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Biomedical subjects

R Hauser

Publications and source records attributed to R Hauser.

At least 163 records · Page 9Linked to original sources
PubMed1990

[Differentiation between in-life and post-mortem hemorrhage].

The determination of the time of haemorrhaging can be of substantial importance in the forensic practice. Histological, immunohistochemical and morphometric investigations were carried out on haemorrhages from animal experiments and from human pathological samples. Centrifugal topology, serum-erythrocyte discontinuity, topological arrangement of Fibrin formation and stabilisation of Factor XIII A were evaluated as criteria to define antemortal origin of haemorrhages.

Adipose Tissue↗
PubMed1989

[Immunochemical and chromatographic determination of ABH antigens in compact bone tissue].

The identification of ABH antigens from compact bone tissue is known from many sources. The purpose of this study was to make a contribution to the localization of blood-group-active substances in compact bone tissue. A variety of preparation and identification methods were successfully used and compared. Samples were extracted from compact bone tissue, separated by HPTLC, and examined using the absorption-elution and PAP techniques. Additionally, the PAP technique was carried out on cryostat sections. Serologically active blood-group substances were consistently demonstrated in the organic components of the haversian canals.

ABO Blood-Group System↗
PubMed1989

[Detection of glycosphingolipids of ABH and Le antigens on thin-layer plates using the PAP method].

Stroma from hemolyzed erythrocytes of blood groups 0, A1, B and A1B were obtained and subjected to butanol/phosphate buffer extraction. This extract was separated using HPTLC, and the ABH and Le substances were detected on the chromatogram using the PAP technique. The staining of the bands allowed specific demonstration of the serologically active glycosphingolipids present in the ABH and Le blood group substances. The antigens of the AB0 system showed a 3- to 12-band pattern. Each of the antigens Lea and Leb presented 3 bands. The slight differences in the levels of glycosphingolipids of equal chain lengths are probably due to differences in their chemical structures.

ABO Blood-Group System↗
PubMed1989

[Clinical use of click evoked oto-acoustic emissions at the Freiburg ENT clinic].

Click-evoked otoacoustic emissions appear to be an efficient way to assess cochlear function in infants and neonates. A new technique developed by Bray and Kemp (1987) enables data to be gathered under clinical conditions. A total of 310 children aged between one day and five years were examined in a realistic clinical situation: 204 of them were admitted to the authors' outpatient department for evaluation of a hearing impairment and 106 were special-care babies or normal newborns. Otoacoustic emissions were successfully tested in 244 of these 310 children at the first attempt after performing behavioural tests. A classification of the emissions by the coefficient of the cross-correlation function and the frequency response spectrum was used. Emissions were observed in 95% (n = 181 ears) of the three- to five-year-old children with a mean behavioural threshold better than 20 dB. In 82% (40/49) of the special-care babies and in 89% (51/57) of the normal newborns a successful emission recording was be obtained. Such findings suggest that this technique for detecting emissions may be useful in early diagnosis of hearing impairments, especially when combined with simple behavioural tests.

Acoustic Stimulation↗
PubMed1989

[Magnetic resonance tomography in preoperative analysis of chronic post-traumatic osteomyelitis].

Chronic osteomyelitis in 8 patients was studied preoperatively by magnetic resonance (MR) imaging and in part of them also by computed tomography (CT). Morphologic information about extraosseous and intraosseous changes was important for surgical planning. In long bones CT generally is superior delineating bone structures (sequestra and fragments of devitalized bone in the intramedullary cavity) and MR may be used as an adjunct for localizing extraosseous and intraosseous abscesses. In trabecular bones as the os calcis MR seems to be equivalent to CT.

Adult↗
PubMed1989

[Sniff-induced negative pressure--a cause for the development of middle ear diseases?].

