Biomedical subjects

Richard Wells

Publications and source records attributed to Richard Wells.

9 recordsLinked to original sources

PubMed2005

Comparison of measurement methods for quantifying hand force.

Hand force is a known risk factor for upper extremity disorders. The objective of the present study was to determine the characteristics of, and the relationships between, exposure assessment methods to quantify hand force. Five methods, used in the laboratory or the field, were used to quantify hand force at three force magnitudes: two direct (or technical) measurement methods, force transducers and electromyography; an observational method; and two self-report approaches, force matching and a visual analogue scale. Five tasks, simulating manual work activities, were performed by 20 participants. The coefficients of variation of measures within and between participants were moderate. All approaches clearly distinguished between the three force levels tested. The reliability of the methods ranged from poor (observation method without information) to good (force transducers method and observation method with information). The measurement methods correlated moderately over all five tasks. Predictions of grip force across all five tasks were poor and even for single tasks the predictions were not much better. The tasks in this study were still simplified; in the field tasks are even more complex and the measurement characteristics might be expected to be less good. A hand force exposure assessment method should therefore be calibrated and tested for each type of hand activity before use.

Adolescent↗

PubMed2005

Effect of cycle time and duty cycle on psychophysically determined acceptable levels in a highly repetitive task.

Psychophysical methodology has been used to develop guidelines for lifting and more recently similar methods have been applied to repetitive upper limb movements. While a range of cycle times are usually used, there is often no control for duty cycle. The purpose of this paper is to present psychophysically determined acceptable torques for a common upper limb task, with both cycle time and duty cycle conditions set by the researcher. Eight female participants, sitting at adjustable workstations, performed a simulated in-line screw running task. A computer-controlled torque motor applied a torque every 3, 6, 12 or 20 s with a duty cycle of 25, 50 or 83%. The participants worked with one set of conditions each day and self-selected the highest torque that they felt was acceptable without developing undue pain and discomfort. Duty cycle was found to significantly affect the amount of torque selected. With duty cycle controlled, cycle time was no longer found to have any significant effect on selected torque. Acceptable torques for 25, 50 and 83% duty cycles were 1.09, 0.9 and 0.73 Nm. Discomfort and stiffness were concentrated on the back of the hand and on the thumb web. These findings suggest that increased perception of discomfort with increased frequency (decreased cycle time) may be related to decreased rest/recovery time for muscles.

Adult↗

PubMed2005

Disease biology rather than age is the most important determinant of survival of patients > or = 60 years with acute myeloid leukemia treated with uniform intensive therapy.

BACKGROUND: The objectives of the current study were to evaluate the outcome of patients > or = 60 years with acute myeloid leukemia (AML) treated uniformly with high-dose daunorubicin containing induction and modified high-dose cytosine arabinoside containing postremission therapy, and to identify factors predictive of complete disease remission (CR) and survival. METHODS: Between 1998 and 2002, the authors treated 117 newly diagnosed patients (acute promyelocytic leukemia excluded) with AML > or = 60 years (median, 67 years; range, 60-82 years). Karyotype (Medical Research Council classification) at diagnosis was categorized as good risk (n = 3), intermediate risk (n = 69), adverse risk (n = 26), and suboptimal/not done (n = 19). A normal karyotype was seen in 41 patients and 40 (34%) had secondary AML. RESULTS: The outcome of induction included the following: CR, 62 (53%); early death, 5 (4%); death during hypoplasia, 14 (12%); and resistant disease, 36 (31%). The 3-year event-free (EFS) and overall survival (OS) rates were 9% (95% confidence interval [95% CI], 3-16%) and 17% (95% CI, 9-29%), respectively. In a univariate analysis, cytogenetics, lactate dehydrogenase level, leukocyte count, and performance status were the significant factors for EFS and OS. Age was not a significant prognostic factor for either CR or survival. In a multivariate model, adverse-risk cytogenetics, previous history of myelodysplastic syndrome or antecedent hematologic disorder, and high leukocyte count (> 30 x 10(9)/L) were independent adverse prognostic factors for survival. The impact of adverse karyotype on EFS and OS was time dependent and was observed after 50 and 150 days, respectively. CONCLUSIONS: The authors concluded that candidacy for intensive therapy in older patients should be based on biologic features of disease and fitness, rather than on age.

