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R A Hardcastle

Publications and source records attributed to R A Hardcastle.

8 recordsLinked to original sources
PubMed1979

The staining pattern of collagen fibrils. Improved correlation with sequence data.

The periodic banding pattern of stained collagen fibrils observed in the electron microscopic can be correlated with the charge distribution deduced from the amino acid sequence. Earlier work used alpha 1 chain sequence data only. The present study incorporates alpha 2 as well as alpha 1 sequence data, so that the complete distribution of charged residues is used. Correlation is improved if it is supposed that the extrahelical terminal regions are contracted. The optimal value of the periodicity, D, (previously 232.3 +/- 0.5 residues using alpha 1 data only), is now 234.2 +/- 0.5 residues, assuming uniform spacing of residues in the helical body of the molecule. This value agrees better with values obtained by others from analyses of interactions between molecules, using sequence data alone. Using the improved value of D, the relative axial locations of the charged residues in the fibril are displayed. In this way, the charged residues contributing to each band in the fibril staining pattern can be identified.

Amino Acid Sequence↗
PubMedDate not supplied

A computer method for the comparison or the quantitative similarity between normal and abnormal collagen or between sequence data and experimental data.

In this paper, a method is described to correlate collagen structural data obtained by electron microscopy with chemical sequence data, or to compare two experimental sets of data. In this respect, collagen provides a valuable model system, firstly for studying the chemical basis of ultrastructure and the mechanisms of various treatments on a protein, and secondly for detecting and locating the alterations in collagen fibril structure produced by a collagen disorder.

Amino Acid Sequence↗
PubMedDate not supplied

CUSUM: a credible method for the determination of authorship?

Authorship attributions based upon the CUSUM (Cumulative Sum Analysis) method are still being presented in court proceedings as evidence despite the findings of several independent researchers that the method is unreliable. This paper describes the shortcomings of the method, presents further examples of its failures and summarises the findings of other researchers. The CUSUM method is seen to be discredited and should not be accepted as providing reliable evidence of authorship of either the written or the spoken word.

Authorship↗