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Biomedical subjects

R Maciejewski

Publications and source records attributed to R Maciejewski.

64 records · Page 4Linked to original sources
PubMed1989

[Topometry of the bronchial tree in Ceropithecus aethiops with special regard to presence of precordial lobes].

The trachea and bronchus were filled with duracryl solution and on the basis of casts got in this way the length and the diameter of individual lobes of the bronchial tree of 14 male and 16 female monkey have been examined. It was ascertained that almost all measurements were greater in the individuals of female sex. In both individuals among segmental basilar bronchus the greatest diameter had medial and anterior bronchus, both in the right and left lung.

Animals↗
PubMed1989

[The pulmonary artery size variability during various stages of post-fetal life in man].

Fresh lungs (45 right and 40 left) from dead bodies of 50 (30 male and 20 female) people from 1 to 80 years were examined. The pulmonary arteries were injected for 65% duracryl solution (Spofa-Dental firm) and after maceration the preparations were measured. Pulmonary arteries indices were also calculated. Pulmonary arteries diameter in male measured from 9.0 to 24 mm, average 17.0 mm, in female--from 7.4 to 28 mm, average 18.7 mm. The difference between the right and left pulmonary artery size was statistically significant, both in male and female persons. The correlation indices between size of male right pulmonary artery and age, length and body weight amounted to 0.70, 0.61 and 0.53, while for left pulmonary artery--0.58, 0.55 and 0.49. The same indices of female specimens valued for right pulmonary artery 0.72, 0.58 and 0.51. The size of the left pulmonary artery was correlated only with age (index 0.65).

Adolescent↗
PubMed1984

Antibody-dependent cell-mediated cytotoxicity of mononuclear cells against Langerhans islets of Wistar rats in normal man and in patients at diabetes risk.

Antibody-dependent cell-mediated cytotoxicity against pancreatic islets was investigated in 13 newly diagnosed insulin-dependent diabetics, in 38 patients at high risk for the disease and in 20 age-matched healthy controls. For this purpose 51Cr-labeled neonatal rat pancreatic islets incubated with the specific anti-rat islet cell antiserum 339 or with serum of the lymphocyte donors were used as targets. The antibody-mediated cytotoxic activity of mononuclear cells was evaluated from the specific chromium release after 6 h exposure of pretreated islets to the effector cells. The specific cytotoxic effect of mononuclear cells from healthy controls on pancreatic islets pretreated with serum is weak. ADCC mediated by the specific antirat islet cell antiserum is significantly increased in 54% of newly diagnosed diabetics as well as in 32% of patients at high risk for insulin-dependent diabetes mellitus. 46% of the newly diagnosed diabetics were also ADCC-positive when their own serum was used for the pretreatment of islets, regardless of whether they were islet cell antibody- or islet cell surface antibody positive or not. Subsets of mononuclear cells (non E-rosette-forming cells, high affinity E-rosette-forming cells, low affinity E-rosette-forming cells) were prepared and their cytotoxic potential was analysed in patients with positive ADCC test results.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMedDate not supplied

Activity of cathepsins in rat's spleen due to experimentally induced pancreatitis.

The aim of this study was to establish and quantify the changes of the level of cathepsin B, D and L in the spleen during experimental pancreatitis. The experiment was carried out in 115 male Wistar rats, randomly divided into three groups: intact (n = 15), injected with 0.9% NaCl solution into the common bile pancreatic duct (n = 50) and injected with 5% sodium taurocholate into this duct to induce acute pancreatitis (n = 50). After 2, 6, 12, 24 and 48 hours rats were anaesthetised, and blood was taken for amylase determination from the heart, and the spleen was removed. Alpha-amylase level in the blood serum samples was measured by enzymatic method. Cathepsin activity was established by spectrophotometric methods using substrates which form coloured complexes when they react with these proteases. The specific free fraction activity of cathepsin B, D and L in the spleen changed during the course of experiment, but there was no correlation between their activity and the intensity of pancreatitis established by serum amylase level.

Acute Disease↗
PubMedDate not supplied

Changes in the activity of lysosomal enzymes in rat kidneys in the course of acute pancreatitis.

BACKGROUND: The pathogenesis of complications of acute pancreatitis is an important subject of research for many experimenters. The aim of the research reported here was to evaluate changes in the activity of lysosomal enzymes in rat kidneys in the course of experimentally-induced acute pancreatitis. MATERIAL AND METHODS: 70 male Wistar rats of reproductive age with a mean body weight of 300 g were included in the study. Healthy animals were used as a control group to determine normal values for the activity of renal lysosomal enzymes: Cathepsin B, D, L, Acid Phosphatase, N-acetyl-beta-D-glucosaminidase, Lipase, Sulfatase, and Beta-D-Galactosidase. Acute pancreatitis was induced by injecting a 5% solution of sodium taurocholate into the hepatopancreatic duct. The animals were divided into 3 groups: I - healthy rats - normal group; II - rats with acute pancreatitis evoked by the injection of 5% sodium taurocholate; III - rats with a 0.9% solution of natrium chloride injected into the hepatopancreatic duct. Groups II and III were randomly divided into 6 subgroups. At 2, 6, 12, 24, 48, and 72 hours after the induction of acute pancreatitis the rats were anesthetized and their kidneys used for biochemical investigations. RESULTS: The differences in enzyme activity were statistically significant among the various subgroups of Groups II and III groups. The highest decrease in enzyme activity was found between 12 and 48 hours after the induction of the disease, followed by small increases up to 72 hours. CONCLUSIONS: The monitoring of lysosomal enzyme activity can be used to evaluate the increase of kidney failure in the course of experimentally-induced acute pancreatitis.

Acute Disease↗