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Biomedical subjects

R A Draughn

Publications and source records attributed to R A Draughn.

At least 19 recordsLinked to original sources
PubMed1996

Influence of biomaterial surface texture on bone ingrowth in the rabbit femur.

The purpose of this study was to examine both the histologic and the mechanical characteristics of bone apposition to an experimental surface, arc-deposited titanium, in a rabbit model and to compare them with those of four previously studied surfaces: one layer of cobalt-chromium beads, three layers of cobalt-chromium beads, plasma-sprayed cobalt-chromium, and uncoated titanium alloy. Bilateral cylindrical implants were press-fit into the lateral femoral condyles of 70 adult New Zealand White rabbits, which were allowed unrestricted activity and then killed at 6 or 12 weeks. The distal femora were harvested, radiographed, and prepared for either mechanical or histologic evaluation. All of the implants with coated surfaces had significantly greater shear strength than the implants of grit-blasted titanium alloy after both 6 and 12 weeks. After 6 weeks, maximum bone apposition occurred in the beaded surfaces. After 12 weeks, the shear strengths and bone apposition of implants of arc-deposited titanium and of one and three layers of cobalt-chromium beads were significantly greater than those of implants of plasma-sprayed cobalt-chromium and grit-blasted titanium alloy. The histologic studies correlated with the mechanical results. After 12 weeks, the bone apposition and mechanical stability of arc-deposited titanium were similar to those of a single layer of beads. There appeared to be no advantage to multiple layers of beads, and the plasma-sprayed cobalt-chromium and grit-blasted titanium surfaces showed lower shear strength and bone apposition than the other groups.

Analysis of Variance↗
PubMed1996

Effects of multiple sterilization on surface characteristics and in vitro biologic responses to titanium.

PURPOSE: This study evaluates the surface changes and effects on in vitro cell attachment and spreading brought about on prepared commercially pure titanium by multiple exposures to common sterilization methods. MATERIALS AND METHODS: Discs of commercially pure titanium were prepared to approximate the surface roughness of commercially available bone miniplates. Samples underwent sterilization by exposure to ultraviolet light; ethylene oxide sterilization (1, 5, or 10 cycles); or by steam autoclaving (1, 5, or 10 cycles). Representative surfaces from these sterilization groups were examined using a series of surface analytical techniques including scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), auger electron spectroscopy (AES), and contact angle measurements. Cell attachment assays using murine fibroblasts were then performed on titanium surfaces from each sterilization group and on tissue culture plastic controls. Sterilized surfaces contained O, C, and N contaminants, which affected surface energetics. Mean percent cell attachment values for each group were obtained for periods of up to 1 hour. Representative samples from each group were examined using SEM to ascertain cell spreading and morphology for each sterilization group. RESULTS: Ultraviolet (UV) sterilized surfaces showed no changes from the unsterilized state macroscopically or under SEM. UV surfaces showed cell attachment levels similar to control surfaces at all intervals, and a chronologic progression of cell spreading. Ethylene oxide-sterilized surfaces showed occasional bluish discoloration and a microscopic particulate contaminant, resulting in modest decreases in cell attachment levels without strong correlation to numbers of sterilization cycles. Autoclaved surfaces generally showed the greatest discoloration and heaviest particulate contamination. Cell attachment levels were lower, and cell spreading was diminished compared with the ethylene-oxide-treated group. CONCLUSIONS: Both ethylene oxide and steam autoclave sterilization contaminated and altered the titanium surface, resulting in decreased levels of cell attachment and spreading in vitro. Although corroborative in vivo experiments should be conducted, the results of this study indicate that some multiple sterilization regimens for metallic materials may pose serious biologic concerns.

3T3 Cells↗
PubMed1995

Histological and mechanical comparison of hydroxyapatite-coated cobalt-chrome and titanium implants in the rabbit femur.

The purpose of this study was to compare hydroxyapatite (HA)-coated titanium (Ti) and HA-coated cobalt-chrome (CoCr) implants in the distal femur of the rabbit by evaluating bone apposition and interfacial shear strength. Bilateral cylindrical implants with a plasma sprayed 50-microns thick HA coating were press-fit into the metaphyseal cancellous bone of the lateral femoral condyles in a transverse fashion, and the animals were sacrificed at 2, 4, and 8 weeks postimplantation. Mechanical strength of the interface between HA and bone was measured using the pushout method. For histologic analysis, the fractional linear extent of bone apposition was quantitated. No differences were found in the interfacial shear strength between the Ti and CoCr at any time period. The amount of bone apposition increased significantly at each time interval for both substrate metals, but there were no significant differences between the two substrates at any of the time periods studied. The HA-coated CoCr implants performed in a similar manner to the HA-coated Ti implants, both mechanically and histologically, suggesting that HA-coated CoCr implants deserve further study as a viable alternative to Ti for the biological fixation of total joint components in orthopaedic surgery.

Animals↗
PubMed1995

Application of a fluorescent redox dye for enumeration of metabolically active bacteria on albumin-coated titanium surfaces.

