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S A Gostimskiĭ

Publications and source records attributed to S A Gostimskiĭ.

11 recordsLinked to original sources
PubMed2005

[RAPD and ISSR analyses of regenerated pea Pisum sativum L. plants].

Long-term pea callus cultures of different genotypes (mutants R-9 and W-1 and cultivar Viola) were used to regenerate plants (generation R0). The regenerants displayed changes both in qualitative and in quantitative traits. The most dramatic morphological alterations and complete sterility were observed in regenerants of the cultivar Viola. To estimate the genetic differences, regenerants were compared with the original lines with the use of RAPD (random amplified polymorphic DNA) and ISSR (inter simple sequence repeat) analyses. The extent of divergence varied among regenerants and depended mostly on the original genotype. The genetic difference from the original line was no more than 1% in W-1 regenerants, 0.7-5.3% in R-9 regenerants, and 10-15% in sterile regenerants of the cultivar Viola. The genetic variation of plants regenerated from a callus culture maintained for ten years did not exceed that of plants obtained from a culture maintained for two years.

Base Sequence↗
PubMed2005

[Studying plant genome variation using molecular markers].

The authors' studies on the organization and variation of plant genome with the use of molecular markers are briefly reviewed with special emphasis on random amplified polymorphic DNA (RAPD), inter simple sequence repeat (ISSR), sequence characterized amplified region (SCAR), and cleaved amplified polymorphic sequence (CAPS) markers detected with the use of polymerase chain reaction (PCR). These markers have been demonstrated to be promising for identifying cultivars and determining the purity of genetic strains of pea. Genetic relationships between strains, cultivars, and mutants of pea have been studied. The role of molecular markers in molecular genetic mapping and localizing the genes of commercially important characters of pea has been shown. The possibility of the use of molecular markers for studying somaclonal variation and detecting mutagenic factors in plants during long-term spaceflights is considered. The prospects of using DNA markers for understanding the organization and variability of higher plant genomes are discussed.

Genetic Markers↗
PubMed2005

[Development and study of SCAR markers in pea (Pisum sativum L.)].

In order to develop more specific markers that characterize particular regions of the pea genome, the data on nucleotide sequences of RAPD fragments were used for choosing more extended primers, which may be helpful in amplifying a fragment corresponding to the particular DNA region. Of the 14 STS markers obtained from 14 polymorphic RAPD fragments, 12 were polymorphic, i.e., they are SCAR markers that can be used in genetic analysis. The transition from complex RAPD spectra to amplification of a particular SCAR marker substantially facilitates analysis of large samples for the presence or absence of the examined fragment. Inheritance of the developed SCAR markers was studied in F1 and F2. SCAR markers were used to identify various pea lines, cultivars, and mutants. It was established that the study of amplification of STS markers in various pea genotypes at varying temperatures of annealing and the comparison with amplification of the original RAPD fragments in the same genotypes provide an approach for analysis of RAPD polymorphism type.

DNA, Plant↗
PubMed2004

[Identification and mapping of chi115 gene and DNA markers linked to it in pea (Pisum sativum L.)].

Chlorophyll mutant Chi115 was induced by ethylmethane sulfonate (EMS) treatment of seeds of genotype Torsdag in Moscow State University and is characterized by lighter plant color. The monogenic nature of the mutant was determined by analyzing the F2 population from a cross between two P. sativum genotypes, WL1238 and Chi115. To establish a local map around the chi115 gene, the RAPD and ISSR techniques were used with 45 RAPD and 10 ISSR primers in combination with bulked segregant analysis (BSA). Linkage of 12 RAPDs and 2 ISSRs to the chi115 locus was observed in analysis of F2 single plants. Two RAPD markers that were closely associated with the chi115 gene were converted into the sequence characterized amplified region (SCAR) markers. By lowering the LOD score to 2, the linkage group containing the chi115 gene could be linked to the b gene (color of the flower) on linkage group III. Nevertheless, to prove the result obtained, three CAPS markers Sodmt, TubA1, and Rb were chosen on linkage group III. The results of linkage analysis showed that these CAPS markers were located within the linkage group including the chi115 gene.

Genes, Plant↗
PubMed2003

[Analysis of specific RAPD- and ISSR-fragments in somaclonal maize (Zea mays L.) and development of SCAR markers based on them].

RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter Simple Sequence Repeats) markers were used to analyse the genetic divergence between the regenerated plants derived from callus cultures and the original maize line A188. Analysis of polymorphism by using 38 RAPD- and 10 ISSR-oligonucleotide primers showed that the differences between eight examined somaclones and the original line ranged from 6.5 to 23%. As confirmed using new primers, the regenerants derived from callus cultures grouped into two clusters according to their origin. The regenerants isolated from calluses grown for eight months differed from one another and the original line to a larger extent than the regenerants obtained from two-month callus cultures. In some somaclones, molecular marking of the regenerants revealed specific RAPD and ISSR fragments that were absent in other somaclones or the original maize line. On the basis of six specific fragments (five RAPD and one ISSR), SCAR (Sequence Characterized Amplified Region) markers were developed. Specific polymorphism revealed with random primers was completely confirmed using five SCAR markers. Polymorphism of one SCAR marker differed from that revealed with random primers. Five SCAR fragments were inherited as simple dominant traits. One SCAR fragment displayed codominant inheritance.

Genetic Markers↗
PubMed2001

[RAPD-analysis of corn somaclones].

The genetic difference between maize line A188 and A188-derived somaclones was assessed via analysis of randomly amplified polymorphic DNA (RAPD). In total, 15 out of 17 decanucleotide primers used each allowed amplification of 2-17 fragments ranging 200-2000 bp. The RAPD patterns did not differ between individual plants of line A188, which demonstrated again its high genetic homogeneity. The difference between the initial line and the somaclones was high, ranging 64-74%. On evidence of the genetic divergence, the somaclones formed two clusters. The distribution of somaclones between these clusters was consistent with their origin.

Base Sequence↗
PubMed1982

[Photochemical activity of pea mutants with damaged photosystems].

The interrelationship between biochemical photosynthetic reactions and mutations was investigated, using five nuclear recessive lethal pea mutants with damaged photosystems II (1, 19) and I (5, 21, 22). Based on the data from photoreduction of NADP+, light-induced redox conversions of exogenous and endogenous plastocyanines in isolated chloroplasts, light-induced redox conversions of cytochrome f and absorbance changes at 520 nm in chloroplasts and leaves, the possible existence of alternative pathways of electron transfer and the pleiotropic effects of mutations, are discussed. A correlation between the structural damages of chloroplasts and the photochemical activity of mutants was found. The existence of cyclic electron transport around photosystem I involving cytochrome f and plastocyanine in mutants 1 and 19 and of an electron transfer pathway from H2O to NADP+ without plastocyanine in mutant 22 is postulated.

Chloroplasts↗
PubMed1981

[Some peculiarities of structure and protein composition of chloroplast membranes of pea mutant chlorophyll I].

The morphological properties and polypeptide composition of inner chloroplast membranes of normal and mutant pea plants (nuclear mutant "Chlorophyll I") were compared. The total fractions of chloroplast membranes from both sources were separated in a stepwise sucrose density gradient resulting in two fractions. The major fraction from mutant plants, unlike that from normal plants, does not contain thylakoids of the grana but is represented by reticular and cellulate structures, in which each cell corresponds to a thylakoid of macrograna and whose size exceeds that of normal 3 - 4-fold. In addition the inner membranes of mutant plant chloroplasts do not contain membrane polypeptides with molecular weight of 32 000 and 35 000.

Chlorophyll↗
PubMedDate not supplied

[Growth, development and genetic status of pea plants cultivated in space greenhouse "LADA"].

In the period between March 2003 and April 2005 five crops of genetically marked dwarf pea were cultivated in greenhouse LADA on the ISS Russian segment to study morphology and genetics in consecutive generations. Results of the first space experiment were analyzed and characteristics of ground plants grown from space seeds were studied. It was shown that parameters of growth, development and genetic status of pea plants that completed the ontogenetic cycle in LADA did not differ substantially from the laboratory control and that plants cultivated in space flight do not incur losses in the reproductive function and form viable seeds. Genetic analysis of plants from the first generation of space and ground seeds using the methods of RAPD-primers (10 markers) and chromosomal aberration analysis failed to reveal genetic polymorphism which means that the spaceflight factors had no effect on the genetic apparatus of the first generation of space-grown plants.

Ecosystem↗
PubMedDate not supplied

[Fasciation in pea: basic principles of morphogenesis].

A study of fasciated pea Pisum sativum L. (Fabaceae) mutant Shtambovy in comparison with the wild type (Nemchinovsky cultivar) has shown that fasciation is a result of abnormal cohesion of axial or other structures which arise in a superfluous amount due to uncontrolled meristic processes. In some cases, the organs with the same number and position as in the wild type can be fascinated. Subsequent defasciation and some features of tissue differentiation suggest that the meristem of a fasciated shoot retains a certain degree of discreteness which reflects its complex structure. The number and position of leaves in a node is a function of the diameter of the leaf primordium inhibitory zone, size of the shoot apical meristem, and number of bundles in a shoot. In the absence of the apex proliferative activity combined with the reduction of phyllomes in the upper nodes, abnormal cohesion of the second order axes, racemes, can take place. As a result, inflorescences of special type develop.

Meristem↗