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2-ketogluconic acid production and phosphate solubilization by Enterobacter intermedium.

Hoon Hwangbo·Ro Dong Park·Yong Woong Kim·Yo Sup Rim·Keun Hyung Park·Tae Hwan Kim·Jang Sun Suh·Kil Yong Kim
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Abstract

Enterobacter intermedium, isolated from grass rhizosphere, exhibited a strong ability to solubilize insoluble phosphate. This bacterium oxidized glucose to gluconic acid and sequentially to 2-ketogluconic acid (2-KGA), which was identified using HPLC and GC-MS. The ability of E. intermedium to solubilize phosphate and produce 2-KGA produce in broth medium containing different components was monitored with air and without air supply. With an air supply, the production of 2-KGA markedly increased to about 110 g/l at day 10 in media containing 0.2 M gluconic acid, while it was about 65 g/l without gluconic acid addition. With an air supply, the concentration of soluble phosphate significantly decreased to 200-250 mg/l in media containing 1% CaCO3, whereas it was about 1000 mg/l without CaCO3 addition. Without an air supply, the concentration of 2-KGA and phosphate were negligible throughout the culture period.

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Culture MediaEnterobacterGluconatesHydrogen-Ion ConcentrationPhosphatesPlant RootsPoaceaeSoil MicrobiologySolubilityJournal ArticleResearch Support, Non-U.S. Gov't

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Hoon Hwangbo, Ro Dong Park, Yong Woong Kim, Yo Sup Rim, Keun Hyung Park, Tae Hwan Kim, Jang Sun Suh, Kil Yong Kim. 2003. 2-ketogluconic acid production and phosphate solubilization by Enterobacter intermedium.. https://doi.org/10.1007/s00284-002-3951-y

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