The significance of "sniffing" causing tubal dysfunction and thus leading to the development of cholesteatoma is discussed. We examined 244 healthy ears (18% sniff-positive), 21 ears with a traumatic perforation of the tympanic membrane (10% sniff-positive), 46 ears with chronic mesotympanic otitis (17% sniff-positive) and 40 ears with cholesteatoma (60% sniff-positive). Two different methods were used: on intact tympanic membranes the pressure chamber impedance method described by Munker-Pedersen (1972), and for perforated tympanic membranes a pressure transducer. Our results show that: (1) "sniffing" can cause negative pressure in the middle ear space; (2) the traditional concept of opening failure is no longer sufficient to explain tubal dysfunction; (3) sniff-induced negative pressure is a further possible cause of tubal dysfunction; (4) sniff-induced negative pressure seems to be an important factor in the development of cholesteatoma.

Adolescent↗
PubMed1989

[Analysis of erythrocytic carrier molecules (glycosphingolipids) with ABO and Le specificity].

Membrane structures (stroma) were obtained from erythrocytes of various ABO and Le blood groups using a butanol/phosphate buffer extraction. The extracts were separated using thin layer chromatography and the ABO and Le antigens demonstrated directly on the chromatogram using the PAP-technique. Detection was carried out using either Ulex europaeus lectin or monoclonal antibodies. By this method the direct staining of the bands allowed a specific demonstration of the ABH and Leactive glycosphingolipids. A determination of the number of sugar residues is possible as well as the recognition of quantitative variants.

ABO Blood-Group System↗
PubMed1988

[Lung function and symptoms in employees of poultry farms].

Environmental measurements were made in different types of poultry confinement buildings. Levels of total dust 1.6 meters above the ground averaged 5.0 mg/m3. Ammonia levels averaged about 13 ppm with peak values of about 50 ppm. To study possible respiratory problems of the people working in these confinement buildings, the ventilatory lung function was measured and an interrogation concerning respiratory symptoms was carried out by 26 (7 females, 19 males) poultry farmers. A very high prevalence (10 of 26) of obstructive ventilatory lung function changes was found. The FEV1 values of the exposed men were significantly lower than the predicted normal values (ECCS). An association between duration of exposure and pulmonary function exists.

Adult↗
PubMed1988

The early development of Kraepelin's ideas on classification: a conceptual history.

Psychiatry still lives in a Kraepelinian world and scholarship on the work of Kraepelin has consequently been unhistorical. Selective exegesis of the various editions of his textbook has led to a rigid view of his contribution. However, Kraepelin lived and wrote during an important period of European intellectual history and his work can only be understood against this background. This paper analyses the development of his views in terms of the 'Research Programme' he planned early in his life and whose objective was the creation of a stable description and classification of the psychoses. This Kraepelin eventually achieved by longitudinally studying patient cohorts in terms of methodological criteria such as illness course and incurability. In the event, this methodology allowed him to identify by correlation 'clinical pictures' that both represented the 'essence' of the disease and provided a taxonomic criterion. Although avowedly atheoretical, Kraepelin thus managed to construct (influenced by Kahlbaum and Wundt) an empirical support for his Kantian categorization of the psychoses. A discussion of the cultural variables that moulded these ideas is included.

Germany↗
PubMed1987

Evidence for partial exocrine acinar differentiation in experimentally induced pancreatic ductal/ductular cell tumors.

The pancreatic carcinomas induced in Syrian hamsters by a nitroso compound are of a ductal/ductular cell type. Electron microscopic examination of such tumors has revealed the presence of an occasional isolated acinar cell within the tumor. Based on their cellular characteristics, these acinar cells seem to have derived from ductular cell precursors. When we used a monoclonal antibody which specifically recognizes hamster acinar cells, a larger number of cells bearing acinar cell membrane markers were found in many tumors by an indirect immunofluorescence technique. Since most of these positively reacting cells did not exhibit the characteristic cytological features of acinar cells, such as rough endoplasmic reticulum and zymogen granules, differentiation of precursor cells to acinar cells may possibly occur at the cell membrane level. The presence of a few acinar cells within exocrine pancreatic tumors implies that acinar differentiation or retrodifferentiation can occur in pancreatic neoplasms.

