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Biomedical subjects

R M Mackie

Publications and source records attributed to R M Mackie.

At least 91 records · Page 5Linked to original sources
PubMed1980

Studies in cutaneous immunofluorescence. I. The effect of storage time on direct immunofluorescence of skin biopsies from bullous disease and lupus erythematosus.

Forty-eight biopsy samples submitted to a diagnostic immunofluorescence service have been stored at -20 degrees C and sequentially re-tested at monthly intervals to ascertain the duration for which immunoglobulins and complement can be identified in these samples in typical and diagnostic patterns of distribution. Four biopsies from pemphigus vulgaris, 14 biopsies from bullous pemphigoid, nine biopsies from dermatitis herpetiformis and 21 from chronic discoid lupus erythematosus have been studied in this manner. In all cases diagnostic immunofluorescence patterns were present up to 4 months after biopsy and in several biopsies a diagnostic pattern was observed at 9 months. It is concluded that adequate storage facilities for a diagnostic immunfluorescence service can be achieved with a -20 degrees C storage system and that known positive material can be retrieved from this system for use as controls or in research studies for a minimum of 4 months.

Biopsy↗
PubMed1979

Indirect leukocyte migration assay in patients with malignant melanoma.

Leukocytes from 72 melanoma patients and 75 control donors were examined in a two-stage (indirect) leukocyte migration assay, using formalin-fixed melanoma cells (FMC) and control, normal or non-melanoma tumour cells (FCC) as the source of antigen. Inhibitory supernatants were produced significantly more often by cultures of melanoma leukocytes and FMC than by melanoma leukocytes and FCC or control leukocytes and FMC or FCC. Reactive leukocytes were most frequently derived from stage II patients (65%), followed by stage I patients with tumour present (58%), stage III patients (29%) and stage I patients without detectable tumour (20%). The likelihood that a melanoma patient's leukocytes would react increased progressively with the number of different FMC preparations tested. A similar, though less steep increase in reactivity was seen with control donor leukocytes and different FMC preparations. No significant increase in reaction frequency was seen with melanoma or control leukocytes exposed to increasing numbers of different FCC preparations. Significant discrimination between melanoma patients' and control donors' leukocytes was achieved with FMC from both primary and metastatic tumours. Concordance of positivity or negativity was seen in 60% of concurrent one-stage and two-stage leukocyte migration assays.

Adult↗
PubMed1978

Wiskott-Aldrich syndrome with partial response to transfer factor.

A male infant presented with dermatitis, purpura and susceptibility to bacterial infections. The clinical diagnosis of Wiskott-Aldrich syndrome was confirmed and after full immunological assessment, treatment with transfer factor was commenced. This has resulted in a rise in the platelet count and improvement in the bleeding tendency. This improvement in the haematological aspect of the disease has, however, been accompanied by exacerbations of the cutaneous lesions.

Blood Cell Count↗
PubMed1978

The relationship between immunological parameters and response to therapy in resistant oral candidosis.

Eight patients with resistant oral candidal infection have undergone full immunological assessment. In four of these patients no significant abnormality of immune function was observed, and all responded to intensive antifungal therapy with nutritional supplements of iron and folic acids as required. The remaining four patients had abnormalities only of specific candida-related immune function and no detectable abnormality of general immune function. Surgical excision of affected areas of oral mucosa was found to be the most effective therapy in three of these four cases. The immunological findings in these cases are compared with those observed in five patients with chronic mucocutaneous candidosis in whom both specific candida-related and non-specific immune incompetence were present.

Adult↗
PubMed1977

Evaluation and staging in the Scottish Dermatological Society mycosis fungoides register.

The patients registered in the first two years of the Scottish Dermatological Society's Malignant Lymphoid Neoplasm Register are described. Criteria for inclusion in the Register and laboratory tests carried out in the staging of these patients is listed. Significant abnormalities detected to date include low levels of circulating E and EAC rosette forming cells and an elevated IgE level in a proportion of patients. It is hoped that continued monitoring of these patient will clarify the relationship of these abnormalities to the natural progression of the disease.

