PubMed Health⌕ Search

Biomedical subjects

R Stauber

Publications and source records attributed to R Stauber.

At least 37 records · Page 2Linked to original sources
PubMed1995

Quantitative perfusion parameters of focal nodular hyperplasia and normal liver parenchyma as determined by electron beam tomography.

Eight patients with known focal nodular hyperplasia (FNH) were studied by electron beam tomography (EBT) in a multisection flow mode at defined time intervals after intravenous contrast agent injection. Various regions of interest (ROIs) were defined in the areas of FNH, normal liver parenchyma, aorta and portal vein. In each ROI density changes were measured and plotted against time. In those time-density curves (TDCs) the slope, the time of maximal enhancement and the absolute density value of peak enhancement were determined. Arterial tissue perfusion was calculated for normal liver parenchyma and FNH. In all eight patients significant differences in peak density (123.88 +/- 18.92 HU vs 102.5 +/- 15.49 HU, p = 0.012), in the rate of arterial contrast enhancement (252.63 +/- 28.05 HU min-1 vs 38.88 +/- 4.19 HU min-1, p = 0.012) and in the arterial tissue perfusion (1.36 +/- 0.19 ml min-1 cm-3 vs 0.21 +/- 0.03 ml min-1 cm-3, p = 0.012) between FNH and normal liver parenchyma were found. Perfusion values of normal liver parenchyma correspond to those determined by inert gas clearance. Further studies will determine the practicability of this quantitative method in the evaluation of global and other focal liver diseases.

Adolescent↗
PubMed1994

Transjugular intrahepatic portosystemic shunt: angiographic follow-up and secondary interventions.

PURPOSE: To evaluate transjugular intrahepatic portosystemic shunt (TIPS) placements and secondary interventions. MATERIALS AND METHODS: Thirty shunt venograms were obtained in 21 patients with TIPS placement at 6-month intervals or if Doppler ultrasound showed an abnormality. RESULTS: Abnormalities in the shunt were shown in 24 venograms (80%). Eight occlusions (two early, five late, one repeat) and 16 stenoses (nine hepatic vein, six hepatic vein and stent, one stent only) were seen. Two patients with shunt abnormalities experienced recurrent bleeding. All others had no signs of recurrent portal hypertension. Occluded TIPS were recanalized (n = 5) or a new TIPS was placed parallel to the occluded one (n = 2). One repeat occlusion was not revised. Fifteen of 16 stenoses were treated successfully with balloon catheter dilation (n = 8) or additional stent placement (n = 7). The mean portal pressure gradient before revision was 21 mm Hg +/- 3.8 (standard deviation) and 13 mm Hg +/- 3.6 after revision. CONCLUSION: Stenosis and occlusion of TIPS may be common during long-term follow-up. Regular examinations may demonstrate abnormalities early. An abnormality seen on the shunt venogram usually can be revised during the same procedure.

Adult↗
PubMed1993

Proteolytic cleavage of the murine coronavirus surface glycoprotein is not required for its fusion activity.

The surface glycoprotein (S) of the murine hepatitis coronavirus MHV normally undergoes proteolytic cleavage during transport to the cell surface. To determine whether the cleavage of the MHV-JHM S glycoprotein is required to activate its ability to fuse cellular membranes, the protease recognition sequence in a cDNA copy of the S gene was altered from Arg-Arg-Ala-Arg-Arg into Ser-Val-Ser-Gly-Gly by site directed mutagenesis. The mutated and wild type S genes were expressed by means of recombinant vaccinia viruses and it could be shown that the mutated S protein was not cleaved when it was expressed in mouse DBT cells, in contrast to the wild type S protein. Nevertheless, the non-cleaved S protein induced extensive syncytium formation in mouse DBT cells. These results clearly indicate that the non-cleaved form of the MHV S protein is able to mediate cell membrane fusion. Thus, proteolytic cleavage is not an absolute requirement for its fusion function.

Amino Acid Sequence↗
PubMed1993

[Clinical experiences with the transjugular intrahepatic portocaval shunt (TIPS)].

Twenty-five patients with liver cirrhosis and portal hypertension were admitted for creation of a transjugular intrahepatic portosystemic shunt (TIPS). The procedure was successful in 22 patients (technical success 88%). The mean portal pressure gradient was lowered from 24.5 mmHg before to 11.4 mmHg after TIPS. Two early and three late occlusions were observed (primary patency rate 78%). The rate of secondary interventions was 41%. Five times a hepatic vein stenosis was dilated and stented, two times an occluded shunt was recanalized, two times a new shunt was created parallel to an occluded (secondary patency rate within a maximum of 16 months 95%). In two patients sepsis occurred which was effectively treated with antibiotics, two patients died shortly after TIPS due to hepatorenal syndrome and hepatic failure, respectively. There was no recurrent bleeding. Two patients developed hepatic encephalopathy; both improved after protein restriction. The authors conclude that TIPS is an alternative procedure to shunt surgery, especially for patients who cannot benefit from sclerotherapy.

Adult↗
PubMed1993

Proteolytic cleavage of the murine coronavirus surface glycoprotein is not required for fusion activity.

