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Biomedical subjects

R Manabe

Publications and source records attributed to R Manabe.

At least 109 records · Page 6Linked to original sources
PubMed1983

Fibronectin. A new therapy for corneal trophic ulcer.

Fibronectin, a glycoprotein, is present in plasma and extracellular matrix and is responsible for cellular adhesion. With the use of affinity chromatography, we purified plasma fibronectin from two patients with trophic corneal ulcer and persistent epithelial defect after conventional therapy was ineffective. Reepithelialization began three days after initiation of treatment with autologous purified fibronectin eyedrops, and the epithelial defects disappeared completely within three weeks. No recurrence was observed after treatment ended. By modifying the original chromatographic procedure, we were able to obtain purified fibronectin in two hours, which allowed us to treat outpatients as well as inpatients.

Adult↗
PubMed1983

Incidence of cystoid macular edema after retinal detachment surgery and the use of topical indomethacin.

We conducted a prospective, double-masked, controlled study to determine the effects of indomethacin in preventing the development of cystoid macular edema after retinal detachment surgery. In addition to routine medication, topical indomethacin was applied to 63 eyes (52 with intact lenses and 11 aphakic) and a placebo was applied to 61 eyes (51 with intact lenses and ten aphakic) that underwent retinal detachment surgery. Indomethacin drops or placebo were instilled once an hour for five hours preoperatively and three times daily for four weeks postoperatively. Fluorescein angiography, done before surgery and four to six weeks after surgery to detect cystoid macular edema, showed that cystoid macular edema developed in 11 of 63 eyes (13%) in the indomethacin-treated group and in 20 of 61 eyes (33%) in the placebo group (P less than .01). Five of 28 eyes (18%) with cystoid macular edema in both groups and 38 of 96 eyes (40%) without cystoid macular edema in both groups had visual acuities of 20/40 or better (P less than .05).

Administration, Topical↗
PubMed1983

Fibronectin promotes epithelial migration of cultured rabbit cornea in situ.

We investigated the effect of fibronectin on epithelial migration onto the stroma in cultured rabbit cornea. Rabbit plasma fibronectin was purified by affinity chromatography using gelatin-Sepharose 4B, and its purity was confirmed by SDS polyacrylamide slab gel electrophoresis. Antibody against rabbit plasma fibronectin raised in guinea pigs formed a single precipitin line against rabbit plasma and purified rabbit plasma fibronectin by Ouchterlony double diffusion test. When rabbit cornea was cut into small blocks and cultured in TCM-199 medium alone, corneal epithelial cells began to migrate on the cut edge of the corneal stroma. The addition of purified rabbit plasma fibronectin to the culture medium significantly enhanced epithelial migration. The degree of enhancement depended on the amount of fibronectin added. When guinea pig IgG anti-rabbit plasma fibronectin was added, epithelial migration was significantly inhibited when compared with that in control cultured corneal blocks. The results demonstrate that fibronectin promotes epithelial migration in the cornea and thus plays an important role in corneal wound healing.

Animals↗
PubMed1983

Appearance of fibronectin in rabbit cornea after thermal burn.

The sequence of the appearance of fibronectin in the rabbit cornea after thermal burn was investigated by indirect immunofluorescent microscopy. One day after thermal burn, the keratocytes disappeared completely from the burned area, and faint fibronectin-specific fluorescence was detected beneath the epithelium and on the stromal side of the Descemet's membrane. On day 3, activated keratocytes began to migrate to the burned lesion. Fibronectin was detected in the burned area as well as in the surrounding uninjured area, and on both sides of the Descemet's membrane. One week after the burn, the keratocytes had increased in the lesion and, concomitantly, fibronectin-specific fluorescence could be observed at the locations corresponding to the keratocytes. Fibronectin in the surrounding area gradually diminished. After 4 weeks, activated keratocytes had accumulated in the lesion, and fibronectin was detected only in the central lesion and at the level of the Descemet's membrane. Thus the appearance of fibronectin was concordant with the appearance of the keratocytes, and this suggests that fibronectin may be involved in the wound-healing of thermally burned rabbit corneas.

Animals↗
PubMed1983

Long-term observations of the graft endothelium with different postoperative courses.

