Biomedical subjects

R Karlícek

Publications and source records attributed to R Karlícek.

11 recordsLinked to original sources

PubMed2001

[Sorbents for solid phase extraction and their use].

Extraction on the solid phase is a rapid, simple, effective, and flexible method of preparation of samples for the analysis proper and it can be used in many areas of analysis (pharmacy, biochemistry, food industry, environmental examination, etc.). Possible use in on-line and automatic systems is also advantageous. A very wide scale of proposed sorbents and the use of their combinations thus make extraction on the solid phase an effective method for the isolation and preconcentration of the analyte from the complex matrix of the sample.

Absorption↗

PubMed2000

[Oxidative determination of hydroxyamphetamine in drops using flow injection analysis].

A flow injection-spectrophotometric determination at 540 nm of hydroxyamphetamine is reported. The procedure is based on a coloured reaction with 2-hydrazono-3-methylbenzothiazole (MBTH) and ceric ammonium nitrate (CeIV) as the oxidation reagent. Under optimised conditions, the determination of hydroxyamphetamine is achieved in a range of 0.01-0.2 mmol.l-1 with a relative standard deviation of 0.78% for 0.05 mmol.l-1 (n = 10) and a sample throughput of 75.h-1. The method was used for the determination of medicinal drug in the mass-produced dosage form of Czech origin: Pedrolon drops, Galena a.c.

Flow Injection Analysis↗

PubMed1998

Unidirectional transfer of D-xylose across the rat placenta.

1. The transplacental transfer of D-xylose was investigated in the present study. 2. Umbilical perfusion of the rat term placenta was used to study materno-foetal (M-F) and foetomaternal (F-M) transfer of this compound. 3. D-Xylose was found to cross the rat term placenta very quickly in the M-F direction, with subsequent accumulation in the foetal compartment (the F-M ratio being 2.23 at the end of the experiments). In contrast, no transfer in the F-M direction was observed. 4. A possible facilitation of the transplacental transfer of D-xylose from the mother to foetus in rats is suggested.

Animals↗

PubMed1994

Increased urinary nitrate excretion in inflammatory bowel disease.

Urinary nitrate is known to be a measure of environmental exposure. Only recently an endogenous metabolic pathway resulting in nitrate synthesis was described. We have determined the concentration of nitrate expressed as nitrate/creatinine ratio in the urine of patients in whom a significant environmental nitrate load was presumed to be absent. A significant elevation of nitrates in patients with inflammatory bowel disease compared with patients with non-inflammatory disorders was observed (0.173 +/- 0.155 vs. 0.037 +/- 0.016 mol/mol creatinine, P < 0.01). Increased nitrate excretion in the urine of patients with inflammatory bowel disease may reflect the endogenous nitrate synthesis induced by the immune activation.

Adolescent↗

PubMed1984