Humans↗
PubMed1976

Leukocyte migration inhibition by cancer patients' sera.

Sera from 38 of 89 melanoma patients, 4 of 15 patients with other cancers and 3 of 43 control donors inhibited the migration of autologous leukocytes. Sera from patients with clinically detectable metastatic disease and from the recipients of BCG were most frequently inhibitory. Autologous sera from 31 of 71 melanoma patients and 3 of 31 control donors increased the leukocyte migration inhibition induced by formalinized melanoma cells. This activity occurred in sera from patients at all stages of malignancy, whether bearing detectable tumour or not and whether receiving BCG or not. Abrogation of tumour-cell-induced leukocyte migration inhibition by serum was less frequent, occurring with 8 of 71 melanoma patients (6 Stage III, 1 Stage I and 1 Stage II) and no control sera. Preincubation of formalinized tumour cells had no effect on their activity in the leukocyte migration assay but the preincubation of melanoma leukocytes with either autologous serum or foetal calf serum inhibited leukocyte reactivity in around 50% of tests. However, this was also seen with control leukocytes and the effect appears to be immunologically non-specific.

Animals↗
PubMed1976

An examination of the immunology of cancer patients.

We have examined 111 cancer patients and 111 control individuals for general immunocompetence (haematological values, "recall" antigen skin tests, PHA and PPD induced lymphocyte transformation, serum Ig levels and lymphocyte subpopulations), for evidence of sensitisation to tumour-associated antigens (leucocyte migration test, serum inhibition of autologous leucocyte migration, lymphocytotoxicity, membrane immunofluorescence and immune adherence) and for evidence of continuing immune reactions (alterations of complement components and anticomplementary activity). Major differences between the cancer patients and controls were demonstrated by several tests of sensitisation and these also detected differences between patients with and without metastases. The only differences detected between cancer patients and controls by the tests of general immunocompetence were in serum IgG and IgA (higher in the cancer patients) and lymphocyte subpopulations ("active" T, autorosetting lymphocytes and lymphocytes forming "super-rosettes" increased in cancer patients). In a comparison of cancer patients with and without metastases, patients with metastases were less often reactive to the Candida DHS and streptokinase-streptodornase antigens and had raised circulating Fc positive cells. Abnormalities of the individual components of complement occurred in about half the cancer patients, but were equally common in those with and without metastases. Serum anti-complementary activity was very rarely detected. The tests of specific sensitisation correlated reasonably well but correlations of tests of general immunocompetence were infrequent.

Adolescent↗
PubMed1975

Serum anti-tumour antibodies and auto-antibodies in vitiligo.

Anti-tumour antibody was sought in the sera of three groups of patients; idiopathic vitiligo, malignant melanoma and miscellaneous skin disorders. No precipitating antibody to a series of prepared pigmented tumour antigens was demonstrated. All sera were tested for a number of auto-antibodies. No significant differences were detected between the groups. The evidence for an immunological factor in the causation of vitiligo remains circumstantial only.

Adolescent↗
PubMed1975

Formalinized tumour cells in the leucocyte migration inhibition test.

Immunization studies indicate that formalinized tumour cells retain at least part of their gross membrane structure and antigenicity; they are relatively easy to prepare and store well over a period of months. We have used formalinized tumour cells as antigen in the leucocyte migration inhibition test. Leucocyte migration inhibition occurred in leucocytes from thirty-eight out of sixty-nine malignant melanoma patients and from fifty-five out of eight-one breast cancer patients when in contact with formalinized tumour cells of a histologically similar type. Melanoma patients' and breast cancer patients' leucocytes were infrequently inhibited on contact with cells from histologically dissimilar human tumours and from xenogeneic mouse melanomas. Other advantages of formalinized cells over antigens prepared by homogenizing tumour tissue include a greater degree of inhibition and the ability to demonstrate a dose-response relationship between the ratio of leucocytes:tumour cells and the migration index.

Antigens, Neoplasm↗