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Biomedical subjects

R Vogt

Publications and source records attributed to R Vogt.

16 recordsLinked to original sources
PubMed1992

CD20 and CD5 expression in B-chronic lymphocytic leukemia.

In order to quantitate a previously noted decrease in CD20 fluorescence intensity (FI) on B-CLL lymphocytes, binding capacities [BC x 10(3) +/- 1SD = number of antibodies bound per cell] were calculated. The mean (N = 5) BC x 10(3) +/- 1SD of CD20 reagents for normal B-PBL and B-CLL lymphocytes confirmed this observation. B-PBL and B-CLL were 56 +/- 11 and 61 +/- 14, and 19 +/- 15 and 18 +/- 16, respectively, for Leu 16 and B1. Although adequate compensation standards for the determination of CD5 and CD20 coexpression are not available, qualitatively, the density of CD5 on both normal B-PBL and B-CLL is less compared to the expression of CD5 by normal T cells. CD5 expression on B-CLL seems to be linked to the lower levels of CD20, whereas CD5 expression may appear to be absent on CLL lymphocytes expressing normal levels of CD20. Levels of CD20 in B-CLL suggest involvement of one or two genes (alleles) whose decreased expression may be linked to CD5 expression.

Antigens, CD↗
PubMed1992

Flow cytometric analysis of whole blood lysis, three anticoagulants, and five cell preparations.

We studied the effects of anticoagulants and cell preparation methods on lymphocyte forward-angle scatter (FSC), autofluorescence, and immunofluorescent staining for CD45, CD14, and CD13. Blood samples collected in ethylenediaminetetracetic acid (EDTA), heparin, and acid citrate dextrose (ACD) were processed by using conventional Hypaque-Ficoll (HF) separation and four whole blood (WB) lysis techniques: Immuno-lyse, Q-Prep, FACS Lyse, and Gen Trak Lysis. Lymphocytes prepared by using three of the four whole blood methods gave FCS values comparable to those isolated by HF, while one method (FACS Lyse) gave consistently lower values. Autofluorescence values were comparable by all methods except Immuno-lyse, which showed consistently higher values in blood stored for 24 h with any anticoagulant. Immunofluorescent values for CD45-stained cells were quite consistent across all methods, and among the whole blood methods, FACS Lyse and Q-Prep uniformly gave the highest purity of CD45-positive cells in the lymphocyte light scatter gates. Additionally, propidium iodide (PI) analyses of CD45-stained whole blood, and analyzed without lysis, confirmed that ACD and heparin were superior to EDTA for maintaining viable leucocytes overnight. Future studies should focus on other commonly used reagents, a wide variety of abnormal samples, and cell viability.

Antigens, CD↗
PubMed1992

Collagel--a new viscoelastic substance for ophthalmic surgery.

A clinical trial comparing the viscoelastic properties of Collagel (human collagen) and Healon (sodium hyaluronate) was performed. Collagel was well tolerated and produced no untoward reaction in the eye. Flare cell measurement was performed during the seven day postoperative period. The initial peak and decline of flare and cell determination showed a similar course in groups treated with Collagel or Healon. A transient increase in intraocular pressure was seen. Endothelial cell loss following surgery was similar in the Collagel and Healon groups. There were no significant differences in intraocular pressure, corneal thickness, endothelial cell density, or visual acuity during the postoperative period. These results suggest that this new viscoelastic substance is comparable to Healon in respect to the parameters described.

Aged↗
PubMed1992

[Current comments on Freud's treatment of "The question of lay analysis"].

The author gives a review on Freud's basic thoughts on lay analysis from 1926 and proceeds with the question why the Deutsche Psychoanalytische Vereinigung (German Psychoanalytic Association) admits only such candidates for psychoanalytic training who are physicians or psychologists. This corresponds with the policies of German health insurance agencies, which include only physicians directly or psychologists indirectly in their plans. He suggests that this attitude derives from a limited view of psychoanalysis, for which he names three different reasons: historical, institutional, and those that grow out of the specifics of psychoanalytic practice.

Freudian Theory↗
PubMed1990

Implantation of posterior chamber lenses in eyes with phakodonesis and lens subluxation.

Unilateral posterior chamber intraocular lens (IOL) implantation was performed after phacoemulsification or extracapsular cataract extraction in ten of 11 patients with phakodonesis or lens subluxation, which was bilateral in two cases. To facilitate placement of the IOL, the anterior capsule was opened with a YAG laser in nine patients. Intraoperative vitrectomy was performed in two patients, and posterior chamber IOL implantation was accomplished with fixation in the ciliary sulcus. Postoperative follow-up studies failed to demonstrate significant decentration or dislocation of the IOLs. Phakodonesis and lens subluxation are no longer considered absolute contraindications to implantation of posterior chamber IOLs.

