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R Colimon

Publications and source records attributed to R Colimon.

At least 37 records · Page 2Linked to original sources
PubMed1987

[Diagnostic value of the study of anti-Chlamidia antibodies in salpingitis. 379 cases].

Antibodies to Chlamydia were assayed by complement fixation (CF) and inclusion indirect immunofluorescence (IFI) in sera collected from 379 patients with salpingitis: 30.3% of the patients had total and IgM antibodies at IFI and CF antibodies (profile I); 26.6% of the patients had total and IgM antibodies at IFI without CF antibodies (profile II); 31.6% of the patients had only total antibodies at IFI without specific IgM and without CF antibodies (profile III); 11.3% of the patients were Chlamydia antibody negative (profile IV). In the control group of 50 pregnant women apparently non infected, the profile distribution was 2% profile I, 8% profile II, 38% profile III, and 54% profile IV. Detection of IgM antibodies to Chlamydia trachomatis in 57% of patients with salpingitis, taking only one specimen, suggested recent or active chlamydial infection. CF antibodies indicated diffuse infection. Total antibodies correlated well with IgG antibodies detected by ELISA. Their finding was by no means diagnosis for Chlamydia being the cause of tubal infection, although titers observed in salpingitis patients were higher than in controls.

Adolescent↗
PubMed1987

[Early detection of cytomegaloviruses from pathological matter and feces].

CMV can be isolated within 24 or 48 h post-infection of human diploid fibroblasts cells cultures by detection of immediate early antigen with immunological techniques using E13 monoclonal antibody. Optimal enhancement of CMV detection is obtained by centrifugation at 4,000 g for 45 minutes of specimen inoculum onto cell culture monolayers. Rapid immunodiagnosis can be applied to cells from clinical specimens faster than with culture but it is less sensitive. This rapid diagnosis of CMV infection enables early prescription of appropriate antiviral chemotherapy.

Antigens, Viral↗
PubMed1987

Conservation and map location of human cytomegalovirus strain Ad-169 transforming sequences in the DNA of clinical isolates.

All DNAs from human cytomegalovirus studied (57 fresh isolates, 3 reference strains) showed hybridization under stringent conditions to a plasmid containing strain Ad-169 transforming sequences. Analysis of hybridization patterns obtained with DNAs digested by three different enzymes allowed construction of a global map for this highly conserved area of the genome.

Cell Transformation, Neoplastic↗
PubMed1985

Demonstration of the colinearity of human cytomegalovirus genomes and construction of restriction maps of unknown isolates using cloned subgenomic fragments.

In this study, we have established the colinearity of human cytomegalovirus (HCMV) genomes using stringent conditions of DNA-DNA filter hybridization of HCMV HindIII fragments and cosmid-cloned AD169 strain HCMV DNA fragments. Large cosmid-cloned fragments of AD169 DNA were used for the preparation of radioactive probes by nick translation. These probes were hybridized to HindIII digests of DNA from three fresh isolates of HCMV and to that of the Davis strain. Using published HindIII restriction maps for the AD169 strain as a reference, the results obtained by hybridization allowed us to construct HindIII restriction maps for the genomes of the three fresh isolates. Confirmation of our methodology was found in the correspondence between the HindIII map we constructed for the Davis strain and that published previously. Furthermore, it is shown that variation in the restriction profiles of the unique regions of the genome are due to the absence or gain of restriction sites, and not to a rearrangement of fragments. This technique allows rapid construction of physical maps of the DNA of any fresh isolate for a given restriction enzyme provided the corresponding restriction map of a strain to be used as reference is available.

Base Sequence↗
PubMed1984

Translocation of antibiotic resistance markers of a plasmid-free Streptococcus pyogenes (group A) strain into different streptococcal hemolysin plasmids.

