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R Maciejewski

Publications and source records attributed to R Maciejewski.

At least 37 records · Page 2Linked to original sources
PubMed1999

Changes in the activity of some lysosomal enzymes and in the fine structure of submandibular gland due to experimental diabetes.

The aim of this study was to establish and quantify changes in the activities of the some lysosomal enzymes and to determine the type of changes in the ultrastructure of the submandibular gland in rabbits caused during progression of diabetes. The experiment was conducted on 89 New Zealand rabbit males. Diabetes was induced by the intravenous administration of 10% alloxan solution at a dose of 10-mg/kg-body weight. On the seventh day after alloxan administration, the level of glucose in blood was determined. Rabbits were divided into five groups: intact (n=18), 21-day diabetes (n=18), 42-day diabetes (n=17), 90-day diabetes (n=19) and 180-day diabetes (n=17). From killed animals in each group, the submandibular glands were removed and fixed or stored. Enzyme activities were assayed by spectrophotometric methods using substrates (Sigma) which release 4-methyloumbeliferol when they react with the proteases. Fixation procedure was done according to standard methods. Semi-thin and ultra-thin specimens were prepared by use of clearly visible after 42 days of diabetes. Mitochondria were damaged, accumulation of large amounts of lipids in the intracellular spaces was observed. After 90 days the presence of vacuoli and swollen lysosomes were observed, some cells also contained myelin figures. After 180 days the greatest changes were observed in the blood vessels, which had thickened walls and were often occluded. We concluded that the total activity of acid phosphatase and beta-N-acetyl-glucosaminidase in the submandibular gland was correlated with the level of glucose but there was no correlation between total beta-galactosidase activity and the serum concentration level of glucose has been detected during course of diabetes. The activities of the free fractions of acid phosphatase, beta-galactosidase and beta-N-acetyl-glucosaminidase in the submandibular gland were higher than the bound fractions in all groups of rabbits. The changes in the ultrastructure of the submandibular gland were correlated with changes in serum glucose level and with lysosomal enzymes activities during progression of experimental diabetes in rabbits.

Acid Phosphatase↗
PubMed1998

Branches of posterior cerebral artery vascularising visual cortex in man.

In 180 brains it was found that the calcarine artery was the main branch supplying the medial surface of the occipital lobe. Also the accessory calcarine artery contributed to vascularization of the occipital lobe. This artery originated from the medial occipital, lateral occipital, parietooccipital, and occipitotemporal arteries.

Adolescent↗
PubMed1997

[The effect of intraperitoneally administered necrolytic enzymes on the course of experimental acute pancreatitis].

The acute pancreatitis is still serious diagnostic and therapeutic problem. In spite of many experiments there are no satisfactory methods of the treatment of this disease. Usually, it is diagnosed clinically by acute abdominal pain and increased pancreatic enzymes in blood and urine. The main disorder of this disease is the necrosis of the pancreatic gland. The aim of our investigations was the evaluation of effect of necrolytic enzymes on the course of acute experimental pancreatitis. Fibrolan was used. Fibrolan (Parke-Davis) is a preparation, which has necrolytic properties. It consists of two bovine enzymes: fibrinolysin and deoxyribonuclease. The examinations were carried out on 380 Wistar rats. The acute pancreatitis was induced by retrograde injection of 0.1 ml/0.1 kg body weight of 3% sodium taurocholate solution into the pancreatic duct as previously described by other authors. The experimental animals were divided into four groups: K0-control group (healthy animals), KP-animals with acute experimental pancreatitis without medication, S-rats with acute experimental pancreatitis, which were treated with 0.9% NaCl solution, and F-animals with acute experimental pancreatitis treated with Fibrolan. Fibrolan and 0.9% NaCl were injected into the peritoneal cavity three times a day with eight hours intervals from the 24th experimental hour. The serum amylase and lipase activities and the amylase activity in the urine were determined in each animal group in the 24th, 48th, 72nd, 96th, 120th, 144th experimental hour. For histopathological analysis pancreatic tissue samples were taken from the pancreatic gland. The intensification of the histological changes of these tissue samples was judged using a point score as described by Spormann et al. The results were statistically analysed. The animals of the KP group showed significant histological changes of the pancreas during the whole examination time. Point score: 75-100. Microscopically, tissue samples taken from the animals treated with Fibrolan showed less changes from the 72nd examination hour. There were observed regenerative processes and the improvement of the histological state was evident (point score: 0-50). Changes were less in rats treated with 0.9% NaCl solution than in KP group but the regenerative processes were slower than in F group (point score: 50-75). Enzymes levels were significant lower in F group than in others from the 48th experimental hour.