Aged↗
PubMed1990

["Archaic matrix of the oedipus complex"].

The clinical implication of Janine Chasseguet-Smirgel's concept of the "archaic matrix of the Oedipus complex" is examined, the resulting deeper understanding of the Oedipus myth is considered. A discussion follows of Chasseguet-Smirgel's way of using this concept in her historical evaluation of German Romantism with regard to the Nazi times and nowadays politics of the Westgerman Green Party.

Germany↗
PubMed1979

[Accuracy of liver scintigraphy in focal liver disease; a comparison with postmortem studies in 159 cases (author's transl)].

In recent literature numerous papers have been published concerning the accuracy of scintigraphic detection of liver metastases. Unfortunately however, the problem of false positive results is not particularly discussed in these papers. Because of the lack of information it was our aim to compare our own scintigraphic results with postmortem histopathological findings. Our investigations were carried out in 139 patients with various types of malignancy. Included in the investigations were 20 patients with primary liver tumor. The interval between scintigraphic examination and the histological verification ranged from 3 days to 1 year. In 62 of the patients with liver metastases, histopathology revealed liver metastases, while 77 patients showed no liver involvement. We arrived at the correct diagnosis "liver metastasis" in 50 out of 62 patients (80.6%). False negative scintigrams (19.4%) were found in most of the respective cases when diffuse malignant involvement such as leukemia and Hodgkin's disease was present, and also when the size of the metastases was less than 2 cm in diameter. Fifty six out of 77 patients (72.7%) without histopathological evidence of liver metastases revealed negative scintigrams. Twenty one (27.3%) false positive scintigrams were mostly due to (diffuse) nonmalignant disease e.g. fibrosis and cirrhosis. The overall accuracy of liver scintigraphy in our study was 76.2%. In 18 of 20 (90%) patients with focal liver disease correct diagnosis was established. 7 patients with benign liver tumors and 11 of 13 patients with hepatocellular carcinoma showed focal defects. Considering the fact that liver scintigraphy is a non-invasive procedure, it can be recommended as screening method. In connection with sonography and computer tomography liver scintigraphy can undoubtedly improve the diagnostic accuracy in detecting liver metastases and primary liver tumors.

Autopsy↗
PubMed1979

[Tumor localization in vivo on the basis of scintigraphic detection of clostridium rods by means of 131J-labeled antibodies and F(ab')2 antibody fragments].

The authors report about investigations for localization of tumours in vivo by scintigrafic iddntification of Clostridium butyricum Jena H8 by 131I-labelled antibodies and by 131I-labelled F(ab')2-fragment antibodies in mouse transplantation-tumour UVT 15264. The application of 131I-labelled anti-clostridial-rod-antibodies in tumour bearing mice demonstrates increase of radioactivity in tumours of animals, pretreated by spores, as well as in animals without pretreatment. These appearently unspecific increases of radioactivity could not be demonstrated by application of 131I-labelled F(ab')2-fragment antibodies directed against clostridial rods, but also the specific fixation of 131I-labelled F(ab')2-fragment antibodies in tumours of mice, pretreated by clostridial spores, could not be demonstrated. The results are discussed with regard to further investigations.

Animals↗
PubMed1978

The influence of vitamin B-6 on cystathioninuria in premature infants.

The amount of cystathionine excreted in the urine of premature infants, ages 10 to 17 days, decreases after oral administration of 40 mg vitamin B-6 daily. Thus, it can be speculated that in vivo cystathionase of premature infants may be induced or activated by pyridoxine.

Birth Weight↗
PubMedDate not supplied

[The prospective image the psychotherapist holds of his patient and its effect on the therapeutic process].

Psychonalytic theory of the therapeutic process is predominantly determined by the concepts of transference, resistance and countertransference. Decisive through the insight into the relationship between analyst and patient thus derived may be, the range of, potential meanings of these concepts does not in the first place encompass a complete theory of the therapist-patient-relationship. It mainly indicates how to use the therapeutical techniques which in turn define the theoretical scope of these terms. What is called the real aspects in the therapeutic relation, ie, those relatively free from transference, and their influence on the therapeutical process are so far unterrepresented in the theory of psychoanalysis. Yet the prospective view the therapist holds of his patient most probably has a decisive impact on the course of a treatment. Although the notion of countertransference offers some technical possibilities for correcting this image and for adjusting it to reality it is not sufficient to account for the complexity of the process in the psychoanalytic theory. The real features of the therapeutical relationship are the secret condition, the very background which alone allows the phenomena of transference and counter-transference to be detected as such.

Countertransference↗