Biomedical subjects

R Cai

Publications and source records attributed to R Cai.

At least 37 records · Page 2Linked to original sources

PubMed1998

Effects of fibroblast-mediated interleukin 3 and interleukin 6 gene therapy on hematopoiesis in mice treated with 5-fluorouracil.

Fibroblast-mediated cytokine gene therapy has proven to be a promising strategy for restoring hematopoiesis following repeated chemotherapy. Interleukin 3 (IL-3) and interleukin 6 (IL-6) can synergistically promote the recovery of hematopoiesis following chemotherapy. In this investigation, combined use of fibroblast-mediated IL-3 and IL-6 gene therapy was tested for hematopoietic effects on mice with or without 5-fluorouracil administration. The results demonstrated that combined therapy with IL-3 gene-modified NIH3T3 cell (NIH3T3-IL-3) and IL-6 gene-modified fibroblast NIH3T3 cell (NIH3T3-IL-6) implantation achieves obvious stimulation of hematopoiesis in normal mice and accelerates recovery of hematopoiesis. In normal mice the quantities of platelets, neutrophils, and total white blood cells in peripheral blood increased significantly after the combined implantation of NIH3T3-IL-3 and NIH3T3-IL-6 cells. The numbers of colony-forming unit (CFU) granulocyte/macrophage (CFU-GM) and CFU megakaryocyte (CFU-MK) formed by stem cells in bone marrow was significantly higher after the combined implantation of NIH3T3-IL-3 and NIH3T3-IL-6 cells than after the implantation of NIH3T3-IL-3 alone, NIH3T3-IL-6 alone, or neomycin gene-modified NIH3T3 cells. In hematopoiesis-depressed mice induced by preinjection with 5-fluorouracil at the dose of 150 mg/kg before cell implantation, the platelets, neutrophils, and white blood cells showed accelerated recovery, and the numbers of CFU-GM and CFU-MK formed by bone marrow cells were also markedly higher after the combined implantation of NIH3T3-IL-3 and NIH3T3-IL-6 cells than in control groups. Our data show that combined use of fibroblast-mediated IL-3 and IL-6 gene therapy may be of clinical relevance for the recovery of hematopoietic depression for patients after chemotherapy.

3T3 Cells↗

PubMed1998

First trimester pregnancy loss associated with varicella zoster virus infection: histological definition of a case.

Pathological demonstration of varicella infection in first trimester aborted tissue is reported. A 24-year-old primigravida manifested chickenpox infection about 38 days after her last menstrual period or at 24 days age of the embryo. The conceptus survived another 4 to 5 weeks. The macerated embryo and placental tissue revealed nuclear changes consistent with varicella infection. Immunohistochemical stains and electron microscopy were confirmatory.

Adult↗

PubMed1998

Augmentation of hematopoiesis by fibroblast-mediated interleukin-6 gene therapy in mice with chemotherapy.

Murine fibroblast NIH3T3 cells engineered to secrete interleukin-6 (NIH3T3-IL-6) were implanted intraperitoneally into mice and observed for their hematopoietic effects with or without 5-fluorouracil (5-FU) administration. In normal mice, the platelet and neutrophil counts in peripheral blood increased significantly after implantation of NIH3T3-IL-6 cells, but the total white blood cell numbers showed no obvious elevation. The granulocyte-macrophage colony-forming unit (CFU-GM) and megakaryocyte colony-forming unit (CFU-MK) numbers formed by stem cells in bone marrow and spleen were also found to be significantly increased after implantation of NIH3T3-IL-6 cells when compared with those in mice after implantation of NIH3T3 cells transduced with neomycin gene (NIH3T3-Neo). To observe the protective effects of NIH3T3-IL-6 cells on hematopoietic depression in chemotherapy-treated mice, the mice were preinjected with 5-FU at a dosage of 150 mg/kg before implantation of NIH3T3-IL-6 cells. The platelet and neutrophil counts showed accelerated recovery after implantation of NIH3T3-IL-6 cells. The numbers of CFU-GM and CFU-MK in bone marrow and spleen were also found to be markedly increased in hematopoietic-depressed mice when compared with those in mice implanted with NIH3T3-Neo cells. These data suggest that fibroblast-mediated IL-6 gene therapy, which can augment hematopoiesis in mice with or without chemotherapy, will be of great help in the recovery from hematopoietic depression after chemotherapy.

