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Biomedical subjects

R Yanagawa

Publications and source records attributed to R Yanagawa.

At least 145 records · Page 8Linked to original sources
PubMed1975

Possible role of protein(s) as antigenic determinant of the type-specific main antigen of Leptospira kremastos strain Kyoto.

Acid hydrolysates of the type-specific main (TM) antigen isolated from Leptospira kremastos strain Kyoto showed a considerable inhibition of complement fixation reaction between TM antigen and anti-strain Kyoto serum. A fraction of 2 N HCl hydrolysate containing exclusively a large amount of neutral sugars showed no inhibition, whereas another fraction containing only a small amount of protein(s) showed a slight inhibition. The remaining fractions containing amino sugars, protein(s), and neutral sugars showed a slight inhibition, but the inhibition did not correlate with the amount of amino sugars. TM antigen, delipidized by hydrolysis with 1 N HCl for 4 min and then dialysis, showed a strong inhibition. The lipid fraction did not show any inhibition. The inhibitory effect of nondialyzable delipidized TM antigen was completely lost by treating the antigen with proteolytic enzymes, indicating that the antigenic determinant of L. kremastos strain Kyoto is intimately related with protein(s).

Antigens, Bacterial↗
PubMed1975

Failure of transformation in leptospira with two genetic markers of streptomycin resistance and the ability to grow in a synthetic medium.

Transformability of Leptospira was investigated with the two genetic markers, streptomycin (SM) resistance and the ability to grow in SHENBERG's synthetic medium. DNA was extracted from the two mutants of L. icterohaemorrhagiae strain Shibaura, which were SM-resistant and able to grow in SHENBERG's medium. Recipients were 5 strains of L. icterohaemorrhagiae and 1 strain of L. copenhageni. Studies taking careful consideration of the growth phase of the recipient cells, transformation medium, and incubation temperature did not produce the appearance of transformant. Transformation of L. kremastos with SM resistance also showed a negative result.

Culture Media↗
PubMed1975

Distribution of Corynebacterium renale among healthy bulls with special reference to inhabitation of type III in the prepuce.

Distribution of Corynebacterium renale among apparently healthy bulls reared in Hokkaido was investigated. The organism was detected from 46 (39.3%) of 117 specimens of preputial cavity washing and from 60 (51.7%) of 116 specimens of semen. The isolates studied in this survey belonged to type III, except a few which belonged to type II. No type I strain was isolated from any bull. C. renale type III was isolated from the prepuce in six of seven bulls slaughtered and from urethra in three, but not at all from any other organ. In the seven bulls, no macroscopic changes were seen, but a slight infiltration of lymphocytes and formation of lymph nodules were noticed in the prepuce. No other microscopical changes could be demonstrated in any other organ. No serum antibody response was detected. To ascertain the virulence of C. renale isolated from the bulls, a strain of type II was inoculated into the urinary bladder of a healthy cow. The cow exhibited fever and hematuria on and after the 10th day. Typical cystitis was proved when the cow was necropsied on the 14th day after inoculation. From these result it is conceived that C. renale type II organisms inhabit the prepuce of apparently healthy bulls at a high rate, without inducing any disturbance.

Animals↗
PubMed1975

Attachment of Corynebacterium renale to tissue culture cells by the pili.

One or more cells of Corynebacterium renale strains (serologic types, I, II and III), which possessed numerous pili, frequently were attached to BHK-21 cells, primary dog kidney cells, and primary rabbit kidney cells. The percentage of the cultured cells to which C renal cells were attached was about 70%. The percentage was less with cells of C renale possessing fewer pili, around 30%. After C renale was treated with the homologous anti-pili serum, the percentage of BHK-21 cells to which bacterial cells were attached was even less (22%). In electron micrographs, the pili of C renale were observed to attach themselves to the membranes of BHK-21 cells. The adhesive property of the pili of C renale to tissue culture cells was thus demonstrated.

Cells, Cultured↗
PubMed1975

Immunizing effects of structural components of Leptospira icterohaemorrhagiae.

The protective attributes of the structural components of Leptospira icterohaemorrhagiae strain Shibaura were examined. Guinea pigs were immunized with the outer envelope, the cell wall, the type-specific main antigen, and the protein constituent of the outer envelope respectively. Of the 4 preparations, the outer envelope, the cell wall and the type-specific main antigen showed a considerable protective effect in this order. The protein constituent of the outer envelope was less effective. Immunization with 200 mug of the outer envelope protected guinea pigs from lethal infection but no completely from the renal carrier state. The rate of protection from renal leptospirosis by the preparations of leptospiral components and lyophilized whole cells was always inferior to the survivor rate.

Animals↗
PubMed1974

Agglutination of trypsinized sheep erythrocytes by the pili of Corynebacterium renale.

Corynebacterium renale strains which possessed numerous pili agglutinated trypsinized sheep erythrocytes, whereas those possessing only a few pili did not show such activity. By electron microscopy, the pili of C. renale adhered to the ghost of trypsinized sheep erythrocytes. After the treatment of C. renale with the homologous anti-pili serum, neither the agglutination of trypsinized sheep erythrocytes nor the adherence to the ghost by the pili were found. The adhesive property of the pili of C. renale was thus demonstrated.

Absorption↗