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R Colimon

Publications and source records attributed to R Colimon.

40 records · Page 3Linked to original sources
PubMed1980

[Determination of IgM antibodies by complement-fixation after serum fractionation by sucrose density gradient in children and adults with evidence of Mycoplasma pneumoniae infection (author's transl)].

Sera from 45 patients with serologic evidence of Mycoplasma pneumoniae infection were fractionated by sucrose gradient centrifugation. The distribution of complement-fixing (CF) antibodies for Mycoplasma pneumoniae within the 19S and the 7S immunoglobulins was determined all patients below 20 years of age and 44% of older patients had 19S specific antibodies. A predominance of 19S relative to 7S CF antibodies was observed in 40% of cases below 20 years and in 18% of cases above 20 years. The meaning of these data is discussed.

Adolescent↗
PubMed1979

[A method for the preparation of a Chlamydiae group-specific antigen on hela-229 cells infected with a strain of "Chlamydia trachomatis" for use in the complement fixation test (author's transl)].

A simple method for the preparation of a potent group-specific antigen on HeLa-229 cells infected with MRC-1 (LB) (TRIC/GB/MRC-1 Gf) strain of Chlamydia trachomatis is outlined. HeLa-229 cells are infected (MRC-1 strain, 102 inclusion-forming units per cell) with centrifugation at 4,000 g for 1 h in flat-bottomed vials. The cells are removed by brief trypsinization with 0.25% trypsin and put into 75 cm2 culture flasks (12 x 106 cells by flask) in BHK-21 medium supplemented with foetal bovine serum. The flasks are incubated for 5 days at 37 degrees C. The destroyed cell monolayer and the supernatant are centrifuged at 100,000 g for 1 h. The pellet is collected and resuspended in PBS and subjected to ultrasonic vibration for 30 min. This antigen may be used for detection of complement-fixing antibodies in lymphogranuloma venereum and ornithosis.

Antigens, Bacterial↗