Wild-type strain A454 (Streptococcus pyogenes) transferred en bloc its erythromycin (Em) and tetracycline (Tc) resistance markers into several plasmid-free streptococcal recipients. No plasmid DNA was detected in either the wild-type or the transconjugant strains. Crosses were performed between A454 and S. faecalis Rec+ or Rec- recipients carrying hemolysin-bacteriocin plasmids, pIP964 or pAD1 . The Em Tc-resistant transconjugants obtained harbored either the parental plasmid or an Em Tc resistance plasmid derived from pIP964 or pAD1 . The restriction endonuclease analysis of 12 derivative plasmids showed insertions of various sizes into different fragments of pIP964 or pAD1 . A454 and the Em Tc-resistant plasmid-free transconjugants were found to contain two EcoRI DNA fragments, that shared homology with 32P-labeled pIP1077 , one of the Em Tc resistance derivative plasmids, but not with 32P-labeled pIP964 . No homology was detected between pIP1077 and the cellular DNA of the antibiotic-susceptible recipients.

Conjugation, Genetic↗
PubMed1984

Comparison of six methods for detecting human rotavirus in stools.

The following six methods for detecting rotavirus in human faecal samples were compared: electron microscopy, immune electron microscopy, immunofluorescence in cell culture, two enzyme immunoassays (Rotazyme, Enzygnost ) and a latex agglutination test ( Rotalex ). Specimens were collected from 112 children with diarrhoea. The relative sensitivities of the different assays for human rotavirus were as follows: electron microscopy, 84%; immunofluorescence, 86%; Rotalex , 88%; Rotazyme, 89%; immune electron microscopy, 93%; Enzygnost , 98%. According to our findings Enzygnost is the most sensitive method, but Rotalex is more valuable for screening a small number of faecal samples. No false-positive results were observed in the two enzyme immunoassays or in Rotalex .

Child, Preschool↗
PubMed1984

[Direct diagnostic methods for the demonstration of cytomegaloviruses in the blood].

CMV can be detected in blood with two techniques: isolation of CMV from the buffy-coat on human embryonic fibroblasts; CMV genome detection by DNA hybridization technique. Isolation of CMV from blood plated on embryonic fibroblasts necessitates a delay of 10-30 days. Using monoclonal antibodies we have developed a 96 hours test to diagnose viremia. Fibroblasts grown on coverslip in tubes are inoculated with buffy-coat. Indirect immunofluorescence test is performed using the monoclonal antibodies E 13, 48 and 96 hours post-infection. DNA-DNA hybridization technique necessitates purification of CMV DNA. CMV DNA is then labelled with 32 P by nick-translation. This labelled DNA is used as probe for detection of CMV genome in DNA extracted from leukocytes. The hybridization is virus-specific. Cytomegaloviremia correlates with active infection and is not indicate of the carrier state of blood donors. CMV genome research in blood can potentially identify infective donors. Further studies are needed to correlate CMV genome detection in blood with presence of CMV antibodies.

Blood Donors↗
PubMed1984

Detection of cytomegaloviremia using monoclonal antibodies.

Isolation of cytomegalovirus (CMV) from blood plated on embryonic fibroblasts necessitates a delay of 10-30 days. Using monoclonal antibodies (McAb) we have developed a 96 hour test to diagnose viremia. We produced monoclonal antibodies directed against CMV (strain AD 169). Clone E 13 McAb detected a CMV early antigen in nuclei using indirect immunofluorescence. These monoclonal antibodies recognized reference strains (Davis and Towne) and 20 field isolates of CMV. E 13 McAb were therefore selected for the diagnostic tests. MRC 5 cells grown on coverslips in tubes were inoculated with buffy-coat from 51 blood samples from bone marrow allograft recipients and Guillain Barre polyneuritis, and centrifuged on hour at 4000 g at 36 degrees C. The indirect immunofluorescence test (IIF) was performed using E 13 McAb, 48 and 96 hours post infection. Three viremia were detected within 48 hours and five others within 96 hours. Plasma and buffy-coat were also inoculated onto MRC 5 cells grown in flasks. Flasks were observed daily for CMV cytopathic effect (CPE). CPE appeared within an average of 14 days. When CMV was detected by CPE, the IF tests were always positive within 96 hours after drawing the blood.