3T3 Cells↗

PubMed1998

Experimental study of lymph node auto-transplantation in rats.

OBJECTIVE: To observe the restoration of structure and function of auto-transplanted lymph nodes. METHODS: Inguinal lymph nodes in Spregue-Dawley (SD) rats were auto-transplanted by free implantation, or with an intact vascular pedicle, or by free transplantation with microvascular anastomosis, to the popliteal fossa where lymph nodes were removed. The observation methods included emission computerized tomographic (ECT) scanning, staining of China ink and methylthionine chloride to observe the histological changes. RESULTS: After four weeks, these vascularized nodes showed normal histological appearances and spontaneously reestablished afferent and efferent lymphatic reconnection with the surrounding lymphatic vessels. ECT lymphoscintigraphy with 99mTc-Dx showed that vascularized lymph nodes had restored their normal function. CONCLUSION: Vascularized lymph node transplantation is a useful method for draining extremity lymph edema.

Animals↗

PubMed1998

[Effects of an extract of salviae miltiorrhizae (764-3) on structural remodeling of intra-acinar pulmonary artery in pulmonary hypertension due to chronic hypoxia and monocrotaline in rats].

OBJECTIVE: To investigate the effects of an extract of salviae miltiorrhizae (764-3) on the percentage of intra-acinar muscular artery (MA), partal MA(PMA) and nonmuscular MA(NMA) and collagen deposition in the media of MA in rats with hypoxia and MCT-induced pulmonary hypertension. METHOD: Wistar rats were divided into 12 groups. The hypobaric hypoxic 51 kPa (1 kPa = 7.5 mmHg) and MCT (60 mg/kg intraperitoneal injection) rats were treated with 764-3 (20 mg/kg), beta-aminopropionitrile (BAPN, 150 mg/kg) and 764-3 (40 mg/kg), respectively. At each time point, the hemodynamic and morphological parameters were measured. RESULT: After hypoxia for 2 weeks, the percentage of MA increased (P < 0.001), of NMA decreased (P < 0.001) and of PMA no obvious change; and after 4 weeks, the above changes became to be more striking and the collagen content in the media of MA increased (P < 0.001). The MCT-treated rats had more severe changes than the hypoxic rats. Both 764-3 and BAPN could inhibit the changes, decreased the collagen content and BAPN was more effective than 764-3(P < 0.05). CONCLUSION: 764-3 can partially inhibit collagen deposition in pulmonary arterioles attenuate hypoxia and MCT-induced pulmonary hypertension, reduce the percentage of MA, and has a moderate influence on collagen deposition as compared with BAPN.

Animals↗

PubMed1998

[Effect of 764-3 on ventricular collagen deposition in pulmonary hypertension induced by chronic hypoxia and monocrotaline in rats].

OBJECTIVE: To observe the effect of 764-3 on ventricular collagen deposition in pulmonary hypertension induced by chronic hypoxia or monocrotaline (MCT) in rats. METHODS: Wistar rats were divided into 10 groups. The hypobaric hypoxia group received 764-3 20 mg/kg once daily subcutaneously and the MCT group treated with 764-3 40 mg/kg once daily. The hemodynamic and biochemical parameters were measured. RESULTS: At the time of 2 weeks and 4 weeks after treated with hypoxia or MCT, the pulmonary arterial pressure (PAP) of rats raised significantly and the right ventricular collagen content (RVCC) increased markedly. 764-3 could reduce the raised PAP induced either by hypoxia or MCT. It could lower the elevated RVCC of hypobaric hypoxia group at 2 weeks from 76.3 +/- 13.3 to 59.4 +/- 11.4 (P < 0.01), and the RVCC of MCT group lowered from 293.4 +/- 73.9 to 210.1 +/- 37.6(P < 0.01). CONCLUSION: 764-3 could partially prevent right ventricular collagen deposition induced by hypoxia and has therapeutic effect on that induced by MCT.

Animals↗

PubMed1998

[Clinical study of pulmonary encephalopathy with integrated traditional Chinese and Western medicine].

