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R Shibata

Publications and source records attributed to R Shibata.

At least 127 records · Page 7Linked to original sources
PubMed1986

Feeding and diurnal related activity of lateral hypothalamic neurons in freely behaving rats.

Activity of 64 single neurons in the lateral hypothalamus (LHA) was recorded for 1-8 days in freely behaving rats. The activity of 26 (40.6%) neurons varied with circadian rhythm and in relation to feeding. Activity of 23 of these neurons decreased during consumption of each pellet, and that of one increased. The activity of two other neurons increased intermittently, at night, prior to and during eating and drinking episodes. All changed activity with sleep-wake changes; increasing in the dark or upon arousal, and decreasing in the light or during slow wave sleep, but the activity was independent of individual movement, except feeding. The activity of 29 (45.3%) neurons varied only diurnally. Of these, 26 neurons also had sleep-wake responses and activity changes that corresponded to behavior. The firing rate of the other 3 neurons was independent of sleep-wake condition or individual feeding activity, but gradually increased in the dark to a maximum in the early morning, then subsided rapidly in 1-2 h. Four (6.3%) neurons were related only to feeding and not to diurnal rhythm, and 5 (7.8%) neurons were not related to either. Of the 5 neurons that were unrelated to either diurnal rhythm or feeding acts, 3 increased activity at light on and decreased it at light off for 4-13 min. These data suggest LHA neuronal involvement in control of short term feeding or individual feeding episodes, and long term feeding or circadian feeding rhythm.

Animals↗
PubMed1986

A microcomputer-based system for detailed analysis of rat behavior.

A microcomputer-based system that can measure rotational behavior and moving velocity is described. Infrared photobeams detect the animal behavior. One advantage of this system is that data can be stored on floppy disks and analyzed in arbitrary time intervals. This system may be useful for detailed analysis of rat behavior in physiological or psychopharmacological experiments.

Animals↗
PubMed1985

Placental-soluble aconitase polymorphism in Japanese.

Polymorphism of soluble aconitase was investigated in 152 Japanese placentae. The allelic frequencies were ACONS1 = 0.951 and ACONS2 = 0.049. ACONS2 appears to be rather high among Orientals including Japanese, while ACONS4 seems to be characteristic for Negroids.

Aconitate Hydratase↗
PubMed1985

A further study on the polymorphism of placental alkaline phosphatase in Japanese.

Polymorphism of placental alkaline phosphatase (PL) was investigated in 120 Japanese placentae by means of starch gel electrophoresis and an improved staining method for the isoenzymes using naphthol AS-MX phosphate as substrate instead of beta-naphthyl phosphate. The present method gave more satisfactory results for the detection of the PL bands. Besides five of the six common phenotypes three different uncommon variants were identified. The gene frequencies of PL types were estimated at PL1 = 0.729, PL2 = 0.054, PL3 = 0.200 and PLv = 0.017, which were very similar to those reported in other districts of Japan.

Alkaline Phosphatase↗
PubMed1985

Polymorphism of placental phosphoglucomutase in Japanese.

Polymorphism of PGM1 and PGM3 types was investigated in placental extracts from 127 unrelated Japanese parturients living in Yamanashi Prefecture. The gene frequencies were 0.787 for PGM1(1), 0.213 for PGM1(2); 0.760 for PGM13, 0.240 for PGM3(2), respectively. There was no significant association between the PGM1 and PGM3 systems. The PGM3(2) frequency in the present sample population was higher than those in Kanto District, but lower than those in Kinki District.

Electrophoresis, Starch Gel↗
PubMed1984

Selective IgA deficiency: analysis of Ig production in vitro.

The cellular basis of the pathogenesis of selective IgA deficiency (SIgAD) was investigated by examining surface immunoglobulin (SmIg) and in vitro pokeweed mitogen (PWM)-stimulated immunoglobulin (Ig) synthesis and by assaying in combination the counterpart lymphocytes from individuals with SIgAD and healthy donors. Peripheral blood lymphocytes (PBL) from 14 individuals with SIgAD synthesized normal amounts of IgG and IgM but did not synthesize normal amounts of IgA. Functional defects of lymphocytes for IgA synthesis were classified into four types: (i) B-lymphocyte dysfunction, (ii) increased function of suppressor T lymphocytes (Ts), (iii) decreased function of helper T lymphocytes (Th), and (iv) B-lymphocyte dysfunction and increased Ts function. The cells bearing SmIgG, SmIgM, and SmIgD were demonstrated at normal percentage ratios in all cases by immunofluorescent staining. The cells bearing SmIgA were at normal percentage ratios in the cases of T-lymphocyte dysfunction, while in the cases of B-lymphocyte defect SmIgA-bearing cells were reduced.

