PubMed Health⌕ Search

Biomedical subjects

Q Shang

Publications and source records attributed to Q Shang.

27 records · Page 2Linked to original sources
PubMed1998

[The inhibitory effect in vitro of salvia miltiorrhiza and tetramethyl pyrazine on the growth of fibroblasts].

In order to investigate the inhibitory effect of salvia miltiorrhiza (SM) and tetramethyl pyrazine (TP) on scartricial fibroblast, the hypertrophic scar tissue of chest was chosen for culture of fibroblasts, and the influence of SM and TP on fibroblasts was observed, The effect of the drugs on the growth of fibroblasts, on DNA synthesis of fibroblasts and on mitosis index of fibroblasts were all determined quantitatively. The results showed: 1. SM and TP could inhibit significantly the growth of the fibroblasts, the inhibitory effect was irreversible when the concentration of the drugs reached 5 mg/ml and 500 micrograms/ml respectively; 2. SM and TP could inhibit the absorption of 3H-TdR and this effect was correlated positively to the dosage of the drugs and; 3. SM and TP could reduce the mitosis index of fibroblasts. It was concluded that SM and TP had definite depressive effect on growth of fibroblasts which was correlated positively with the concentration of drugs and duration of application. The inhibitory effect of the drugs on fibroblasts was mainly through inhibition of synthesis of DNA.

Burns↗
PubMed1998

[The influence of salvia miltiorrhiza and tetramethyl pyrazine on DNA content of cicatricial fibroblasts and its cellular cycle].

In order to study the mechanism of the inhibitory effect of salvia miltiorrhiza (SM) and tetramethyl pyrazine (TP) on scar fibroblast, the DNA content of fibroblast and the all distribution in cellular cycle was measured by FCM. The hypertrophic scar tissue of chest was chosen for primary culture of fibroblast. Then this cultured cell was reacted with SM and TP. FCM was used to measure the DNA index and duration of cellular cycle. The results showed that: 1. SM and TP had little effect on DNA index, but when the concentration of drugs reached the threshold, they could increase the amount of fibroblasts in C2-M stage and the duration of G2-M stage was prolonged; 2. TP could also prolong the duration of S-stage; 3. SM and TP could prolong the multiplication time of fibroblasts and this effect was correlated postively with the dosage of drug. The conclusions were that the inhibitory effect of SM was the result of inhibiting the mitosis of cells and the cellular cycle be at a standstill in G2-M stage. The inhibitory effect of TP was due to the inhibition of synthesis and duplication of DNA and cellular mitosis, and the cellular cycle was also at a standstill in G2-M stage.

Burns↗
PubMed1998

[Advances in the research on repairing cartilaginous defects of synovial joint].

The repair of defects of articular cartilage has continued to be a difficult problem. This article provided a collective review from literature pertaining to the advances gained in the repair of cartilaginous defects. In the spontaneous repair, if the defect of the cartilage was less than 3 mm, might result in complete or partial repair, but in those the diameter was more than 3 mm, the defect could not be repaired by normal cartilage. Although the cartilaginous autograft could give good result, but it could not be widely applied because short of supply of the autogenous cartilage. Cartilagious allograft could not be taken to repair cartilaginous defect because of reaction from tissue rejection. The transplantation of periosteal or perichondral graft had been tried but was eventually abandoned because of poor long-term result. The transplantation of free chondrocytes might be a method of hope. In general, transplantation of free chondrocytes into the cartilaginous defect will be lost. The supply of autogenous chondrocytes was very limited, and the heterogenous chondrocytes would inflict immunoreaction after being transplanted. In late of 1980, a new concept of tissue engineering was proposed. The problem that a scaffold of appropriate material which could hold the free chondrocytes in place from being lost might undergo proliferation and differentiation into new cartilage was far from being solved. Although tissue engineering still had various problems needed further investigation, but it will probably be the main direction of development in this field.

Animals↗
PubMed1998

[Culture of chondrocytes seeded onto chitin scaffoldings coating with different materials: experimental study].

OBJECTIVE: To find the possibility of chitin used as scaffolds in tissue engineering. METHOD: Chondrocytes were cultured when they were seeded onto chitin meshcoated with polylactic acid (PLA) lecithin (LEC) and poly-l-lysine (PLYS). With light microscope and scanning electron microscope, the scaffolds in hydrophilia and absorptivity to chondrocytes and the function of the cells were observed. RESULT: LEC can change the hydrophilia of scaffolds. PLYS can strengthen the absorptivity of the scaffolds. and promote the matrices production of chondrocytes. CONCLUSION: The chitin coated with LEC and PLYS can be used as scaffolds in tissue engineering.

