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R Tomecki

Publications and source records attributed to R Tomecki.

At least 37 records · Page 2Linked to original sources
PubMed1980

Cytotoxic effect in vitro of B lymphocytes and killer (K) cells in ulcerative colitis.

A mixture of B lymphocytes and K cells was isolated from serum of 18 patients suffering from ulcerative colitis (U.C.) and of 10 healthy individuals constituting the control group. The cytotoxic effect of these cells in vitro on the large bowel epithelial cells was examined. It was found that the mixture of B lymphocytes and K cells obtained from the patients suffering from ulcerative colitis destroyed the epithelial cells of large bowel, and that the cytotoxic effect depended primarily upon a degree of the severity of the disease. The results obtained confirmed our suggestion that B lymphocytes and K cells cooperate directly in the cytotoxic reaction.

Antibody-Dependent Cell Cytotoxicity↗
PubMed1978

In vitro cytotoxic effect of lymphocytes and their extracts in inflammatory bowel diseases. I.

From the sera of 20 patients with ulcerative colitis (U.C.) 10 patients with Crohn's Disease (C.D.) and 10 volunteers, lymphocytes were isolated and extracts of whole lymphocytes (T+B+K) and pure T lymphocytes were prepared. Whole lymphocyte extracts were further fractionated into fraction A containing mainly antibody and fraction B containing mainly membrane-bound receptors. The ability of various lymphocytes and their extracts to lyse allogeneic colonic epithelial cells in vitro was tested with and without the addition of 50 microgram of Common antigen (C.A.) used as a blocking agent. The percentage of cells lysed by whole lymphocytes correlated well with the severity of symptoms of the patients with U.C. Pure T lymphocytes were cytotoxic only in C.D. but not in U.C. Isolated extracts fractions A and B were not cytotoxic in active U.C. except when tested in combination. The addition of the Kunin antigen blocked the effect of lymphocytes from patients with active U.C. The results indicate differences in the mechanism of immunological activity of lymphocytes in U.C. and C.D. and show a direct relation of B lymphocytes and K cells to the cytotoxicity of U.C.

B-Lymphocytes↗
PubMedDate not supplied

Delayed type allergy in patients with ulcerative colitis. III. Allergic reactions evoked in colonic mucosa by autologous leukocytes and their extracts.

In 36 patients with ulcerative colitis hypersensitivity reactions induced in the colonic mucosa by autologous leukocytes and their extracts were studied. In 77% of cases reactions developed at the site of leukocyte injection and in 69% of cases at the site of leukocyte extra injection. The time of reaction appearance and the character of histological changes suggest that the reactions were of the cell-mediated type. The results of the investigations indicate that cell-mediated reactions may be an important pathogenic factor in ulcerative colitis.

Adult↗