A bacterial staining method using fluorescent redox dye 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) is described for quantifying actively respiring bacteria that adhere to commercially pure titanium surfaces coated with cross-linked albumin. This has not been possible to date using ordinary DNA stains such as propidium iodide (PI) or Hoechst, both of which produce a very bright background. With this technique, it was demonstrated that the cross-linked albumin inhibited the adherence of Staphylococcus aureus and Staph. epidermidis to the titanium surface.

Bacterial Adhesion↗
PubMed1994

Characterizations of titanium implant surfaces. III.

There are several reports in the literature concerning the similarities and the differences between the oxide on cpTi and Ti-6A1-4V alloy; however, their biological sequelae are not entirely known. In this work, a series of surface characterization techniques were used in conjunction with short term in vitro biological assays to assess the effects of materials selection (cpTi and Ti alloy) on osteoblast-like cell responses. Surface analysis indicated that with the exception of oxide thickness, there were no significant differences in surface characteristics between the two implant materials. These results were reflected in the biological studies, where the levels of cell attachment and adaptation of the attached cells to the titanium surfaces were similar. These results are in general agreement with previous in vivo studies and continue to indicate that cpTi and Ti alloy are suitable, biologically compatible materials for fabrication of dental implants.

Animals↗
PubMed1994

Production of a standard closed fracture in the rat tibia.

In order to develop a technique for producing a standard closed transverse fracture in the rat tibia, the method of Bonnarens and Einhorn was modified and tested on 176 tibiae. A 0.9-mm Kirschner wire was inserted percutaneously near the tibial tuberosity into the intramedullary canal, and a tibial fracture was created at the junction of the middle and distal third of the tibia with a blunt guillotine driven by a dropped weight. Radiographs confirmed the production of a highly reproducible transverse mid-shaft tibia fracture in 90% of the fractures. Minimal comminution of the fracture or angulation of the Kirschner wires resulted. The Kirschner wires were removed without difficulty after the rats were put to death and did not disturb the fracture site. Mechanical testing and histological studies showed that a standard fracture healing process was obtained by using this method. The results indicate that this modified method creates a standard, reproducible transverse closed fracture of rat tibia.

Animals↗
PubMed1994

Evaluation of an adhesive system for amalgam repair: bond strength and porosity.

This study compared the shear bond strengths and the porosity at the bond sites of a spherical amalgam alloy to those of an admixed amalgam alloy, when they were bonded to like and unlike alloy specimens with or without a resin bonding material. The spherical alloy had higher bond strength and less porosity than did the admixed alloy, whether bonded to spherical or admixed alloy. The results suggest that use of a spherical alloy should be strongly considered when an amalgam restoration is to be repaired with an amalgam alloy, regardless of the type of alloy used in the original restoration. Use of a resin bonding agent did not increase bond strengths of amalgam to amalgam.

Analysis of Variance↗
PubMed1993

Evaluation of polycaprolaitone custom tray material.

Custom impression trays are necessary for accurate impressions in fixed prosthodontics when using polysulfide impression materials. These custom impression trays are needed to provide for a uniform thickness of impression material to minimize distortion. Custom impression trays have historically been made from acrylic resin. New materials have been developed as alternatives to acrylic resin, and this study evaluates one product currently available that is composed of polycaprolaitone. This study evaluates the modulus of elasticity of polycaprolaitone as compared with acrylic resin, and also evaluates the amount of permanent deformation of the new material when placed under a load. The elastic modulus of polycaprolaitone was tested using three-point bending of specimens placed in a universal mechanical test system. The elastic modulus of the new material was found to be significantly lower than that of acrylic resin. The next test evaluated the amount of permanent deformation of the polycaprolatione material when specimens approximating the shape and size of custom impression trays were placed under a load. These trays were placed in a universal mechanical test system and differing loads were placed on them. Results showed that although the trays deformed at relatively low loads, all deformation was fully recovered within a clinically insignificant period of time (less than 30 seconds). From these experiments it was concluded that although the polycaprolaitone material has a lower modulus of elasticity and will deform under relatively low loads, the material recovers completely, and thus the deformation would not interfere with the clinical performance of polycaprolaitone as a custom tray material.

Dental Impression Technique↗
PubMed1992

Short-term plasma-cleaning treatments enhance in vitro osteoblast attachment to titanium.

The purpose of this research was to characterize the in vitro cellular behavior of osteoblast-like cells on titanium surfaces prepared with argon plasma-cleaning (PC) treatments for various lengths of time. The highest levels of cell attachment were observed for surfaces which had been plasma-treated for one min. Surface analyses indicated that although PC treatments dramatically improved surface wettability, the presence of inorganic contaminants was observed with longer treatment times and may have interfered with cell attachment. Further work is suggested to investigate the longer-term phenotypic expression of osteoblasts when grown on implant surfaces.

Analysis of Variance↗
PubMed1990

Characterization of sterilized CP titanium implant surfaces.

Surface analysis techniques and in vitro biologic assays were used to characterize sterilized commercially pure titanium surfaces. Significant surface alterations were observed following sterilization treatments. These alterations led to decreased fibroblast cell attachment and altered cellular spreading phenomena compared to nonsterilized control surfaces.

