[Indications and results of lateral and median episiotomy].
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Biomedical subjects
Publications and source records attributed to R Ivanova.
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Alkali-treated lipid A prepared by hydrolysis of lipid A in 0.25 N NaOH (1 h, 56 degrees C) formed an insoluble complex with methylated bovine serum albumin (MBSA) in aqueous medium. At a 1/1 (w/w) lipid A/MBSA ratio, nearly all lipid A was insolubilized. The complex was assumed to be ionic in nature. On intravenous immunization of rabbits with the complex, antibodies directed against lipid A were elicited. The antibody titres ranged from 1/256 to 1/2,048, as demonstrated by the passive haemolysis test, and proved to be mainly of the IgM class.
Antiserum to the epimeraseless Rc mutant of Salmonella minnesota was prepared by immunization of rabbits. The antiserum provided good protection in mice i.p. challenged with 125 and 25 LD50 doses of live heterologous Gram-negative bacteria i. e. S. typhimurium, Escherichia coli and Pseudomonas aeruginosa. The protection against Klebsiella pneumoniae was negligible. Pre-immune rabbit serum produced only marginal protection. The protective effect of S. minnesota Rc antibodies was entirely abolished upon absorption of Rc antiserum with homologous bacteria. Pre-immune and absorbed sera exhibited a similar protective effect at lower LD50 values, namely 5 and 1 LD50.