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R Kaneko

Publications and source records attributed to R Kaneko.

76 records · Page 5Linked to original sources
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Hsp40, a possible indicator for thermotolerance of murine tumour in vivo.

The relationship between Hsp40/Hsp70 synthesis and the development of thermotolerance was investigated using mouse squamous cell carcinoma in vivo. To examine the thermotolerance, tumours were heated at 44 degrees C for 30 min as conditioning heating. After various intervals they were heated again at 44 degrees C for 90 min as challenge heating. The tumour response to heat was evaluated by the growth delay. Thermotolerance rapidly developed with increasing interval and reached a maximum at 12 h interval. Subsequently, thermotolerance gradually decayed and almost disappeared at 120 h interval. Under this condition, synthesis of Hsp40/Hsp70 increased after conditioning heating, reached a maximum at 12 h interval, then gradually decreased thereafter within 120 h. The kinetics of accumulation and decay of both Hsp40 and Hsp70 were very similar. The extent of thermotolerance was well correlated with the relative amount of Hsp40/Hsp70. These results obtained in vivo were very similar to those in vitro (Kaneko et al. 1995). Our findings suggest that Hsp40 could be a useful indicator of the degree of thermotolerance in addition to Hsp70 in vivo as in vitro.

Animals↗
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Impaired production of Th1 cytokines and increased frequency of Th2 subsets in PBMC from advanced cancer patients.

BACKGROUND: A reported imbalance of Th1 and Th2 may be responsible for the occurrence and progress of certain diseases. Patients with advanced cancer may have impaired cell-mediated immunity caused by a switch from Th1 to Th2. We investigated the balance of Th1 and Th2 in cancer patients using two approaches. METHODS: Flow cytometry was used to define Th1 and Th2 using intracellular cytokines, thereby determining the frequency of these helper T cells in CD4+ peripheral blood mononuclear cells (PBMC). The amount of cytokines secreted by stimulated PBMC in bulk culture was determined. RESULTS: Production of TNF-alpha and IFN-gamma in the culture was significantly lower in 9 patients (1188 +/- 634 pg/ml and 46 +/- 84 pg/ml) than in 10 healthy subjects (2491 +/- 1037 pg/ml and 295 +/- 219 pg/ml) (p < 0.002 and p < 0.003), while the production of IL-4 was slightly higher in cancer patients than in the healthy subjects. The percentage of IFN-gamma-IL-4+ cells in CD4+ cells was significantly higher in the patients than in healthy subjects (4.3 +/- 2.0% and 2.4 +/- 0.7%); there was no difference in IFN-gamma+IL-4- cells between the two groups. The ratio of IFN-gamma+IL-4- and IFN-gamma-IL-4+ cells per individual was significantly lower in the patients. CONCLUSION: These results indicated that an imbalance of Th1 and Th2 was found not only in the frequency of the subsets in PBMC, but also in the capacity for cytokine-production.

Case-Control Studies↗