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Biomedical subjects

R S Pandey

Publications and source records attributed to R S Pandey.

At least 37 records · Page 2Linked to original sources
PubMed1989

Toxicity of endosulfan on kidney of male rats in relation to drug metabolizing enzymes and microsomal lipid peroxidation.

Endosulfan administration (po, 15 and 30 days at 7.5 and 10 mg/kg body wt respectively) inhibited the activity of microsomal mixed function oxidases in kidney tissue of male rats. Microsomal and cytosolic protein contents of kidney were significantly increased following 30 days endosulfan exposures. Profound induction in the activity profiles of alcohol dehydrogenase and cytosolic glutathione s-transferase was noticed, however, no such change was apparent in the activity of aldehyde dehydrogenase. Microsomal preparations from treated animals showed a dose and duration dependent increase in spontaneous lipid peroxidation. The observed biochemical changes persisted even after 7 days normalcy allowance provided after the endosulfan (10 mg/kg body wt) withdrawl. The results suggest a substantial renal toxicity of endosulfan to male rats in relation to microsomal mixed function oxidases and associated functions which possibly resulted from lipid peroxidative damage of microsomal membrane in treated animals.

Animals↗
PubMed1984

Analysis of chromatin of skeletal muscle of developing rats using micrococcal nuclease and DNase I.

Conformational changes in the chromatin of skeletal muscle of 3-, 14- and 30 day-old developing rats have been studied using DNase I and micrococcal nuclease (MCN). Purified nuclei were digested separately by MCN and DNase I. The rate and extent of digestion by MCN decreases gradually as development proceeds. The electrophoretic pattern of MCN digested DNA, however, shows no change. The kinetics of digestion of nuclei by DNase I show no change with development. However, the electrophoretic pattern of DNase I digested DNA shows a gradual decrease in the amount of 10-30 bp fragments with progressive development. These studies show that the chromatin of the skeletal muscle undergoes certain conformational changes during postnatal development, and such changes in chromatin may be necessary for terminal differentiation of this tissue.

Age Factors↗
PubMed1984

Developmental changes in chromatin of skeletal muscle of rats: acetylation of chromosomal proteins and transcription.

In vivo acetylation of chromosomal proteins and RNA synthesis were studied in the skeletal muscle of 3-30 day old developing rats. The levels of acetylated histones and nonhistone chromosomal (NHC) proteins are high at day 3 and decrease as development progresses. Spermine has no significant effect on acetylation. Incorporation of 3H-Uridine into RNA of both nuclear and cytoplasmic fractions is also maximum at day 3 and plateaus by day 14. Nuclear RNA synthesis following acetylation of chromosomal proteins is greatly stimulated in all the ages studied, whereas that in cytoplasmic RNA occurs only in 3 day old rats. Such modifications during early development may bring about conformational and functional changes in the chromatin and contribute significantly to the process of terminal differentiation of skeletal muscle cells.

Acetylation↗
PubMed1984

Prolactin in blood plasma and milk of buffalo during estrous cycle and early pregnancy.

Concentration of prolactin was measured by radioimmunoassay for 11 primiparous and 16 multiparous buffalo. Prolactin concentration of blood plasma on day of insemination was about 200 ng/ml for primiparous and about 315 ng/ml for multiparous animals and fluctuated between 130 and 200 ng/ml for primiparous and between 250 and 345 ng/ml for multiparous pregnant animals. For the nonpregnant group prolactin fluctuated between 145 and 240 ng/ml for primiparous and between 210 and 310 ng/ml for multiparous animals with minor elevations 1 to 2 days before estrus. Concentration of prolactin of milk was not significantly different from that of plasma and was positively correlated .68 for nonpregnant and .93 for pregnant animals.

Animals↗
PubMed1983

Luteinizing hormone in blood plasma of post-partum buffaloes (Bubalus bubalis).

The changes in luteinizing hormone concentration were measured by heterologous double-antibody radioimmunoassay in blood plasma of 28 Murrah buffaloes. The LH concentration fluctuated between 0.22 to 0.78 ng/ml during first 21 days post-partum. The level increased significantly (P < 0.001) at estrus. The basal LH concentration of second and third week post-partum was inversely related to the first post-partum ovulation interval.

Journal Article↗
PubMed1983

Gonadal steroid hormone level in blood plasma and milk of primiparous and multiparous nonpregnant and pregnant buffaloes.

Changes in the concentration of progesterone and estradiol-17beta were measured by radioimmunoassay in 11 primiparous and 17 multiparous buffaloes at estrus and daily post insemination and in 6 nonbred buffaloes at 6 hour intervals from 4 days before expected estrus to one day after estrus. Plasma progesterone concentration at estrus was 0.1 ng/ml which rose to a peak level of 3.47 ng/ml on day 17. It fluctuated around this level in those animals which conceived, but followed a declining trend in those which failed to do so and attained lowest values on the day of next estrus. Temporal changes of the hormone revealed that the occurrence of major decline varied between 16 and 62 h before estrus. The average concentration in milk was about three to four times higher than in plasma. The concentration of estradiol-17beta about 23.50 pg/ml at estrus and fluctuated around 10 pg/ml in animals that returned to estrus with a peak around estrus. Temporal changes of hormone revealed that peak level occurred 8-17 h before estrus. The concentration of estradiol in pregnant animals fluctuated around 10 pg/ml. The concentration in milk was about 2-3 times higher than in plasma. There was no significant (P>0.05) difference in the concentrations of progesterone and estradiol-17beta between primiparous and multiparous animals.

Journal Article↗
PubMed1983

Prostaglandin F2 alpha in blood plasma and milk of non-pregnant and pregnant buffaloes (Bubalus bubalis).

Radioimmunoassay of prostaglandin F2 alpha (PGF2 alpha) was standardised for sufficient precision, accuracy, sensitivity and specificity. The changes of PGF2 alpha were monitored in peripheral blood plasma and milk of 28 Murrah buffaloes. There were considerable individual variations of PGF2 alpha concentration in blood plasma (27.6 to 110.8 pg/ml) and milk (80.3 to 180.4 pg/ml). A non-significant increase of PGF2 alpha concentration (P greater than 0.05) was observed at the end of the cycle of non-pregnant animals in both fluids but no such increase was observed in pregnant animals. The concentration of PGF2 alpha in milk was significantly higher (P less than 0.01) than that of blood plasma and was positively correlated in non-pregnant (r = 0.738) and pregnant animals (r = 0.385).

Animals↗
PubMed1983

Seasonal variations in oestradiol-17 beta and luteinizing hormone in the blood of buffalo cows (Bubalus bubalis).

Changes in the plasma concentrations of oestradiol-17 beta and LH were measured in six buffaloes exhibiting regular oestrous cycles for one calendar year. Significantly higher concentrations of oestradiol-17 beta and LH were observed around oestrus during the cooler months than during the hotter months whereas no significant fluctuations were observed in the basal levels of either hormone throughout the year.

Animals↗