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Biomedical subjects

R M Gopinath

Publications and source records attributed to R M Gopinath.

3 recordsLinked to original sources
PubMed1985

Clinical training program for staff pharmacists.

This study describes the development and evaluation of a clinical training program in the areas of pharmacokinetics and infectious diseases for staff pharmacists in a decentralized unit dose drug distribution system. Five staff pharmacists were trained and three other pharmacists served as controls. The teaching sessions consisted of eight 1-hour lectures per group of two to three pharmacists. The lectures were supplemented by handouts, reading assignments, and practical assignments. There was a significant improvement in posttest scores of the trained pharmacists when compared to their pretest scores and from the scores of the control pharmacists. In the Seattle VA Medical Center pharmacy, this program was shown to be successful, and a similar program can be used to train staff pharmacists in other clinical areas of interest.

Cross Infection↗
PubMed1979

(Ca2++Mg2+)-ATPase activity of sickle cell membranes: decreased activation by red blood cell cytoplasmic activator.

Human red blood cells (RBCs) contain a cytoplasmic protein that activates membrane-bound (Ca2+ + Mg2+)-ATPase and the transport of Ca2+. The (Ca2+ + Mg2+)-ATPase of sickle cells showed a less than normal response to this activator. This was true whether the activator was obtained from normal or sickle cells. Activator present in sickle cell hemolysates fully activated the (Ca2+ + Mg2+)-ATPase of normal RBC membranes. These results demonstrate that membranes of sickle cells are defective in their response to the activator. Neither the apparent affinity for calcium nor the apparent affinity for activator was different comparing the (Ca2+ + Mg2+)-ATPase of sickle and normal membranes. Young, mature, and irreversibly sickled cells were separated by density gradient centrifugation, and membranes were prepared from each of these cell populations. No significant differences in ATPase activities were found based on cell age (density). The (Ca2+ + Mg2+)-ATPase of all populations of sickle cells showed a decreased response to the activator. Thus, it appears unlikely that the decreased response of the (Ca2+ + Mg2+)-ATPase of sickle cells is due to membrane damage caused by repeated sickling during the life-span of the cell. Reduced activation of (Ca2+ + Mg2+)-ATPase by the cytoplasmic activator may account for calcium accumulation in sickle cells.

Adenosine Triphosphatases↗