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R Manabe

Publications and source records attributed to R Manabe.

122 records · Page 7Linked to original sources
PubMedDate not supplied

Fibronectin appears at the site of corneal stromal wound in rabbits.

Fibronectin (FN) is present both in plasma and in extracellular matrix, and is postulated to be involved in wound healing. The appearance of FN was investigated in the wounded rabbit cornea after corneal stroma was injured with a sharp knife or after lamellar keratoplasty was performed. An indirect immunofluorescence technique was employed to detect the presence of FN. Normal, unwounded rabbit cornea showed an intensive fluorescence at Descemet's membrane. Strong specific fluorescence was observed at the edge of a stromal wound and beneath the sliding epithelial cells after non-perforating incisions. These could be detected as early as 3 hours after injury. The fluorescence became the strongest at 6 hours, and and then gradually decreased in its intensity, disappearing by about 7 days when epithelial cells entirely filled the stromal wound. In case of lamellar keratoplasty, FN was detected at the interface of the graft and the recipient cornea. These results suggest that FN plays an important role in the stromal wound healing.

Animals↗
PubMedDate not supplied

Sodium and chloride transport across the isolated rabbit ciliary body.

Unidirectional Na+ and Cl- fluxes were determined in the isolated rabbit ciliary body under short-circuited conditions. A statistically significant net Cl- flux towards aqueous was detected in the standard bathing solution (HCO3- = 115 mM), but no net Na+ flux was demonstrated in this solution. The net Cl- flux exceeded the short-circuit current, suggesting the existence of a net flux of other ion(s). In a bathing solution containing 5 mM HCO3-, the net Cl- flux was abolished, suggesting that HCO3- in the bathing solution enhances the net Cl- flux.

Action Potentials↗