OBJECTIVE: To explore the clinical effectiveness of integrated traditional Chinese and Western medicine (TCM-WM) on pulmonary encephalopathy. METHODS: Eight three patients of pulmonary encephalopathy were allocated randomly into the TCM-WM group and the control group. The therapeutic method for the control group including continuous low flow oxygen intake with mechanical ventilation available when necessary, antiasthmatics and expectorants improve ventilation, sufficient and combined intravenous infusion of antibiotics, and prescription of diuretics for dehydration under the therapeutic principle of mild, small dosage, combined and intermittent in use. For the TCM-WM group, besides above-mentioned medication, Chinese medicine of clear up the Heat and toxin, tranquilizing, restore consciousness and induce resuscitation, bactericiding and expelling their toxin. The anti-pulmonary-encephalopathy injection was given as soon as possible. RESULTS: The total effective rate of the TCM-WM group reached 83.0% and was higher than that of the control group (63.9%) significantly (P < 0.05). Time for restoring consciousness in the former was also shorter than that of the latter significantly (P < 0.01). CONCLUSION: This study provided both method and experience for the treatment of pulmonary encephalopathy with TCM-WM.

Aged↗

PubMed1998

[Effects of 764-3 on collagen deposition in hypertensive pulmonary arteries in hypoxic rats].

OBJECTIVE: To investigate the changes of collagen deposition in pulmonary arteries in chronic hypobaric hypoxic rats and the roles of 764-3. METHODS: Wistar rats were divided into 10 groups. The hypoxic (50.7 kPa) rats were treated with 764-3 (20 mg/kg, once daily) and beta-aminopropionitrile (BAPN, 150 mg/kg, twice daily), respectively. At the end of treatment, the hemodynamic morphological and chemical parameters were measured. RESULTS: Comparing with the normals, hydroxyproline (HYP) content of extrapulmonary artery (EPA) increased obviously in hypoxic rats, the smooth muscle cells (SMC) in the media of muscular intra-acinar pulmonary artery showed hypertrophy with change in phenotype, and a striking increment of collagen accumulation (P < 0.001); 764-3 could reduce HYP content of EPA (P < 0.05-0.001), and most of SMC in the media became thin and elongated with reversion of change of SMC phenotype, and collagen accumulation was reduced (P < 0.001). In addition, BAPN was more effective than 764-3 (P < 0.05). CONCLUSIONS: Excessive collagen accumulation in pulmonary arteries plays an important role in the maintenance of hypoxic pulmonary hypertension. 764-3 can partially inhibit collagen deposition in pulmonary arteries, attenuate hypoxic pulmonary hypertension, and has a moderate influence on collagen deposition in compared with BAPN.

Aminopropionitrile↗

PubMed1998

[Math-model of efficient cultivation of Angelica sinensis].

Using two-time general rotation-constitution design, the math-mdele has been founded about Angelica sinensis prodution efficiency and quantity of nitrogen, phosphorus and potassium to use by doing experiments in many different spots and production demonstration for testing and verifying. Accordin to the modle, computer is used to simulate the better, through which people can get fresh Chinese angelica over 500 Kg per mu and also net income over 500 yuan per mu. It shows that this is the best combination. 95% density riliability for this plan is 6860-7600 plants, pure nitrogen 18.75-22.2 Kg, P2O5 9.1-11.1 Kg and K2O 4.46-5.37 Kg One mu. Proportion for nitrogen, phosphorus, potassium is 1:0.49:0.24.

Angelica↗

PubMed1997

Early occurrence and prognostic significance of p53 alteration in primary carcinoma of the fallopian tube.