Adult↗
PubMed1984

A stability study on Gc subtyping in bloodstains: comparison by two different techniques.

The limits of determination of Gc subtypes in bloodstains were compared between the immunofixation method and the sulfosalicylic acid precipitation method using isoelectric focusing on polyacrylamide gel. By the immunofixation method Gc subtyping in bloodstains was successfully made at 37 degrees C after 7 weeks, at room temperature after 17 weeks and at 4 degrees C even after 25 weeks storage. By the sulfosalicylic method Gc subtypes were no longer able to be determined a few weeks after stain formation. The superiority of the results obtained by the immunofixation method makes it the recommended method for the Gc subtyping from bloodstains in medicolegal practice.

Benzenesulfonates↗
PubMed1984

Interaction of morphine-epoxide with multiple opiate receptors.

We examined the interaction of morphine-epoxide, which was assumed to be a new metabolite of morphine, with opiate receptor subtypes using pharmacological and biochemical techniques. Morphine-epoxide was about 3 to 4 times less potent than morphine to the interactions of opiate receptors. However, the ratio of IC50 values for the guinea pig ileum and mouse vas deferens to electrical field stimulation and the ratio of IC50 values for the [3H]-dihydromorphine and [3H]-D-ala2-D-leu5 enkephalin binding to rat brain membrane preparations of morphine-epoxide was similar to those of morphine. Morphine-epoxide had virtually no effect on the twitch responses of rabbit and rat vas deferens. Furthermore, "sodium ratio" and "GTP ratio" of morphine-epoxide were similar to those of morphine and differed from naloxone. These results suggest that morphine-epoxide as well as morphine behaves as the agonist on the mu type opiate receptor notwithstanding that the affinity of morphine-epoxide is slightly less than that of morphine.

Analgesics↗
PubMed1982

Photoaffinity labeling of opioid receptor with morphine-7,8-oxide (morphine epoxide).

The opioid receptor mediating inhibitory action of morphine in the electrically stimulated guinea pig ileum was irreversibly photoinactivated by morphine epoxide (3 X 10(-6) M). Morphine epoxide (up to 3 X 10(-5) M) did not influence the responses of rat vas deferens (epsilon-receptor) or rabbit vas deferens (kappa-receptor) to electrical stimulation. Effective concentrations of morphine epoxide were much lower in the guinea pig ileum (mu-receptor) than in the mouse vas deference (delta-receptor). The inhibitory action of [Met]-enkephalin on the twitch responses of the rat vas deferens and mouse vas deferens to electrical stimulation were not influenced after irradiation in the presence of morphine epoxide (3 X 10(-6) M). Therefore, morphine epoxide is probably a useful probe for photoaffinity labeling of the mu-receptor in vitro.

Affinity Labels↗
PubMed1982

Some pharmacological properties of morphine-7,8-oxide (morphine epoxide).

Morphine-7,8-oxide (morphine epoxide) is assumed to be a metabolite of morphine. Morphine epoxide in antinociceptive action was practically as potent as morphine. The development of tolerance in antinociceptive action was slower in the rats treated with morphine epoxide than in the rats with morphine. Furthermore, morphine epoxide was less potent than morphine in the inhibition of abstinence syndrome.

Analgesics↗
PubMed1979

Simple and rapid method for the diagnosis of chronic granulomatous disease, measuring hydrogen peroxide and superoxide anions released from leukocytes in whole blood.

A simple quantitative method to measure release of hydrogen peroxide and superoxide anions from leukocytes using less than 10 microliter blood is described. Whole blood instead of a leukocyte suspension was used. The release of hydrogen peroxide was measured fluorometrically by adding a blood sample to an assay mixture containing homovanillic acid, peroxidase and azide. The release of superoxide anions was measured spectrophotometrically with a dual wavelength spectrophotometer by adding a blood sample to an assay mixture containing cytochrome C. The normal values of the releasing activities are given.

Concanavalin A↗