A cDNA copy of the murine coronavirus [otherwise known as murine hepatitis virus (MHV)] surface (S) glycoprotein gene was isolated and expressed in DBT cells by using a recombinant vaccinia virus system. The expressed S protein induced extensive syncytium formation at neutral pH. Oligonucleotide mutagenesis was used to engineer an S protein gene in which codons for the proteolytic cleavage site, Arg-Arg-Ala-Arg-Arg, were replaced with an equal number of codons for amino acids with aliphatic or aliphatic hydroxyl side-chains. The mutated S protein was stably expressed in DBT cells and, in contrast to the wild-type protein, was not proteolytically cleaved. Nevertheless, the non-cleaved protein induced extensive syncytium formation. These results clearly indicate that the non-cleaved form of the MHV S protein is able to mediate cell membrane fusion. Thus proteolytic cleavage is not an absolute requirement for fusion activity.

Amino Acid Sequence↗
PubMed1992

Air trapping from torsion of a congenital cyst of the lung.

Report on a case of congenital cyst of the lung which was attached to the pleura of the right upper lobe and because of torsion of its pedicle produced air trapping and mediastinal shift. Etiology, diagnosis and surgical indications are reviewed from the literature. No similar case has been previously reported.

Child, Preschool↗
PubMed1991

Analysis of murine coronavirus surface glycoprotein functions by using monoclonal antibodies.

The murine coronavirus surface glycoprotein gene was expressed as a fusion protein in bacteria, and the expressed protein was used to generate S protein-specific monoclonal antibodies (MAbs). Three of the MAbs, 11F, 30B, and 10G, were able to neutralize virus infectivity, and two of them, 11F and 10G, were able to block virus-induced, cell-to-cell fusion. The binding sites of the 11F, 30B, and 10G MAbs were determined by Western immunoblotting and epitope mapping. The 11F and 30B MAbs bound to sites located, respectively, between amino acids 33 to 40 and 395 to 406 in the amino-terminal (S1) subunit of the S protein, and the 10G MAb bound to a site located between amino acids 1123 and 1137 in the carboxy-terminal (S2) subunit. These data define more precisely the interactions between the S1 and S2 subunits of the murine coronavirus S protein and provide further insights into its structure and function.

Animals↗
PubMed1990

[Clinical aspects and therapy of gallstone ileus].

High lethality of gallstone ileus can be reduced by prompt diagnosis and careful treatment. Aerobilia is of great diagnostic value, in doubtful cases Gastrografin passage of the intestine is useful. Enterolithotomia alone is the least dangerous treatment. However, postoperatively the possibility of a recidive should be kept in mind.

Aged↗
PubMed1990

[Cowden syndrome].

A patient with multiple hamartoma syndrome or Cowden's disease with multiple gastrointestinal polyps and malignant melanoma is presented. The syndrome is characterized by hamartomatous tumors of the skin, fibrocystic disease of the breasts, gastrointestinal polyps and disease of the thyroid gland such as goiter and adenoma. In addition, other abnormalities and malformations occur in the skeletal system, central nervous system and urogenital tract. Recognition of this syndrome is important because of the association with malignant tumors of the breast and thyroid gland and with malignant melanoma. Gastrointestinal hamartomatous polyps may lead to the diagnosis of Cowden's disease which must be separated from other intestinal polyposis syndromes.

Adenomatous Polyposis Coli↗
PubMed1987

[Pathologic enzyme patterns in sonographically verified liver metastases].

The serum enzyme pattern, consisting of GOT, GPT, lactate dehydrogenase, gamma glutamyl transpeptidase and alkaline phosphatase, was investigated in 128 patients with sonographically verified liver metastases. Gamma glutamyl transpeptidase and alkaline phosphatase turned out to be the most sensitive enzymes, being elevated in 89% and 88%, respectively. The GOT was elevated in 45% GPT in 37.5% and lactate dehydrogenase in 56% of all cases. The enzyme elevation did not correlate with the degree of liver involvement. In conclusion, pathological serum enzyme patterns are useful for the detection and follow up of liver metastases. Normal serum enzyme levels do not rule out the presence of liver metastases.

Enzymes↗
PubMed1987

[Retained gallstones with reference to their nonsurgical treatment].

In a series of 1122 cholecystectomies we observed 15 cases of retained bile duct stones. Early postoperative diagnosis was achieved by T-drain-cholangiography. The application of T-drains was afflicted with a morbidity rate of 3.5%. For treatment we performed 2 relaparotomies, 3 percutaneous extractions, 5 endoscopic extractions, 1 lysis and 1 extracorporal shock-wave-lithotripsy. Using nonsurgical methods there are only few indications for open explorative choledochotomy.

Adult↗
PubMed1986

[Sonographic comparative studies of the portal vascular system before and following endoscopic sclerosing of esophageal varices].

On the occasion of 35 episodes of oesophageal injection therapy in which partly the intravascular and partly the perivascular injection technique were used, real time sonographic measurements were performed on the veins of the portal system immediately before and after sclerotherapy. In the case of intravascular injection, both the portal and the splenic vein and the confluens of the splenic and the superior mesenteric vein showed an increase in diameter, whereas in the cases of perivascular injection such changes were minimal or absent. The practical applicability of such sonographic calibrations could possibly lie in certain conclusions that could be drawn concerning the degree of vascular compression or obliteration during each session of sclerotherapy.

Esophageal and Gastric Varices↗