Animals↗
PubMed1995

Cadmium exposure among residents in an area contaminated by irrigation water in China.

River water used to irrigate arable land in Dayu County, Jiangxi Province, China, is polluted with cadmium from tailings and the wastewater of tungsten ore dressing plants. From information about the date on construction of ore dressing plants and an analysis of the annual growth rings of trees, we deduced that local residents have been exposed to cadmium for at least 25 years. Cadmium exposure was estimated based on a meal survey, which indicated that 99.5% of the oral cadmium intake came from rice and vegetables grown locally. The average oral intake of cadmium was calculated to be 367-382 micrograms/ day. Smokers had additional exposure from locally grown cadmium-containing tobacco. Cadmium concentrations in samples of urine (11 micrograms/g creatinine), blood (12 micrograms/l) and in the hair (0.11 microgram/g) of persons in the exposed area were high. The cadmium exposure lies in a range that can be considered to cause adverse renal effects in the long term.

Air Pollutants↗
PubMed1994

[Experimental study on adipocyte culture in vitro].

The adipocyte belongs to a kind of end cells. Most people take a skeptical attitude to its ability of proliferation in vitro. Based on foreign scientists' experience in this aspect. We isolated rats' adipocytes with percoll-density-gradient procedure. Using compound medium of DMEM and F12 with glucocorticoid, insulin and basic fibroblast growth factor (bFGF), we have succeeded in culturing fat cells in vitro.

Adipocytes↗
PubMed1989

[Epidemiological surveillance of filariasis after its being basically eradicated in Shandong Province].

This paper reports the results of epidemiological surveillance in areas where filariasis has been basically eradicated and control measures no longer adopted in Shandong Province. A 3-year longitudinal surveillance in 18 villages in 5 counties and a cross-sectional survey in 13 villages of 4 counties were carried out. The results showed that the average microfilaria rate was further reduced from 0.28% and 0.15% in 1980 to 0.19% and 0.05% in 1986, decreased by 32.1% and 66.7% respectively. 34.2% of the microfilaremic cases were found to be negative during the two-year follow-up. The positive rate of IFA and skin test was 34.2% and 38.6% respectively. In longitudinal surveillance. of the 240,992 Culex pipiens pallens dissected, 152(0.06%) were found to harbour different stages of larvae, among them, 5 were infective larvae from 4 vector mosquitoes. In cross-sectional survey, of the 3,875 Culex pipiens pallens dissected only 1 which had bitten immigrant from Zhejiang was found to contain one larvae at stage 1. No positive mosquito was found in the household of native villagers. No new infection was discovered at longitudinal and cross-sectional survey. The transmission of filariasis in these areas was interrupted. The results showed that in areas where filariasis has been basically eradicated, further control measures may not be instituted, but the management population movement and surveillance should be strengthened.

Animals↗
PubMedDate not supplied

Differential expression of CCAAT/enhancer-binding protein-delta (c/EBPdelta) in rat androgen-dependent tissues and human prostate cancer.

CCAAT/enhancer-binding protein delta (C/EBPdelta) is a nuclear transcription factor that regulates cellular growth and differentiation. In this study we demonstrate that C/EBPdelta gene expression is differentially regulated in rat androgen-dependent tissues and human prostate cancer. C/EBPdelta messenger RNA (mRNA) levels were very low in adult rat ventral prostate, epididymis, and testis. In ventral prostate and epididymis, expression of C/EBPdelta mRNA increased more than sixfold when testicular testosterone was eliminated by surgical castration or treatment with ethane-1,2-dimethanesulfonate (EDS). Testosterone replacement reduced C/EBPdelta mRNA levels to near control values in both tissues. CWR22 is a human prostate cancer xenograft that mimics biological characteristics of androgen-dependent and androgen-independent human prostate cancer. In androgen-dependent CWR22 tumors, expression of C/EBPdelta mRNA declined in response to castration. Both C/EBPdelta mRNA and protein levels increased following testosterone administration. However, C/EBPdelta mRNA and protein levels were variable in recurrent CWR22 tumors growing in the absence of testicular androgen for approximately 5 months. C/EBPdelta expression was also variable in androgen-independent human prostate carcinomas (n = 3), although mRNA levels were substantially lower than those in androgen-dependent tumors (n = 3). These studies demonstrate that androgen down-regulates C/EBPdelta levels in androgen-dependent rat tissues, but induces C/EBPdelta expression in androgen-dependent human prostate cancer. Deregulation of C/EBPdelta occurs when prostate cancer progresses to the androgen-independent state.

Androgens↗