Biomedical subjects

R Crespo

Publications and source records attributed to R Crespo.

41 records · Page 3Linked to original sources

PubMedDate not supplied

Evaluation of different procedures for processing avian air sac lavage fluid.

Air sac lavage has become a routine diagnostic tool used in pet birds; however, cytologic techniques for collection and handling of avian cells have not been reported. In this study, directed endoscopy was used to obtain the air sac washes. Different factors were compared that might affect final preservation of cell morphology, such as centrifugation speed, anticoagulant, storage temperature, time to processing, addition of glutaraldehyde to the sample, and addition of protein. The goal was to find the processing technique best suited for the evaluation of cells from air sac washes. The use of an anticoagulant did not influence cell quality. Optimal cell morphology was achieved when the sample was centrifuged at a speed of 1000 rpm (89.4 X g) and when prepared in less than an hour from the time of collection. Low temperature (5 C) tended to preserve cell quality better. The addition of protein (fetal bovine serum) helped to preserve cell quality and was of value if the sample storage time was greater than 30 min. The addition of glutaraldehyde resulted in a significant reduction in cell quality.

Air Sacs↗

PubMedDate not supplied

Development of the thoracic air sacs of turkeys with age and rearing conditions.

Cytology and structure of the thoracic air sac of turkeys were investigated at four different ages (26-day embryo, 1 day, 2 wk, and 10 wk old) and two rearing conditions (isolation and commercial). Cytology was performed by guided fiberoptic endoscopy on the left thoracic air sac of each bird. The right thoracic air sac was sampled for light and electron microscopy. Heterophils were the most common nonepithelial cell found in air sac fluid. followed by macrophages and lymphocytes. Macrophages were most abundant in 1-day-old turkeys and turkeys raised in commercial conditions. The epithelium of the air sac consisted of squamous and cuboidal cells, with a few ciliated columnar and nonciliated columnar cells. Cuboidal cells had similar characteristics to type II pneumocytes. The mucociliary system was organized in tracts extended from the ostium to the posterior parts of the air sac. The number of ciliated tracts decreased with age, and the air sacs of commercial turkeys had a larger proportion of ciliated epithelium than did those of isolation birds. The epithelium may protect against disease by a structured mucociliary transport system, the production of surfactant, and phagocytosis of foreign particles. Differences in cytology and structure may reflect the maturation of the immune system and/or response to environment.

Aging↗

PubMedDate not supplied

Endoscopic gross anatomy findings of the thoracic air sac of the turkey.

The gross anatomy of the cranial thoracic air sacs was studied in the live turkey with a fiber-optic endoscope fitted with a video system. Thirty-five out of 39 (89.7%) of the turkeys had paired single thoracic air sacs with two ostia. The other 10.3% of the turkeys had paired cranial and caudal thoracic air sacs, each with a single ostium. The air flow pattern through the common thoracic air sac with two ostia is unknown. Furthermore, 42.3% of the turkeys with a single thoracic air sac had an invagination arising from the floor of the air sac that partially divided the single thoracic air sac creating a blind-ended sac. The blind sac formed by the invagination may trap small inhaled particulate matter.

Air Sacs↗

PubMedDate not supplied

Managing the development of dialysis nursing: ten years experience.

Different authors have stressed the need for specialised knowledge to serve the needs of the renal patient. This is due especially to the great advances in renal function substitution techniques which require training and technical, scientific and specific expertise on the part of the nursing staff who perform their work in renal units (1,2,3).

Curriculum↗

PubMedDate not supplied

Inclusion body tracheitis associated with avian adenovirus in turkeys.

Nine turkey flocks with basophilic intranuclear inclusion bodies, suggestive of adenovirus, within the epithelial cells of the tracheal mucosa were studied. Respiratory signs and increased mortality occurred in turkeys between 6 and 10 wk of age from nine commercial turkey meat flocks in central California. Necropsy findings included tracheitis and occasional mild sinusitis. Histopathology of the tracheas revealed epithelial deciliation, squamous metaplasia, large basophilic intranuclear inclusion bodies within epithelial cells, and lymphoplasmatic inflammation in the mucosa. Electron microscopy of the mucosa revealed hexagonal viral particles, approximately 73 nm in diameter, consistent with adenovirus within the nuclei of epithelial cells. All tracheal sections were negative for adenovirus group II by specific immunoperoxidase staining. Adenovirus group I was isolated from the trachea. In addition, Bordetella avium, Ornithobacterium rhinotracheale, and Klebsiella pneumoniae were isolated from the tracheas of three, three, and two flocks, respectively. Statistically greater total mortality and a smaller percentage of marketed turkeys were observed in the submitted flocks than in randomly selected flocks. No significant difference was observed between the two turkey groups in market weight, feed conversion, or percentage of grade "A" turkeys.

Adenoviridae Infections↗