Biomedical subjects
R Lindgren
Publications and source records attributed to R Lindgren.
Quantitation of serum tocopherols by high-performance liquid chromatography with fluorescence detection.
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Purification of a bovine counterpart to human prealbumin and its interaction with a bovine retinol-binding protein.
A bovine counterpart to human prealbumin was purified from bovine serum by thiol-disulfide exchange chromatography on thiol-Sepharose 4B and affinity chromatography on human retinol-binding protein linked to Sepharose 4B. The bovine prealbumin had alpha1-mobility on agarose gel electrophoresis at pH 8.6. It has the same molecular weight as human prealbumin on gel filtration and consisted of subunits with a molecular weight of 12 500. This is compatible with a tetrameric structure for the bovine protein. Antiserum against human prealbumin cross-reacted with bovine prealbumin and vice versa. The bovine prealbumin formed at high ionic strength complexes with another bovine serum protein which were dissociated at low ionic strength. This property was used to isolate a protein from bovine serum, by chromatography on bovine prealbumin linked to Sepharose which cross-reacted with antiserum against human retinol-binding protein; had a molecular weight of 21 000 and alpha 2-mobility on agarose gel electrophoresis. It was concluded that the latter protein was a bovine retinol-binding protein.
Antibodies to Chlamydia trachomatis in women with infertility, pelvic inflammatory disease and ectopic pregnancy.
Sixty-three women admitted to the gynecological ward at the University Hospital, Linköping, were studied for the presence of antibodies to Chlamydia trachomatis. 48% of infertile women, 78% of women with ectopic pregnancy and 44% of women with pelvic inflammatory disease had higher titer (greater than or equal to 1:128) IgG/IgA/IgM antibodies to C. trachomatis. Among 55 healthy pregnant women, used as controls, 13% had this high antibody titer. Among 30 women with a titer of greater than or equal to 1:128, 33% of previous conceptions resulted in ectopic pregnancy and 31% resulted in child birth. The corresponding figures among women with no antibodies to C. trachomatis were 12% and 60%, respectively. The prevalence of IgA antibodies varied from 22% to 38% in women with pelvic inflammatory disease, ectopic pregnancy or infertility. The results indicate the importance for C. trachomatis in development of sequelae to lower genital tract infections in women.