Acute Disease↗

PubMed2005

Training the next generation of researchers in work disability prevention: the Canadian Work Disability Prevention CIHR Strategic Training Program.

INTRODUCTION: There is a need to create, disseminate, and implement new knowledge in the work disability prevention (WDP) field. Training programs attracting high-quality applicants and taking into account the complexity of this emerging field are urgently needed. METHODS: An advanced training program, funded by the Canadian Institutes of Health Research (CIHR), was developed by 24 mentors affiliated with nine different universities. The main objective of this program is to develop transdisciplinary knowledge, skills, and attitudes regarding WDP. This program has been developed for PhD students or post-doctoral fellows already registered full-time in a Canadian or recognized foreign university whose main interest is WDP, regardless of the health problem. RESULTS: Since its implementation, the program received two successive cohorts of 10 students. They were registered in 13 universities in five countries and trained in nine different disciplines. CONCLUSIONS AND SIGNIFICANCES: Appropriate WDP research may save major societal costs attributable to prolonged work disability. The proposed training program will contribute to developing tomorrow's research workforce.

Attitude of Health Personnel↗

PubMed2005

Molecular characterization of salivary gland malignancy using the Smgb-Tag transgenic mouse model.

The molecular mechanisms underlying salivary gland tumorigenesis remain unclear. In order to identify genetic changes that occur during the development of invasive adenocarcinoma from normal salivary gland, we used the Smgb-Tag transgenic mouse model. This transgene induces the progressive development of dysplasia to invasive adenocarcinoma in the submandibular salivary gland. Gene expression patterns from 20 submandibular glands (two normal, nine dysplasia and nine adenocarcinoma samples) were assessed using a mouse 15 K cDNA array. Unsupervised hierarchical clustering was used to group gene expression based on 157 differentially expressed genes distinguishing between dysplasias and adenocarcinomas. Further analysis identified 25 significantly overexpressed and 28 underexpressed cDNA sequences in adenocarcinoma as compared to dysplasia. Differential expression of five genes (Lcn2, Ptn, Cd24a, Mapk6 and Rnps1) was validated by quantitative real-time RT-PCR in a total of 48 mouse salivary gland tissues (seven histologically normal, 13 dysplasias and 28 adenocarcinomas), including the 20 samples analyzed by cDNA arrays. Immunohistochemical analysis was used to validate the expression of Ptn and Cd24a at the protein level in a subset of 16 mouse salivary glands (four normal, five dysplasia and seven adenocarcinoma samples), as well as in 23 human submandibular gland tumors (16 pleomorphic adenomas, three adenoid cystic carcinomas, one acinic cell carcinoma, one adenocarcinoma NOS, one myoepithelial and one mucoepidermoid carcinoma). We thus demonstrated that the Smgb-Tag transgenic mouse model is a useful tool for the identification of genes that are deregulated in salivary gland adenocarcinomas. Our data suggest that Ptn and Cd24a may be genetic markers associated with salivary gland tumorigenesis and/or progression.

Adenocarcinoma↗

PubMed2004

Measurement of prehensile grasp capabilities by a force and moment wrench: methodological development and assessment of manual workers.

