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Biomedical subjects

Q Lin

Publications and source records attributed to Q Lin.

At least 217 records · Page 12Linked to original sources
PubMed1992

[Relation among syndrome of spleen deficiency and stagnation of liver-qi and electrogastrogram, gastric mucosal pathology and pulse-cardiovascular function in chronic gastric diseases].

In this research, Syndrome of Deficiency of Spleen (DS) and Stagnation of Liver-Qi (SLQ) as well as observations of electrogastrogram (EGG), gastric mucosal pathology and pulse cardiovascular examination were investigated in 56 cases of chronic gastric diseases (CGD). The results showed: (1) In SLQ with DS the amplitude of EGG was significantly higher than that of the control group (P < 0.01), it often indicated the active period of pathologic change, but in DS with SLQ, the amplitude of EGG was significantly lower than that of the control group (P < 0.01), it frequently denoted the static period. In case that DS equalled to SLQ, the amplitude of EGG was significantly higher than that of the control group (P < 0.01). It revealed that there were no significant difference between the patient's number of static and active periods (P > 0.05). It is valuable in Syndrome Differentiation of Deficiency and Excess and analysis of Superficiality and Origin in CGD. (2) Cardiovascular function was also affected in Syndrome differentiation of DS and SLQ. DS with SLQ manifested itself as reduction of left ventricle pump and declination for metabolism of microcirculation, they had significant difference as compared with the control group (P < 0.05), while SLQ with DS or equality between DS and SLQ had the normal function of left ventricle pump and metabolism of microcirculation, even though cardiac output were higher than that of control group (P < 0.05). It showed that the traditional Chinese medicine theory of interrelation among Heart and Spleen, Liver had their own hemodynamic bases.

Adult↗
PubMed1992

Volatile N-nitrosamines in salted fish samples from high- and low-risk areas for NPC in China.

Four carcinogenic volatile nitrosamines (N-dimethylnitrosamine, NDMA; N-diethylnitrosamine, NDEA; N-nitrosopyrroline, NPYR; and N-nirosopiperilidine, NPIP) were screened in twenty specimens of salted fish collected from areas in China with different nasopharyngeal carcinoma (NPC) mortality rates. The highest NDMA, NDEA and total N-nitrosamine contents (322.92, 50.27 and 373.19 micrograms/kg, respectively) were found in the samples from Sihui, one of the areas with highest NPC mortality. The lowest contents (12.64, 7.65 and 20.29 micrograms/kg, respectively) were seen in the samples from Shanghai, the area with the lowest mortality from NPC in the study. These results confirm that there are appreciable levels of nitrosamines in the salted fishes consumed by residents in high-risk areas of NPC in China.

Animals↗
PubMed1992

Members of the USF family of helix-loop-helix proteins bind DNA as homo- as well as heterodimers.

We have isolated human cDNA clones for USF2, a new member of the upstream stimulatory factor (USF) family of transcription factors. Analysis of these clones revealed the existence of highly conserved elements in the C terminal region of all USF proteins. These include the basic region, helix-loop-helix (HLH) motif, and, in the case of the human proteins, the C-terminal leucine repeat (LR). In addition, a highly conserved USF-specific domain is located immediately upstream of the basic region. Using in vitro translated proteins, we found that all members of the USF family bound DNA as dimers. The N-terminal portion of USF, including the USF-specific domain, was entirely dispensable for dimer formation and DNA-binding. However, deletion mutants of USF2 lacking the LR were deficient in DNA-binding activity. Interestingly, each of the USF proteins could form functional heterodimers with the other family members, including the sea urchin USF, which does not have a LR motif. This indicates that the conserved LR in human USF is not required for dimer formation, and influences only indirectly DNA-binding.

Amino Acid Sequence↗
PubMed1992

[Screening and distinguishing heavy chain variable region genes of McAb against encephalitis type B virus].