Amylases↗
PubMed1997

[Changes of lysosomal enzyme activity in the lungs during the course of acute pancreatitis].

The aim of this work is to study changes in activities of lysosomal enzymes of lungs in Acute Respiratory Distress Syndrome as complication in the course of experimental acute pancreatitis. The study was carried out on 100 male rats, Wistar strain 240-480 g weight. The healthy animals were used as control to find out to norms of activity of lysosomal enzymes of lungs (Cathepsin B, D, L, Acid phosphatase, Lipase, Sulfatase, B-D-galactosidase, N-Acetyl-B-glucosaminidase). The laparotomy was performed on 90 rats and sodium taurocholate (60 animals-OP group) and 0.9% NaCl (30 animals-OK group) was injected pancreaticoduodenal duct. The rats from OP and OK groups were divided into 5 subgroups. After 2, 6, 12, 24 and 48 hours after the operation, animals were sacrificed and the lungs were dissected, weighed, frozen. After thawing the lungs in 0.9% NaCl, 1 g of lungs was sampled at ice melting point. The obtained homogenate centrifuged, supernate was poured out and centrifuged again at a speed of 14,000 rot per min. The activity of enzymes was determined in using supernate preparations by Sigma. Quantitative determination were carried out using Lowrey's method. Differences in values of enzyme activity were statistically significant, between particular groups of animals OK and OP. The highest increase in the activity of enzymes was found between 12 to 24 hours after inducing the disease, what correlated with the increase in volume of lungs. The monitoring of activity of lysosomal enzymes is a sensitive indicator to evaluate the increase of ARDS in the course of experimental acute pancreatitis.

Acute Disease↗
PubMed1997

[Iatrogenic esophageal injuries and their treatment].

In our Department 22 patients were treated due to esophagus perforations in the years 1990-1996. Most of them (16) occurred after endoscopic examinations: diagnostic-3, after strictures dilatation-10, endoscopic intubation-2, sclerotherapy-1, transesophageal cardioversion-1. There were 5 intraoperative perforations: 3-after cardiomyotomy, 2-after intubation. The following treatment was performed: oesophageal suture and drainage-5 patients, subtotal oesophagectomy with oesophagogastrostomy-3 patients, oesophagectomy with salivary fistula and oesophago-gastro or -colostomy in second operation--9 patients, perforation covered with gastric fundus--3, intubation--1 patient, conservative treatment--1 patient. Four (18%) patient died.

Dilatation↗
PubMed1996

Relationships between divisions of the lingular bronchus and vascularization patterns in the lingula.

In 100 left human lungs the main bronchus, the pulmonary artery and the pulmonary veins were injected with 65% methyl methacrylate (Duracryl) and then digested in sulphuric acid. The resulting specimens were studied concerning the divisions of the lingular bronchus and the types of arterial and venous vascularization of the lingula. As a rule the lingular bronchus divided into two segmental bronchi. A single lingular artery was found in 80% of the cases and a single lingular vein in 58%. Atypical bronchial divisions were almost always associated with unusual types of vascularization. Patterns of bronchial division showed complete concordance with those of arterial vascularization of the lingula in 64% of the lungs and consistency with venous drainage patterns in 54%.

Adolescent↗
PubMed1996

Variability of modes of division of the superior lobar bronchi in the right and left lungs.

The studies were carried out on 100 right lungs and 100 left lungs taken from cadavers of humans of both sexes, aged 18 to 80 years. After swilling, the bronchi were injected with 65% duracryl and after 24 hours digested in sulphuric acid. The obtained casts were measured and the modes of division of superior lobar bronchi were determined. The results of observation and measurements were presented in the form of schematic drawings and documented with photographs. On the basis of the modes of branching of superior lobar bronchi three types and eight variants of division for the right lobar bronchus and two types and five variants for the left lobar bronchus were identified. The divergence between the frequency of some modes of division of the studied bronchi, especially according to authors from other countries and very similar results of investigations relating to atypical modes of division of some bronchi, performed in Poland and neighbouring countries, were noted.

Adolescent↗