogen bromide fragments to localize their molecular differences. One of the slow variants appears similar to, if not identical with, albumin B, with an altered cyanogen bromide fragment CNBr VII. The other slow variant appears to be a new variant (proposed name albumin Punjab) differing from albumin A in an altered fragment CNBr VI (which also occurs in albumins Kashmir and Adana) and in an altered fragment CNBr I. Among the fast variants, one has the same altered fragment CNBr V as albumin Naskapi, while the other appears to be a new variant (proposed name albumin Patiala) having an altered fragment CNBr VI. The presence of albumin Naskapi in Punjabis, North American Indians, and Eti Turks (previously reported) is consistent with the existence of a common ancestral population in which the mutation to Naskapi occurred before the migrations eastward and westward.
Results of a twin study on the distribution of LH (a new red blood cell membrane specificity) and ABO blood types have been reported among the Jat Sikh, Khatri, Ramdasia and a sample of mixed Punjabis from North India. The LH types are distributed bimodally, albeit with varying frequencies, in individuals with blood types other than O, in whom only the LH+ type occurred. The results suggest that the LH system could be usefully employed in population genetic studies, particularly in association with the ABO system.
187 Gampasati Kamma, 120 Pokanati Reddi and 98 Tangala Mala from Andhra Pradesh were examined for the LH red blood cell membrane specificity. Significant differences were observed in regard to the distribution of LH specificity in all the three populations. The results have been compared with those on some North Indian populations.
Serum albumin, ceruloplasmin, transferrin and haptoglobin were studied in 50 patients with both toxic and non-toxic forms of goitre. Albumin was monomorphic in all cases and its amount was substantially lower than normal in two patients. Ceruloplasmin was both qualitatively and quantitatively normal in all patients. No genetic variants of transferrin were encountered but 22% of the patients had this protein, at levels barely detectable by electrophoretic means. Such cases of hypotransferrinaemia occurred oftener in patients with the toxic form of goitre than in those with the non-toxic one. The distribution of haptoglobin types in patients was much the same as in the control blood donors. However, the patients had a greater incidence of ahaptoglobinaemia.
Three population groups, viz. Jat Sikh (192), Khatri (105) and Balmiki (108) were screened for haptoglobins, transferrins, Australia antigen, antigen Dd-reactivity and the ABO blood groups. The frequencies in Jat Sikhs, Khatris and Balmikis were 0.232, 0.186 and 0.140 respectively for Hp1 allele, 0.52%, 0.95% and 1.85% for Australia antigen, and 17.30%, 21.90% and 19.44% for antibodies against antigen Dd. The transferrins were all of type Tf C. The frequencies for the alleles A, B and O of the ABO system were 0.216, 0.239 and 0.545 in Jat Sikhs, 0.238, 0.320 and 0.442 in Khatris, and 0.196, 0.274 and 0.530 in Balmikis. Our tests showed these populations to be in Hardy-Weinberg equilibrium.
This new tympanoplasty technique with homo-ossicles (25 cases) has evolved as a reliable, effective, and durable technique to construct the middle ear conduction system when the total ossicular chain is missing. The isolated footplate is driven by two (primary and secondary) neotympanic membranes; hence this new technique is designated as "twin tympanoplasty." The initial hearing results (air-bone gap closure to 10 dB or less in 88% of the cases) have been good, and follow-up (six to 14 months) audiograms suggest that the hearing gain may survive the test of time. Toenail autograft could be one substitute for the ossicle as proved by satisfactory results in five cases.
This study was carried out among members of the Gaddi tribe of Himachal Pradesh in north India. The genetic traits reported here include ABO blood groups, haptoglobin, transferrin, albumin, Gc, Australia antigen and antigen Dd. The frequencies of genes p, q and r for the ABO system were 0.212, 0.290 and 0.428. The Hp1 allele frequency (0.331) was markedly higher and that of Gc1 (0.700) somewhat lower than in most north Indian populations. The incidence of Australia antigen was 1.25%. There were no antigen Dd reactors.
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A lectin obtained from seeds of Erythrina lithosperma detects a new specificity, called LH, on the human red blood cell membrane. Family investigations show conclusively that the specificity is under autosomal genetic control, with the gene determining the LH- type being dominant over its allele for the LH+ type.
Haptoglobin polymorphism has been studied in 148 Ladakhis -- a Mongoloid population inhabiting the northern-most region of India. With a frequency of 0.270 for the allele Hp1 the Ladakhis compare favourably with other Mongoloid populations.