In order to prepare human-mouse chimeric antibody against encephalitis type B virus, hybridoma 51-8 cells secreting monoclonal antibody against the virus were used as material for isolating heavy-chain variable region gene of the McAb. High molecular weight DNA of the hybridoma cell was partially digested by BamHI, and then constructed a gene library containing 2 x 10(7) pfu with lambda EMBL-3 as vector. With cDNA of heavy-chain variable region of the monoclonal antibody as probe, nine positive plaques were screened from 360,000 plaques, which have been proven that they contained a fragment of heavy-chain variable region genes by dot hybridization and Southern hybridization. Four recombinants among the nine positive plaques were further distinguished with J11 probe containing J3, J4 and heavy-chain enhancer. After cutting by EcoR1, there was a 3.8 kb fragment in three recombinants as similar to that in liver cell and Sp2/0 cell, but not in the fourth recombinant (lambda 8a4) which contained a 4.5 kb fragment that did not present in liver cell and Sp2/0 cell. The results showed that the inserts in former 3 recombinants were non-rearranged fragment of heavy-chain variable region genes, but the insert in lambda 8a4 contained a rearranged functional variable region gene. The 4.5 kb fragment could not be hybridized with a probe containing J1 and J2, but contained VH, J3 or/and J4 and enhancer, that further proved it was a functional variable region gene. Therefore, the 4.5 kb fragment was isolated and subcloned in pUC 19, and its physical map was made.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1991

Isolation and characterization of a cDNA clone encoding the anti-viral protein from Phytolacca americana.

Phytolacca anti-viral protein (PAP) was purified from Phytolacca leaves and the N-terminal was sequenced. A cDNA library was made from mRNAs isolated from Phytolacca leaves and cDNA clones for PAP were identified using oligonucleotide probes derived from the N-terminal amino acid sequence. The PAP-cDNA clone was sequenced from both directions. The predicted amino acid sequence of PAP was compared with the amino acid sequences of other ribosome-inactivating proteins. The identities of these proteins to PAP ranged from 29 to 38%, and a region was found in each with a sequence similar to the PAP sequence (AIQMVSEAARFKYI). Southern blot analysis indicates that PAP is encoded by a multi-gene family.

Amino Acid Sequence↗
PubMed1991

[Propagation methods for nutmeg seeds].

The test was conducted on the interrelated factors of germination of Nutmeg seeds. The result indicated that germination occurred fast and exuberantly (up to 88.8%) when the fresh and plump seeds (collected in summer or early autumn) were shown and expedited in clean sand in the complete shade during May-August after being treated with wet sand for 96 hours.

Medicine, Chinese Traditional↗
PubMed1990

Spinal cord substance P mediates carbachol-induced cardiovascular responses from the rostral ventrolateral medulla.

Intrathecal (i.t.) infusion of substance P (SP) antagonist, (D-Pro2, D-Trp7,9)-substance P (20 micrograms), lowered blood pressure profoundly without any significant change of heart rate. The hypertensive and tachycardic responses elicited by microinjection of carbachol (25 ng/site) into the bilateral rostral ventrolateral medulla (rVLM) were blocked by i.t. infusion of SP antagonist. These data provided evidence that an excitatory cardiovascular effect induced by cholinergic system in rVLM may be mediated mainly by the SP receptors in spinal cord.

Animals↗
PubMed1990

Cytogenetic characterization of 20 lymphoblastoid lines derived from human individuals differing in bleomycin sensitivity.

Forty lymphoblastoid (lymphoid) lines were established from 42 volunteer blood donors, including healthy individuals and patients with head and neck carcinomas. Each peripheral blood sample was split into two portions, one for the establishment of a lymphoid line and the other for short-term culture, which was used to estimate bleomycin sensitivity by cytogenetic procedures. Twenty lymphoid lines were selected at random to compare bleomycin sensitivity with data obtained from short-term lymphocyte cultures. In each set, bleomycin sensitivity of lymphoid cells was similar to that of the lymphocytes. The lymphoid lines, which can be propagated for an unlimited supply of relatively homogeneous cellular material, will be useful for a variety of future investigations.

Bleomycin↗
PubMed1990

Rostral medullary cholinergic mechanisms and chronic stress-induced hypertension.

