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R K Gupta

Publications and source records attributed to R K Gupta.

At least 937 records · Page 52Linked to original sources
PubMedDate not supplied

Fine catheter aspiration cytology of the peritoneal cavity in the diagnosis of a metastatic tumor. Report of a case with immunocytochemical studies.

The potential value of a new system of fine catheter aspiration (FCA) cytology of the peritoneal cavity in the diagnosis of peritoneal malignancy is illustrated by a case report. Gelman filter preparations of an FCA sample of an abdominal mass from a patient with a history of adenocarcinoma of the lung showed unequivocal malignant cells, obviating the need for further diagnostic procedures. Trial immunostaining of Cytospin preparations of part of the FCA sample showed appropriate results, suggesting that such samples may be suitable for immunoperoxidase studies to identify tumor types or to predict the source of the primary tumor in difficult cases.

Adenocarcinoma↗
PubMedDate not supplied

The value of needle aspiration cytology of the breast, with an emphasis on the diagnosis of breast disease in young women below the age of 30.

Our experience with 4,739 needle aspirations of the breast was reviewed, with an emphasis on the diagnosis of carcinoma in young women under the age of 30 years. Among benign diseases, fibroadenomas were most frequently seen in younger women (24.3% of 919 cases) while cysts were most frequently seen in women 30 years of age and older (20.0%). Fibrocystic conditions were identified in approximately 30% of the women in each group. All younger women with suspicious diagnoses had fibrocystic disease at biopsy; in older women, 75% of the suspicious cases proved to be carcinomas while 25% proved to be fibrocystic diseases. Carcinomas were diagnosed cytologically in 1.3% of younger women and in 9.7% of older women. In young women, in whom breast carcinoma is a rare disorder, it is important that carcinoma be diagnosed without delay so that an appropriate plan of management can be adopted. These findings suggest that needle aspiration cytology can accurately make that diagnosis.

Adolescent↗
PubMedDate not supplied

Immunocytochemical and ultrastructural study of the rare osteoclast-type carcinoma of the breast in a fine needle aspirate.

The cytologic findings in a fine needle aspirate from a case of the rare osteoclastlike carcinoma of the breast are described along with the immunocytochemical and ultrastructural findings. The ultrastructural and immunocytochemical findings suggest that the osteoclastlike giant cells in this type of carcinoma are not of epithelial origin, but rather are reactive and of a histiocytic-stromal origin.

Adult↗
PubMedDate not supplied

Effect of metoclopramide on peristalsis and antiperistalsis in isolated frog stomach and guinea-pig ileum.

The action of metoclopramide was studied on peristaltic as well as antiperistaltic activity in frog stomach and guinea-pig ileum. It was observed that metoclopramide enhances normal peristaltic activity by acting on dopamine-receptors whereas it inhibits antiperistalsis by some unknown mechanism. It is, therefore, inferred that inhibition of antiperistaltic activity might be an additional mechanism for its antiemetic effect. It also explains the pharmacological basis for its use in oesophagitis due to gastro-oesophageal reflux.

Animals↗
PubMedDate not supplied

Diagnosis of unsuspected pulmonary cryptococcosis with sputum cytology.

This paper describes our experience in the sputum cytology diagnosis of pulmonary cryptococcosis, the clinical diagnosis of which is usually difficult. The cytologic findings are described briefly along with other pertinent supportive data, and the importance of sputum cytology in the diagnosis of this clinically occult mycotic disease is emphasized. The cytologic examination of deep cough samples of sputum in cases with an appropriate clinical setting and pulmonary infiltrates not only can be a rewarding procedure but also can result in the institution of an appropriate line of treatment for the possible prevention of widespread dissemination.

Adult↗
PubMedDate not supplied

23Na, 19F, 35Cl and 31P multinuclear nuclear magnetic resonance studies of perfused rat kidney.

