Supercritical fluid extraction and high-performance liquid chromatography-diode array-electrochemical detection of signature redox compounds from sand and soil samples.

Qingyong Lang; I Francis Cheng; Chien M Wai; Andrzej Paszczynski; Ronald L Crawford; Bruce Barnes; Tony J Anderson; Richard Wells; Giancarlo Corti; Lisa Allenbach; Daniel P Erwin; Touraj Assefi; Mohammad Mojarradi

doi:10.1006/abio.2001.5502

PubMed · 11814293

Supercritical fluid extraction and high-performance liquid chromatography-diode array-electrochemical detection of signature redox compounds from sand and soil samples.

Qingyong Lang·I Francis Cheng·Chien M Wai·Andrzej Paszczynski·Ronald L Crawford·Bruce Barnes·Tony J Anderson·Richard Wells·Giancarlo Corti·Lisa Allenbach·Daniel P Erwin·Touraj Assefi·Mohammad Mojarradi

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Abstract

A supercritical fluid extraction procedure and a chromatographic separation/detection method were developed for the detection of Earth-based microorganisms. After microbes in a sand or a soil sample were hydrolyzed in a diluted NH(4)OH/acetone solution, several redox compounds from bacteria could be effectively extracted with trimethylamine-modified supercritical CO(2) at 35 degrees C and 300 atm. These signature redox-active compounds were separated by a reversed-phase HPLC column in an ion-pair mode and then monitored with a diode array detector and an electrochemical detector. The analytical results demonstrated the feasibility of using the reported techniques to detect the chemical signature of life in barren desert sand samples.

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Bacteria Chromatography, High Pressure Liquid Chromatography, Supercritical Fluid Electrochemistry Flavin Mononucleotide Flavin-Adenine Dinucleotide Hemin Oxidation-Reduction Riboflavin Silicon Dioxide Soil Ubiquinone Journal Article Research Support, U.S. Gov't, Non-P.H.S.

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Qingyong Lang, I Francis Cheng, Chien M Wai, Andrzej Paszczynski, Ronald L Crawford, Bruce Barnes, Tony J Anderson, Richard Wells, Giancarlo Corti, Lisa Allenbach, Daniel P Erwin, Touraj Assefi, Mohammad Mojarradi. 2002-02-15. Supercritical fluid extraction and high-performance liquid chromatography-diode array-electrochemical detection of signature redox compounds from sand and soil samples.. https://doi.org/10.1006/abio.2001.5502

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