The pathogenesis of primary carcinoma of the fallopian tube (PCFT) is poorly understood. Tumor suppressor p53 gene alterations are common in human malignancies, but their role in the tumorigenesis and survival of PCFT is undefined. The objectives of this study were to define the occurrence and prognostic role of p53 alteration in PCFT and to examine the efficiency of immunohistochemistry (IHC) in detecting p53 alteration in PCFT. Fifty-two PCFT and 10 normal fallopian tubes were examined for p53 alteration by IHC and polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP). The Kaplan-Meier method was used to derive the survival function, while the log-rank test was used to compare curves for two or more groups. Other patients' characteristics were analyzed by two-tailed Fisher's exact tests. IHC correlated well with PCR-SSCP with 100% sensitivity and 83.3% specificity for detecting p53 alteration in this study. Thirty-one of 52 (57%) PCFT showed p53 alteration. Alteration of p53 occurred in all stages of PCFT with a similar incidence in carcinoma in situ and late-stage disease. Alteration of p53 was related to tumor histologic type. Significant survival difference was noted between advanced and early clinical stages but no such difference was identified among different tumor grades. Compared to the p53-nonaltered group, the presence of p53 alteration in PCFT was related to significantly decreased patient survival (RR = 6.8, 95% CI = 2.9-16.2) in multivariate analysis. In the subgroup of patients with residual tumor after surgery, those with p53-altered tumors had a significantly decreased survival compared to those with p53-nonaltered group (RR = 7.4, 95% CI = 1.9-28.2). Alteration of p53 is common and IHC is an efficient method to detect p53 alteration in PCFT. Shorter survival is associated with p53 alteration which is an independent marker for the disease in this study. Alteration of p53 may be an early event in tubal tumorigenesis and may play an important role in the development of PCFT. Whether detection of p53 alteration may serve as an indicator of high-risk patients for whom more aggressive adjuvant chemotherapy may be considered needs to be explored in the future.

Aged↗

PubMed1997

Cutaneous malignant melanoma in the young.

Between the years 1967 and 1993, 3246 patients were diagnosed with malignant melanoma at Frenchay Hospital, Bristol. This paper reports 47 patients, 21 years of age or under, including 10 preadolescent cases under 14 years of age. It represents a further follow-up of a cohort originally published from this centre in 1986 in addition to 18 new cases. Most (89%) of the lesions occurred on the trunk and extremities, with females showing a predominance of lesions on the lower limbs. 83% of the melanomas were of the superficial spreading type: 72% invaded to Clark level III and IV. Thickness ranged from 0.29 mm to 50.00 mm (median 1.20 mm). Ulceration was present in 17% of cases and 32% of melanomas arose within a pre-existing small congenital melanocytic naevus. Overall 5-year survival was 81%, with a mean follow-up of 8.5 years. Ulceration and tumour thickness of greater than 1.5 mm were associated with a poor prognosis.

Adolescent↗

PubMed1997

[Study on standard of patch test for cosmetic allergens].

39 allergens, chemical ingredients commonly used in cosmetics in China, were tested. The safe concentration of allergens was determined in experiments on animals. On the basis of the above results and referring to the literature, the concentration of allergens used in patch test for human was designed and the patch test was carried out on 10 volunteers by routine method. Finally the concentration which gave no reaction in all 10 volunteers was verified in patients with eczema or contact dermatitis. The rate of positive reactions for each allergen was raged from 0 to 12% with an average 3.4%, According to the results of three above mentioned experiments the series of allergen standards for patch test was established. It is used as an appendix to the document for diagnostic criteria and principles of management of consmetic diseases.

Allergens↗

PubMed1997

[A three-month follow-up study of excimer laser photorefractive keratectomy for myopia].

In order to evaluate the efficacy of photorefractive keratectomy (PRK) in the treatment of myopia, we summed up and analysed the 3-month follow-up data on 145 cases treated in our hospital. Prior to PRK, refractive dioptal was -1.5 to -11.5; visual acuity was less than 0.1 in 137 cases (94%) and less than 0.5 in 8 cases(6%). One month after operation, the mean refractive dioptal was +0.98 +/- 0.88; visual acuity increased to > or = 1.0 in 95 cases (66%) and to > or = 0.5 in 142 cases(98%). Three months after operation, the mean refractive dioptal was -0.13 +/- 1.09; visual acuity increased to > or = 1.0 in 104 cases(72%) and to > or = 0.5 in 135 cases(93%). There was no severe complication noted in all cases. The results demonstrate that PRK is and effective, safe and predictable treatment for myopia.

Adolescent↗

PubMed1997

The determinative role of intraoperative echo in tricuspid valvuloplasty.