The concentration of intracellular sodium [Na+]i has been measured in the perfused rat kidney using 23Na nuclear magnetic resonance (NMR) in combination with the extracellular shift reagent Dy(PPPi)7-(2). The data show 100% visibility of Na+ in interstitial spaces. A measurement of the resonance intensities of intra- and extracellular 23Na ions along with a knowledge of the extracellular space as a fraction of the total kidney water space yielded an average [Na+]i of 27 +/- 2 mM for the kidney at 37 degrees C. After prolonged ischemia [Na+]i rose to approach that in the external medium. In the absence of 5% albumin in the perfusion medium, the linewidth of the 35Cl resonance of an adult kidney (45 Hz) was about twofold larger than that of the medium alone (25 Hz). In contrast, the linewidth of 35Cl resonance of an adult kidney perfused with an albumin-containing medium (82 Hz) was only about 27% of that from the medium alone (300 Hz). We interpret this effect to be due to compartmentation of albumin in the extracellular space such that the interstitial space is not freely accessible to albumin. However, for a developing, immature kidney from a growing animal, perfused with an albumin-containing medium, the linewidth of the 35Cl resonance (233 Hz) was only slightly less than that of the medium alone (300 Hz), indicating a much greater albumin permeability of the capillary walls. 19F NMR of a perfused adult kidney, loaded with the membrane-impermeant intracellular calcium indicator 5FBAPTA, yielded a value of 256 nM for [Ca2+]i. Induction of ischemia for 10 min caused the [Ca2+]i to rapidly rise to 660 nM, which could not be fully reversed by reperfusion, suggesting irreversible injury.

Adenosine Triphosphate↗
PubMedDate not supplied

Immunocytochemical and ultrastructural diagnosis of a rare mixed apocrine-medullary carcinoma of the breast in a fine needle aspirate.

A rare mixed apocrine-medullary mammary carcinoma in a 57-year-old woman was preoperatively diagnosed by fine needle aspiration cytology. The aspirate was characterized by carcinoma cells with an apocrine differentiation and significant cellular atypia admixed with many lymphocytes and plasma cells. These findings were confirmed by histologic examination of the breast tumor and its metastasis in lymph nodes. Electron microscopy (EM) and immunoperoxidase staining for cytokeratin, S-100 protein, epithelial membrane antigen and carcinoembryonic antigen were done on samples of the aspirated material. Although immunostaining was of little help in this case, the EM findings did show many carcinomatous cells of apocrine type in the tumor.

Biopsy, Needle↗
PubMedDate not supplied

Immunocytochemical and ultrastructural findings in a case of rare carcinoma of the pancreas with predominance of malignant squamous cells in an intraoperative needle aspirate.

The cytologic diagnosis by intraoperative needle aspiration of a rare carcinoma of the pancreas composed predominantly of malignant squamous cells is reported. Both poorly differentiated and keratinized well-differentiated squamous cells were seen in the aspirate. Tissue for histologic confirmation was not obtained because of the position of the mass. Confirmatory ultrastructural and immunoperoxidase studies were thus done on the aspirated material to confirm the diagnosis.

Aged↗
PubMedDate not supplied

Effect of metoclopramide on gastric ulceration and secretion in albino rats.

The antiulcer and antisecretory effects of metoclopramide were studied in aspirin-induced and pylorus-ligated gastric ulcers. The effects were compared with ranitidine (10 mg/kg, orally). The effect of propantheline alone and in combination with metoclopramide was studied against aspirin-induced gastric ulcers only. Metoclopramide (20 mg and 50 mg/kg) produced an ulcer protective effect both in aspirin-induced and pylorus-ligated experimental models. Its ulcer protective effect against aspirin-induced and pylorus-ligated gastric ulcers was comparable to ranitidine. Metoclopramide reduced the volume of gastric secretion but did not alter the total acidity; however, significant reduction in free acidity was noted. The ulcer protective effect of metoclopramide appeared to have multiple mechanisms like reduction in gastric secretion and free acidity, increased gastric emptying and cytoprotective action.

Animals↗
PubMedDate not supplied

Diagnostic value of ultrasonography in hydatid disease in abdomen and chest.

A prospective diagnosis of hydatid cyst was made in 90 cases involving abdomen and chest. It was based on the various sonographic signs of hydatid disease described in the literature. Surgery was performed in 60 patients while 30 were lost to follow-up. The diagnosis was confirmed in 55 patients while it was false positive in 5. The liver was the most common organ involved, followed by chest, kidney, peritoneum, spleen and retroperitoneum. Separation of the membrane from the wall, multiple daughter cysts, and collapsed cysts, were found to be diagnostic of hydatid disease. It is stressed that certain signs are specific for hydatid disease and thus do not cause any differential diagnostic problems.

Abdomen↗