This work was designed to study the relationship between the cholinergic mechanisms in the rostral ventrolateral medulla (rVLM) and the incidence of hypertension induced by chronic stress. Under anaesthetized conditions, bilateral microinjection of scopolamine (1.18 nmol/site) into the rVLM produced a much greater depressor response in chronic stress-induced hypertensive rats than in normotensive rats. Similar bradycardic effects were observed in both the normotensive and the hypertensive rats when scopolamine was injected into the rVLM. Acetylcholine (Ach) content and choline acetyltransferase (ChAT) activity in rostral medulla were determined by radioimmunoassay both in the normotensive and the hypertensive rats. Ach content and ChAT activity increased significantly in the hypertensive rats, and such increase mainly occurred within the ventral part of the rostral medulla. These results suggest that the cholinergic mechanisms in the rVLM may be activated during chronic stress and such activation may be involved in the pathogenesis of the hypertension induced by chronic stress.

Acetylcholine↗
PubMed1990

[Rules for differentiating symptoms and signs of the heart-qi deficiency syndrome].

344 cases of Heart-Qi Deficiency Syndrome (HQDS) including 19 Kinds of disease were observed to explore the rule of differentiation of symptoms and signs for HQDS. The results showed that the common symptoms of HQDS were weakness, shortness of breath, and palpitation, etc. According to with or without complicated cardiovascular diseases, the patients were divided into two groups: group A with cardiovascular diseases, group B without that. In group A, the symptoms and signs of HQDS were the most frequent and early clinical manifestations, the associated symptoms and signs were blood stasis and attack of water-evil, etc. In group B, in whom often associated with nervous and emotional symptoms such as insomnia, dreamfulness, and amnesia, etc., which was due to the disturbance of emotional activities of heart. In order to avoid diagnostic confusion, the authors suggest that the HQDS patients with cardiovascular diseases would be diagnosed as HQDS; on the other hand, the HQDS patients without cardiovascular diseases would be diagnosed as disturbance of emotional activities of heart.

Coronary Disease↗
PubMed1990

[Preliminary study of aging and the senility index in traditional Chinese medicine].

According to the senile theory of TCM and the experience of the old generation, the authors chose 14 items which could represent the body's senility level. These items were graded. The total grade of these items were named "Senility Index of TCM" (SITCM). The age calculated from the regression equation of SITCM and age were named "Age of TCM", and the authors tried to estimate the body's senility level with it. According to the correlation analysis: with the rising of SITCM, lipid peroxide and viscosity of blood increase, function of heart, lung, brain decline and ATPase of RBC reduce, etc.

Adult↗
PubMed1989

Double-blind crossover controlled study on antiepilepsirine.

Antiepilepsirine (AES) is one of the derivatives of a Chinese folk prescription. It is a new antiepileptic drug (AED) synthesized in cooperation by Chinese medical and pharmaceutical workers. Pharmacological experiments on animal models prove that its antiepileptic action is marked, but there has been little evaluation of its clinical effects. We used the double-blind placebo-controlled crossover study which is generally acceptable as a proper method for new drug study. This study covered 58 epileptic children treated with classical AEDs and observed 6.5 months. Every patient took AES and placebo for 3 months each by random crossover on the basis of add-on therapy. The blood levels of AES and other antiepileptic drugs were determined regularly. The results show that there are no significant differences in clinical effects between AES and placebo in pediatric epilepsies as a whole, but AES is effective in tonic-clonic seizures (P less than 0.05), the most common type of seizure in the series. There are no significant differences in AED blood levels between the AES effective and ineffective groups. AES has no effect on the blood levels of other AEDs. AES is very safe, children given large doses (10 mg/kg/day) demonstrate no serious side-effects. It is suggested that there is potential improvement in patient psychological and cognitive status. This article also discusses the evaluation of new drugs for clinical effects, subject sampling, the criteria for efficacy evaluation and relationship between animal information and human outcome. The AES chemical structure is different from other well-known AEDs, this is a unique advantage.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