Biomedical subjects

Ro Dong Park

Publications and source records attributed to Ro Dong Park.

4 recordsLinked to original sources

PubMed2005

Synergistic antiinflammatory effects of pinitol and glucosamine in rats.

This study evaluated the antiinflammatory activities of pinitol and glucosamine either alone or in combination against carrageenan- and cotton pellet-induced acute and subacute inflammation in rats. Five groups were included in each of the acute and subacute inflammation studies: the vehicle control group, positive control group (aminopyrine 100 mg/kg), pinitol group (20 mg/kg), glucosamine group (25 mg/kg) and a pinitol (20 mg/kg) and glucosamine (25 mg/kg) combination group. When 20 mg/kg of pinitol was administered to the rats, paw edema induced by the carrageenan injection was significantly suppressed and the level of granuloma formation induced by the cotton pellet implantation was slightly reduced. When 25 mg/kg of glucosamine was administered, paw edema caused by the acute inflammation was slightly reduced and the level of granuloma formation caused by the subacute inflammation was strongly suppressed. Although the combined application of pinitol and glucosamine did not have an additional antiinflammatory effect on the paw edema caused by acute inflammation, it did have an increased antiinflammatory effect on the formation of granuloma induced by subacute inflammation. Therefore, pinitol and glucosamine have an antiinflammatory effect on acute and subacute conditions. Moreover, a synergistic antiinflammatory effect against subacute inflammation was observed when the two chemicals were administered in combination.

Animals↗

PubMed2005

Anticoagulant 1,2,3,4,6-pentagalloyl-beta-D-glucopyranose isolated from geranium (Pelargonium inquinans Ait).

Geranium (Pelargonium inquinans Ait) leaves were extracted with 80% MeOH, and partitioned into n-hexane, ethyl acetate, BuOH and H2O to isolate the anticoagulant principles. The EtOAc fraction was found to be the most active, and was further purified using silica and octadecylsilane column chromatography employing a bioassay-guided fractionation method. The active compound was isolated and identified as 1,2,3,4,6-pentagalloyl-beta-D-glucopyranose (PGG) (compound I). The isolated anticoagulant significantly prolonged the activated partial thrombin time (APTT) and thrombin time (TT) using normal human plasma. One microgram of 1,2,3,4,6-pentagalloyl-beta-D-glucopyranose showed 0.063 heparin units in the APTT and 2.73 heparin units in the TT for anti-thrombosis. This is the first report of the isolation of PGG from geranium plants.

Anticoagulants↗

PubMed2005

Selective preparation of N-acetyl-D-glucosamine and N,N'-diacetylchitobiose from chitin using a crude enzyme preparation from Aeromonas sp.

A bacterium, Aeromonas sp. GJ-18, having strong chitinolytic activity was isolated from coastal soil and used for crude enzyme preparations. This enzyme preparation contained N-acetyl-D-glucosaminidase and N,N'-diacetylchitobiohydrolase. N-Acetyl-D-glucosaminidase was inactive above 50 degrees C, but N,N'-diacetylchitobiohydrolase was stable at this temperature. Utilizing the temperature sensitivities of the chitin degradation enzymes in crude enzyme preparation, N-acetyl-D-glucosamine (GlcNAc) and N,N'-diacetylchitobiose [(GlcNAc)(2)] were selectively produced from chitin. At 45 degrees C, GlcNAc was produced as a major hydrolytic product (94% composition) with a yield of 74% in 5 d, meanwhile at 55 degrees C (GlcNAc)(2) was the major product (86%) with a yield of 35% within 5 d.

Acetylglucosamine↗

PubMed2003

2-ketogluconic acid production and phosphate solubilization by Enterobacter intermedium.

Enterobacter intermedium, isolated from grass rhizosphere, exhibited a strong ability to solubilize insoluble phosphate. This bacterium oxidized glucose to gluconic acid and sequentially to 2-ketogluconic acid (2-KGA), which was identified using HPLC and GC-MS. The ability of E. intermedium to solubilize phosphate and produce 2-KGA produce in broth medium containing different components was monitored with air and without air supply. With an air supply, the production of 2-KGA markedly increased to about 110 g/l at day 10 in media containing 0.2 M gluconic acid, while it was about 65 g/l without gluconic acid addition. With an air supply, the concentration of soluble phosphate significantly decreased to 200-250 mg/l in media containing 1% CaCO3, whereas it was about 1000 mg/l without CaCO3 addition. Without an air supply, the concentration of 2-KGA and phosphate were negligible throughout the culture period.

Culture Media↗