Adenocarcinoma↗
PubMed1987

[Fatty liver in indirect calorimetry controlled total parenteral nutrition].

The authors have investigated the relationship between total parenteral nutrition (TPN) under indirect calorimetry and the development of fatty infiltrations of the liver in 21 surgical and traumatized patients. TPN was monitored by indirect calorimetry in order to balance lipid and amino acid intake. Glucose intake was adapted only when daily glucose utilization was higher than 150 g. Non protein respiratory quotient (npRQ) and respiratory quotient (RQ) remained below 0.9 during the whole study. The need for lipids was always significantly higher than the lipid intake up to the 5th day after operation or trauma. The need for carbohydrates was significantly lower than the intake of carbohydrates up to the 9th day after operation or trauma. In addition, the utilization of lipids was higher than the utilization of carbohydrates. After 2 weeks of TPN, no fatty infiltration of the liver could be detected by computer tomography and chemical analysis. The calorimetric monitoring of the TPN regimen and the individual adaptation of substrate intake might be responsible for the protection of the liver observed in this study.

Blood Glucose↗
PubMed1986

[Determination of the blood group properties of bones subjected to the decay process].

The aim of the present study was to make generalizations concerning the results of serological examinations of bones subjected to the process of decay. The specimens examined were taken from the femur. Some of the specimens had been subjected to decay for a period of some years, after which they were examined using the absorption-elution method in accordance with classical principles. Finally, successive dilutions of eluted antibodies were performed with a view to establishing the "antigenic force" of the material under examination. It was found that, owing to the process of bone decay, there is a decrease in the original "antigenic force", which is accompanied by the appearance (in a weak form) of non-specific serological reactions, which are much weaker in strength than specific ones. It was concluded that for the interpretation of the actual blood group of decayed bones the term "diagnostic" can only be applied to a range of dilutions not exceeding that of non-specific agglutinations.

ABO Blood-Group System↗
PubMed1986

Nephrotoxicity of cyclosporine in spontaneously hypertensive rats: effects on blood pressure and vascular lesions.

Cyclosporine (CSA) caused functional and morphologic renal changes in spontaneously hypertensive rats. The development of hypertensive arterial lesions were accelerated by CSA. Blood pressure was significantly increased especially in young rats. The influence of age of the animals, dose and duration of CSA administration on the development of vascular lesions and blood pressure was investigated.

Animals↗
PubMed1985

Interference of cyclosporin with lymphocyte proliferation: effects on mitochondria and lysosomes of cyclosporin-sensitive or -resistant cell clones.

Cyclosporin was previously shown to interfere with--but not to abolish--the increased activities of lysosomes and mitochondria consequent to a mitogenic activation of normal mouse lymphocytes. This was evident from the fluorescence profiles of cell populations after vital staining with euchrysine (giving a lysosomal-specific red fluorescence) and rhodamine-123 (giving a mitochondrial-specific green fluorescence). Fluorescence profiles of the population of cells not exposed to a mitogen were also altered by cyclosporin, with lower lysosomal and mitochondrial fluorescence of these cell populations. In order to find out more precisely what could be the direct effects of cyclosporin on those cellular organelles, our cyclosporin-sensitive (BE7) and cyclosporin-resistant (LB7) lymphoblastoid cell lines were tested and showed clear-cut differences. Only minor effects could be detected for the lysosomal and mitochondrial activities of the resistant cells. On the contrary, cyclosporin caused, in the cells of the sensitive clone BE7, a clear decrease of mitochondrial activity together with an unexpected increase of the red fluorescence of euchrysine. The latter might not correspond to a real increase of the lysosomal activity of such cells. Indeed electron microscopy studies do not show higher numbers of lysosomes; rather they show that numerous vacuoles appear in the cytoplasm of the cyclosporin-treated BE7 cells (but not in the cells of the resistant clone and not in untreated cells of either types).

Animals↗