Prehensile grasp capability is typically quantified by pinch and grasp forces. This work was undertaken to develop a methodology to assess complex, multi-axis hand exertions through the measurement of forces and moments exerted by the hand along and about three orthogonal axes originating at the grip centre; termed an external wrench. Instrumentation consisting of a modified pinch/grip dynamometer affixed to a 6 df force cube was developed to simultaneously measure three forces, three moments and the pinch/grip force about the centre of the grip. Twenty right hand dominant manual workers (10 male and 10 female), free of hand or wrist disorders, completed a variety of maximal strength tasks. The randomized block design involved three separate grips--power grip, lateral pinch and pulp pinch. Randomized within each block were three non-concurrent repetitions of isolated maximal force and moment generations along and about the three principle orthogonal axes and a maximal grip force exertion. Trials were completed while standing, with the arm abducted and elbow flexed to 90 degrees with a wrist posture near neutral. Where comparable protocols existed in the literature, forces and moments exerted were found to be of similar magnitude to those reported previously. Female and male grip strengths on a Jamar dynamometer were 302.6 N and 450.5 N, respectively. Moment exertions in a power grip (female and male) were 4.7 Nm and 8.1 Nm for pronator, 4.9 Nm and 8.0 Nm for supinator, 6.2 Nm and 10.3 Nm for radial deviator, 7.7 Nm and 13.0 Nm for ulnar deviator, 6.2 Nm and 8.2 Nm for extensor, and 7.1 Nm and 9.3 Nm for flexor moments. Correlations with and between maximal force and moment exertions were only moderate. This paper describes instrumentation that allows comprehensive characterization of prehensile force and moment capability.

Biomechanical Phenomena↗

PubMed2004

Mechanical exposure concepts using force as the agent.

This paper presents a model that addresses mechanical exposure with regard to the development of musculoskeletal disorders, defines exposure concepts, unifies a variety of exposures, and includes the concept of human activity. When force is used as an agent, concepts related to the measurement, transformation, and interaction of the agent with tissues can be developed for use in epidemiologic exposure assessment and hazard assessment. The importance of tissue response in the exposure modeling process and in the creation of exposure indices is highlighted. Unfortunately, the response of tissue to forces of varying amplitudes and time variation patterns are largely unknown and thus reduce the possibility to develop optimal exposure assessment metrics. Although the paper argues that an exposure index at the tissue level may be the most powerful, considerations of resources and current knowledge make exposure indices based on external exposure or internal exposure preferable choices.

Biomechanical Phenomena↗

PubMed2002

Measurement of microbial activity in soil by colorimetric observation of in situ dye reduction: an approach to detection of extraterrestrial life.

BACKGROUND: Detecting microbial life in extraterrestrial locations is a goal of space exploration because of ecological and health concerns about possible contamination of other planets with earthly organisms, and vice versa. Previously we suggested a method for life detection based on the fact that living entities require a continual input of energy accessed through coupled oxidations and reductions (an electron transport chain). We demonstrated using earthly soils that the identification of extracted components of electron transport chains is useful for remote detection of a chemical signature of life. The instrument package developed used supercritical carbon dioxide for soil extraction, followed by chromatography or electrophoresis to separate extracted compounds, with final detection by voltammetry and tandem mass-spectrometry. RESULTS: Here we used Earth-derived soils to develop a related life detection system based on direct observation of a biological redox signature. We measured the ability of soil microbial communities to reduce artificial electron acceptors. Living organisms in pure culture and those naturally found in soil were shown to reduce 2,3-dichlorophenol indophenol (DCIP) and the tetrazolium dye 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt (XTT). Uninoculated or sterilized controls did not reduce the dyes. A soil from Antarctica that was determined by chemical signature and DNA analysis to be sterile also did not reduce the dyes. CONCLUSION: Observation of dye reduction, supplemented with extraction and identification of only a few specific signature redox-active biochemicals such as porphyrins or quinones, provides a simplified means to detect a signature of life in the soils of other planets or their moons.

Bacteria↗

PubMed2002

Supercritical fluid extraction and high-performance liquid chromatography-diode array-electrochemical detection of signature redox compounds from sand and soil samples.

A supercritical fluid extraction procedure and a chromatographic separation/detection method were developed for the detection of Earth-based microorganisms. After microbes in a sand or a soil sample were hydrolyzed in a diluted NH(4)OH/acetone solution, several redox compounds from bacteria could be effectively extracted with trimethylamine-modified supercritical CO(2) at 35 degrees C and 300 atm. These signature redox-active compounds were separated by a reversed-phase HPLC column in an ion-pair mode and then monitored with a diode array detector and an electrochemical detector. The analytical results demonstrated the feasibility of using the reported techniques to detect the chemical signature of life in barren desert sand samples.

Bacteria↗