Antibodies, Monoclonal↗
PubMed1984

Use of monoclonal antibodies in the diagnosis of chlamydial urethritis and cervicitis.

Isolation of Chlamydia trachomatis (C. trachomatis) in cell culture is the most reliable method for the diagnosis of chlamydial infections. The results are obtained in 48-72 hours. We have produced anti-chlamydial monoclonal antibodies (McAb) and by means of indirect immunofluorescence (IIF) we have developed a rapid diagnostic test. Sixty-six patients, 34 males seen for subacute urethritis authenticated by the presence of polymorphonuclear cells on urethral smears and 32 females presenting with leucorrhea were included in the study. The specimens obtained from urethra and cervix were inoculated on Hela 229 cells for isolation of C. trachomatis. A similar specimen was placed in normal saline containing 2% fetal calf serum. 50 microliters of the saline solution were then placed in wells in IF slides, dried at 37 degrees C and fixed for 30 minutes in acetone at -20 degrees C. Anti-chlamydial McAb and fluorescein labelled mouse anti IgG were used for the rapid IIF test. From 34 cases, we have isolated C. trachomatis from the urethra of 19 males. In these 19 cases, most of the polymorphonuclear cells demonstrated a brilliant cytoplasma revealing the presence of chlamydial antigens. In 25 other cases without isolation, the rapid diagnostic test was positive twice. From the 32 females, C. trachomatis was isolated 7 times. In these 7 cases, the rapid diagnosis IIF test was positive with the same characteristics as noted in the male population. In the 25 other cases without isolation, the test was positive 3 times.

Animals↗
PubMed1983

[Herpesvirus infections during treatment inducing remission of acute leukemia].

Herpes virus hominis infection was observed in 28% (diagnosis ascertained on viral examination) to 33% (clinical suspicion) of 36 adult patients prospectively studied during remission induction chemotherapy for acute leukaemia. Symptoms are non specific so that diagnosis relies upon viral isolation. All infected patients but one survived the viral infection and the prognosis of the underlying acute leukaemia was unaffected.

Acute Disease↗
PubMed1983

[Non-gonococcal urethritis and cervicitis. Isolation of Chlamydia trachomatis and detection of anti-Chlamydia antibodies by indirect immunofluorescence].

The responsibility of Chlamydia trachomatis in non-gonococcal urethritis and cervicitis was investigated in 267 patients of both sexes. It was confirmed in 36.3% of patients with urethritis and 20.9% of patients with cervicitis by isolating C. trachomatis on Hela 229 cells in the presence of cytochalasin B. No clinical feature specific of C. trachomatis infection could be elicited. The patients were tested for total IgM-type serum anti-chlamydia antibodies by indirect immunofluorescence (IF), using as antigen the inclusions formed in Hela 229 cells by an L2 serotype of C. trachomatis. The serological study was also performed in 86 blood-donors used as controls. The diagnostic value of IF serology is limited in lower genito-urinary infections; the presence of specific IgM's correlates well with the isolation of C. trachomatis, but these IgM's are not detected in protracted urethritis or cervicitis. In such cases, the aetiological diagnosis can only be made by isolation of C. trachomatis from the focus of infection.

Adolescent↗
PubMed1983

[Herpes virus infections during a remission induction treatment in acute leukemias].

Herpes virus hominis infection was observed in 28% (diagnosis assessed on viral examination) to 33% (clinical suspicion) of 36 adult patients prospectively studied during remission induction chemotherapy for acute leukaemia. Symptoms are non specific so that diagnosis relies upon viral isolation. All infected patients but one survived the viral infection and the prognosis of the underlying acute leukaemia was unaffected.

Acute Disease↗
PubMed1983

[Production of monoclonal antibodies against human cytomegalovirus].

Cell fusion of Sp2/0, a murine myeloma derived non-secreting variant, with splenic lymphocytes from Mice immunized against human cytomegalovirus, has originated two stable hybridomas producing monoclonal antibodies (IgG) which react specifically with antigens that appear early in infectious cycle and remain localized in the cell nucleus.

Animals↗