A transesophageal echocardiography (TEE) was used in monitoring the operation of tricuspid valvuloplasty. Nine patients with rheumatic heart disease were studied. The results showed that intraoperative TEE was important on the improving method of tricuspid annuloplasty, it help to determine surgical procedure, control annulus-reducing procedure and assess the result immediately after operation. The results suggested that intraoperative TEE can be widely used in valvuloplasty in future.

Adult↗

PubMed1996

Secretory expression of a single-chain insulin precursor in yeast and its conversion into human insulin.

A synthetic single-chain porcine insulin precursor (PIP) gene and an alpha-mating factor leader sequence (alpha MFL) gene obtained by the PCR method are inserted between the promoter and 3'-terminating sequence of the alcohol dehydrogenase gene ADH1 in plasmid pVT102-U to form plasmid pVT102-U/alpha MFL-PIP. The single-chain insulin precursor is expressed and secreted to the culture medium by Saccharomyces cerevisiae transformed by pVT102-U/alpha MFL-PIP. The precursor is purified and converted into human insulin by tryptic transpeptidation. The purified human insulin is fully active and can be crystallized. The overall yield of human insulin is 25 mg per liter of culture medium.

Base Sequence↗

PubMed1996

[Experimental study on in vivo hematopoietic regulation of IL-6 gene-transfected tumor vaccine].

In the present study, we investigated the effects on hematopoiesis of inactivated vaccines prepared from mouse erythroid leukemia cells (FBL-3) transfected with IL-6 gene. After treatment with IL-6 gene-transfected FBL-3 cells, the platelet count in mice started to increase at day 4, reached its maximum at day 10 and lasted for 25 days. The neutrophil count also increased significantly, but WBC count remained unchanged. The CFU-GM and CFU-MK were elevated to some extent in bone marrow and spleen. These results indicated that in addition to augmentation of antitumor immunity, IL-6 gene-transfected tumor vaccine could promote hematopoietic functions in bone marrow and spleen, and elevate platelet and neutrophil counts in peripheral blood. This approach to gene therapy may be applicable in leukemia treatment, especially for thrombocytopenia related to leukemia or chemotherapy.

Animals↗

PubMed1996

[Acceleration of hematopoietic reconstitution in mice transplanted with syngeneic bone marrow cells by IL-6 gene therapy].

In the present study, the potential of fibroblast-mediated IL-6 gene therapy for accelerating hematopoietic reconstitution after syngeneic bone marrow transplantation (BMT) in BALB/c mice subjected to total body irradiation at a dose of 7 Gy was investigated. We observed that not only recovery of platelets, WBCs, CFU-GM or CFU-MK in bone marrow in mice treated with IL-6 gene therapy in combination with transplantation of 10(7) syngeneic bone marrow cells was faster than that in mice treated with IL-6 gene therapy in combination with transplantation of 10(6) or 10(5) syngeneic bone marrow cells, but also the number of CFU-S or survival rate in mice treated with IL-6 gene therapy in combination with transplantation of 10(7) syngeneic bone marrow cells was significantly higher. Nevertheless, the number of platelets, WBCs, CFU-GM or CFU-MK in bone marrow, CFU-S and survival rate in mice treated with IL-6 gene therapy in combination with transplantation of syngeneic bone marrow cells were elevated more significantly than that in mice transplanted with syngeneic bone marrow cells alone. These data demonstrated that IL-6 gene therapy could markedly augment hematopoietic reconstitution after syngeneic bone marrow transplantation and the more bone marrow cells transplanted, the better was the effect.

3T3 Cells↗

PubMed1996

[Effects of smoking on lung function in populations of Beijing and Guangzhou].

OBJECTIVE: To analyse the relationship between cigarette smoking and lung function in populations of Beijing and Guangzhou. METHODS: Complete lung function and smoking habit data were recruited from 7983 men and women aged 35-59 in or around Beijing and Guangzhou. Comparisons were made between smokers and never smokers. RESULTS: The mean values of FEV1/H2, FEF/H2 and FEV1/FVC are all lower in smokers than in never smokers for male smokers, the observed mean value of FEV1/H2 for each age group in smaller than the respective predicted value, and its descending trend with aging is more obvious than the predicted value when re-examination after 2-4 years; furthermore, smokers had higher prevalences of respiratory symptom and COPD. CONCLUSION: Cigarette smoking is harmful to lung function.

Adult↗