Cell Adhesion↗
PubMed1987

Slow crack propagation in composite restorative materials.

The double-torsion test technique was used to study slow crack propagation in a set of dental composite resins including two glass-filled and two microfilled materials. The microstructure within each pair was the same but one of the resins was selfcured and the other photocured. The fracture behavior was dependent on the filler concentration and the presence of absorbed water. Wet materials fractured by slow crack growth in the range of crack velocity studied (10(-7) to 10(-3) m/s), and the microfilled composites, which contain a lower concentration of inorganic filler, had lower stress intensity factors (K1c) than the glass-filled composites tested. Dry specimens of the microfilled materials and the selfcured, glass-filled composite also showed unstable, stick-slip fracture behavior indicative of a crack blunting mechanism which leads to an elevation of the stress intensity factor for crack initiation over K1c for stable crack growth. The plasticizing effect of water increased the viscoelastic response of the materials measured by the slope of curves of slow crack growth. Analysis of fracture surfaces showed that cracks propagated at low velocities (10(-7) to 10(-5) m/s) by the apparent failure of the filler/matrix interfacial bond, and absorbed water affected the strength or fracture resistance of the interface. At high crack velocities the properties of the composite depend on the properties of the polymeric matrix, the filler, and the filler volume fraction, but at low velocities the interface is the controlling factor in the durability of these composites exposed to an aqueous environment.

Biomechanical Phenomena↗
PubMed1982

Linear dimensional changes in addition curing silicone impression materials.

Five vinyl polysiloxane addition curing impression materials were evaluated on the basis of linear dimensional change occurring as a function of time between making an impression and pouring the die. The dies were measured and compared to a master model to determine the linear change in the impression material. One material produced an overall larger die, but the greatest increase in size deviated from the master model by only 0.1%. Permagum putty-wash material consistently produced undersized dies, with the greatest change being 0.3%. Three materials randomly produced smaller or larger dies, differing from the master model by only 0.08% for the smallest die to 0.07% for the largest. No consistent pattern of increase or decrease in die size occurred with time. Dies produced at 168 hours were as accurate as those produced at 10 minutes.

Chemical Phenomena↗
PubMed1981

Effects of temperature on mechanical properties of composite dental restorative materials.

In the oral environment, dental restorative materials are exposed to temperatures ranging from 10 degrees to 50 degrees C. Since the properties of many polymeric materials are sensitive to temperature of this magnitude, it is important to define the effects of service temperature on the mechanical properties of polymer matrix dental composites. Six commercial composites were tested in compression at 11 temperatures, ranging from 2 degrees to 80 degrees C. The volume fraction of filler particles in the materials is either 0.45 or 0.55, and they contain a range of particle sizes and particle compositions. The tests show that ultimate strength decreases linearly with increasing temperature. Strength is higher for the lower volume fraction material and is decreased by the presence of a small percentage of very large particles. Elastic modulus and yield strength decrease sigmoidally with increasing temperature and depend only on particle volume fraction. In the clinically significant temperature range, ultimate strength decreases 14%, the decrease in elastic modulus is either 6 or 11%, and the yield strength decreases 45%. The data show that the temperature conditions of the oral environment can significantly affect the mechanical properties of composite dental restorative materials.

Biomechanical Phenomena↗
PubMed1981

Disruption of complement-mediated reactions by insoluble dentifrice ingredients.

It has been strongly suggested that the complement system plays a critical role in the pathological processes occurring in periodontal disease. Because individuals with this common disease are often instructed to brush their teeth more often and for longer periods of time, several commercial toothpastes were tested for their effects on complement-mediated reactions. In this study, six of the nine toothpastes tested activated the classical complement pathway, leading to C3 cleavage, as determined by crossed immunoelectrophoresis. The activating ingredients were contained in the insoluble fraction of the toothpastes. Of the toothpaste abrasives, Ca++ pyrophosphate was determined to have C3-converting activity. One of the toothpastes, which contains a particular type of amorphous silica abrasive, appeared to bind both native C3 and its conversion products. In general, disruption of complement-mediated functions in affected tissues could alter local immunological responses and interfere with healing. The in vitro studies described here suggest a need for clinical studies to ascertain the in vivo influences of dentifrice ingredients on complement-mediated reactions in periodontal disease.

Complement Activation↗
PubMed1980

Linear dimensional changes in elastic impression materials.

Four classes of elastomeric impression materials (polysulfide, polyether, silicone, and agar-agar) were evaluated on the basis of linear dimensional stability as a function of time between taking and pouring an impression of mounted teeth. Four polysulfide materials produced dies which were larger than the teeth and generally increased in size with impression storage time. One silicone material produced dies slightly smaller than the teeth, and the dies from another silicone were dramatically smaller with increasing storage times. A polyether material produced slightly smaller dies for up to four hours' storage time, then increasingly larger dies up to 24 h. The dies from a reversible hydrocolloid were larger than the teeth for storage times up to 30 min, and then decreased rapidly at longer times.

Agar↗