A quantitative evaluation of three blood culture systems.
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Biomedical subjects
Publications and source records attributed to R Rosner.
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By utilizing parallel culture methods on multiple blood specimens, three blood culture systems were evaluated for their ability to allow rapid recovery of organisms. The same base medium was used for all three systems. System or flask 1 contained base medium with Liquoid added, flask 2 contained base medium with sucrose added, and flask 3 contained both Liquoid and sucrose. During the study period, there were 259 patients considered to have positive blood cultures. Organism recovery was accomplished from flask 1 in 214 of these patients, from flask 2 in 180 cases, and from flask 3 in 257 instances. The use of anticoagulants and osmotic stabilizers in blood culture systems is discussed in detail.
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Rosner, Richard (St. Joseph's Hospital, Paterson, N.J.). Isolation of Candida protoplasts from a case of Candida endocarditis. J. Bacteriol. 91:1320-1326. 1966.-A case of endocarditis caused by Candida tropicalis is described. Even though the patient was receiving adequate therapy, and all routine blood cultures were negative for growth, the patient continued to give clinical evidence of active, progressive endocarditis. The isolation of osmotically fragile bodies from blood cultures placed in an osmotically controlled medium is described in detail. The role of these bodies, called protoplasts, in the active disease process of this patient is discussed in relation to the criteria for the implication of protoplasts in the disease process. Several explanations as to what caused the in vivo formation of protoplasts of C. tropicalis in this patient are discussed.
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