PubMed Health⌕ Search

Biomedical subjects

R Webster

Publications and source records attributed to R Webster.

At least 55 records · Page 3Linked to original sources
PubMed1982

Effect of monoclonal anti-neuraminidase antibodies on the kinetic behavior of influenza virus neuraminidase.

Neuraminidase from the recombinant influenza virus A/NWSHA-Tokyo/3/67NA HON2 has been shown to exhibit non-Michaelis-Menten kinetics. The multiphasic behaviour was demonstrated for both the isolated neuraminidase heads and for the intact virus. Interaction of the enzyme with two monoclonal anti-neuraminidase antibodies (WANA 1 and RANA 1), which recognize separate antigenic determinants on the molecule, resulted in hyperbolic kinetic behaviour. While both antibodies abolished the multiphasic kinetics of the enzymic reaction, only WANA 1 altered the Vmax and Km values, indicating that it may in some way inhibit the interaction of enzyme and substrate.

Antibodies, Monoclonal↗
PubMed1981

Antigenic structure of influenza virus haemagglutinin defined by hybridoma antibodies.

The recurrence of influenza virus infection in man is attributed primarily to changes occurring in the antigenic structure of the viral surface glycoproteins, especially of the haemagglutinin (HA) molecule. Comparative antigenic analysis of epidemic influenza virus strains has allowed the description of 'strain-specific' and 'cross-reactive' antigenic determinants. However, the interpretation of these findings remained ambiguous, because the specificity of the applied antisera was insufficiently defined and because the antigenic differences among the HA molecules of various epidemic virus strains resulted presumably from a large number of amino acid substitutions. Thus, in characterizing the antigenic structure of the HA molecule, our approach has been (1) to generate a panel of monoclonal anti-HA hybridoma antibodies, (2) to use some of these antibodies to select mutants of the influenza A/PR/8/34 (PR8) virus expressing antigenically altered HA molecules, and (3) to construct an operational antigenic map of the HA molecule by comparative antigenic analysis of the mutant viruses with the monoclonal antibodies. As we report here, analysis of the 34 mutant viruses selected has enabled us to define four antigenic sites on the HA molecule. Our observation that these sites have undergone antigenic drift to a different extent in nature implies that the mechanisms responsible for antigenic drift act selectively on distinct structures of the HA molecule.

Animals↗
PubMed1980

Experience with a general practice elective in the undergraduate curriculum.

The eight-week general practice elective at the University of Melbourne, after the introduction of a half-year of electives into the sixth year of the medical course, is here described. Seventy students and 50 clinical instructors recorded their opinions, which were generally strongly in favour of the semistructured course. Students were undoubtedly impressed by the skill of the general practitioner in diagnosis and treatment of a wide variety of illnesses. The majority of students found the period to be an interesting and rewarding educational experience, and many stated that it altered favourably their view of general practice. The impact of this course is discussed in the context of future changes in medical education.

Australia↗
PubMed1979

Undergraduate teaching in primary medical care.

There is considerable variation between medical schools both in the timing of undergraduate teaching of general practice and in the methods of teaching. In 1976, the Faculty of Medicine in the University of Melbourne determined that the period of exposure of students to teaching primary medical care would be two weeks in the fourth year, and an optional eight weeks in the sixth year. This paper describes the fourth year teaching programme and its assessment by the students. Essentially, it consists of a two week course of clinical general practice in which an attempt is made to integrate the knowledge and experience gained from both hospital and community medicine.

Australia↗
PubMed1977

Community health in the University of Melbourne.

After changes to the curriculum of the University of Melbourne Medical School, a Department of Community Health has been established. Undergraduate teaching will be carried out in the first, third, fourth and sixth years of the course. Although an introduction to primary care will occur in first year, the teaching emphasis in the preclinical years will be in the behavioural sciences, epidemiology and social medicine. Primary care as a clinical subject will be taught in fourth year with electives available in the sixth year.

Australia↗
PubMed1977

Inhibition of amino acyl tRNA synthetase activity by copper complexes of two metal binding ligands. N-Methyl isatin beta-thiosemicarbazone and 8-hydroxyquinoline.

Copper complexes of N-methyl isatin beta-thiosemicarbazone, 1-formyl isoquinoline thiosemicarbazone and thiosemicarbazide inhibit amino acyl tRNA synthetase activity. Copper complexes of 8-hydroxyquinoline and 8-mercaptoquinoline also inhibit. The 1 : 1 ligand-metal complex is significantly more active than the 2 : 1 complex. The free ligand alone and copper sulfate alone have little, if any, effect. These complexes have no effect on the ATP-PPi exchange reaction and do not cause deacylation of amino acyl tRNAs. This indicates that the process inhibited by these complexes is the amino acylation reaction. This is the first report that these copper binding ligands can inhibit enzymatic processes which involve nucleic acids but which are not viral, bacterial or mammalian cell polymerases.

Amino Acyl-tRNA Synthetases↗
PubMedDate not supplied

The importance of marketing in nurse education.

There can be little doubt that changes in the National Health Service (NHS) heralded by the 1989 Government White Paper, Working for Patients, have significant implications for nurse education. Not least will be the need for Colleges and Schools of Nursing to present a high profile in terms of the services they offer. This paper explores the concept of marketing and its increasing importance to nurse education. It examines Giles' three propositions in relation to marketing, and suggests that these may be applied successfully to organisations providing a service, as well as those producing material goods. It looks at how and why marketing is necessary to nurse education, and suggests that marketing is an essential tool in assisting the School to achieve its objectives. Marketing strategies are discussed in detail, looking first at methods of research, then at the processes used to sell the courses being offered. These include the techniques of developing the offering, marketing the offering, facilitation, valuation and finally, promotional communication. The paper concludes by summarising the reasons why marketing techniques will be essential to the future success of nurse education, at a time when it is so vital to ensure that a well qualified nursing workforce is prepared to meet the challenges of the future.

Advertising↗
PubMedDate not supplied

Germ of an idea.

Explore the source record for details and available documents.

Aerosols↗
PubMedDate not supplied

Prevalence of psychotic symptoms in delirium.

Psychosis in delirium has been an underresearched area. The authors retrospectively examined the prevalence of psychotic symptoms and possible associated factors in the records of 227 consecutive hospitalized patients. These patients had been diagnosed with delirium, according to the DSM-IV criteria and referred to a psychiatry consult service. The authors compared patients, with or without psychosis, on demographic variables, medical and psychiatric history; number of medications, etiology of delirium, and cognitive state. The prevalence of psychotic symptoms was 42.7% (n = 97) with 27% of patients (n = 61) having visual hallucinations, 12.4% (n = 28) having auditory hallucinations, 2.7% (n = 6) having tactile hallucinations, and 25.6% (n = 58) having delusions. The presence of visual hallucinations, but not delusions or auditory hallucinations, was significantly associated with more active medical diagnoses and multiple etiologies causing the delirium. Psychotic symptoms are not uncommon in delirium, but specific psychotic symptoms may have different factors contributing to their development. Visual hallucinations appear to be associated with a greater number of active medical disorders, but other factors associated with the development of psychotic symptoms in delirium are currently unknown.

Adult↗
PubMedDate not supplied

Palliative care for cancer patients.

To improve the quality of palliative care, we recommend the establishment, in larger hospitals, of regionalized units with medical directors. The current concept that the acute hospital is not appropriate for the admission of the terminally ill is rejected as is the need for the establishment of further free-standing hospices.

Australia↗