Using a specular microscope and a pachymeter, we monitored endothelial cell patterns and central corneal thickness in 16 cases of clear graft with different postoperative courses for over 2 years after successful penetrating keratoplasty. With the use of a computerized digitizer, the graft endothelium was analyzed to determine the mean cell size, standard deviation of cell sizes and the coefficient of variation in cell size. In all cases, a progressive increase in the mean and standard deviation of cell sizes was noted throughout the period of observation. The coefficient of variation, however, stayed fairly constant in each case. This indicates that the increase in the standard deviation of cell sizes is dependent on the increase in the mean cell size. Five cases with good postoperative course showed gradual alterations in cellular morphology. On the other hand, the endothelium of 5 cases with surgical intervention after keratoplasty changed its morphology greatly until the corneal thickness approximated normal, and then the alterations continued to take place gradually. In 6 cases, a remarkable and rapid increase in the mean and standard deviation of cell sizes occurred during the rejection process. These parameters continued to increase gradually after the graft became transparent as the result of intensive corticosteroid therapy. These results suggest that the graft endothelium is still in a transitional state during healing even 2 years after keratoplasty, and that the morphologic parameters determined during the early postoperative period reflect the actual cell damage owing to surgical procedures to some extent but not exactly.

Adolescent↗
PubMed1982

Fibronectin in corneal wound healing: appearance in cultured rabbit cornea.

Fibronectin (FN) has been detected at the site of corneal wounds. To investigate whether FN is supplied by the cornea itself or by extracorneal sources, we cultured small blocks of rabbit cornea in vitro with and without autologous serum (5%). The sliding rate of epithelial cells was determined and localization of FN in the corneal block examined by indirect immunofluorescent microscopy. Epithelial cells slid more rapidly in the presence of serum and covered most of the stromal surface in about 24 hours. FN was detected at the surface edge of the cut stroma shortly after culturing began. During epithelial cell sliding, FN-specific fluorescence was noted beneath the migrating cells. The location of FN was essentially the same in cultures with and without autologous serum. These results suggest that FN might be supplied by the cornea itself and that it may play a role in epithelial cell sliding.

Animals↗
PubMed1982

Rapid preparation of purified autologous fibronectin eyedrops from patient's plasma.

Fibronectin (FN), a glycoprotein present in plasma and the extracellular matrix, has been reported to be effective on corneal trophic ulcer. This study reported a rapid method for preparing purified FN eyedrops from a patient's own plasma. Plasma FN concentration in healthy Japanese was found to be 235 +/- 62 micrograms per ml(age 21-31 years old). Men had a higher plasma FN level than women (264 +/- 62 vs. 206 +/- 44 micrograms/ml). The FN eyedrops were prepared by gelatin-coupled Sepharose 4B affinity chromatography and gel filtration by Sephadex G-25 (PD-10). Biochemical and immunochemical analyses revealed that the prepared FN eyedrops contained electrophoretically pure FN and no other plasma proteins were detected. Average FN concentration in the eyedrops was 425 micrograms per ml and recovery rate was 54%. The FN eyedrops were found to be stable at room temperature up to 10 days in biological activity as far as we examined. The total time required to prepare the FN eyedrops was within 2 hours. Thus it is possible to apply them to outpatients as well as inpatients.

Adult↗
PubMed1979

Analysis of herpes simplex virus isolated from patients with recurrent herpes keratitis exhibiting "treatment-resistance" to 5-iodo-2'-deoxyuridine.

Four strains of herpes simplex virus (HSV) were isolated from two patients with recurrent herpes keratitis who failed to respond to 5'-iodo-2'-deoxyuridine (IDU) treatment. Two of the four isolates were highly resistant to IDU in cell culture and the other two isolates were more susceptible to IDU than an HSV-1 laboratory strain. From each patient, an IDU-resistant and an IDU-susceptible virus was isolated. All 4 isolates possessed the ability to induce the thymidine kinase (TK) activity in cell lines lacking that activity. All the isolates were type 1 HSV, since the filamentous structures, recognized as a biological marker of type 2 HSV, were not observed in infected cells.

Animals↗