PubMed Health⌕ Search

Biomedical subjects

S A Khrustalev

Publications and source records attributed to S A Khrustalev.

At least 19 recordsLinked to original sources
PubMed2003

[Problems in using methods of analyzing gene expression in studying the aging process].

Aging processes are now actively investigated on the base of analysis of changes in gene expression. Many of the standard methods (such as hierarchic clustering, method of K-means, SOM (Self-organizing maps), Gene Shaving, etc.) do not take into account the specifics of time sets. In the article the new approach of the analysis and clustering of genes based on the processing of time increments in their expressions is presented. Genes in the resulting groups behave concertedly with any chosen "pilot" gene or simply together. The methods of clustering were tested on the data of gene expressions of C. elegans in aging. In the article the specific claims of believability of the experiments are discussed.

Aging↗
PubMed2001

Effect of mesna on lethal effect and hematological toxicity of taxol and vepeside in mice.

Mesna, an SH-containing uroprotector, attenuates the lethal effect and hematological toxicity of vepeside and taxol, but did not reduce specific activities of the studied cytostatics in mice with transplanted tumors. This selective antitoxic effect of mesna towards vepeside and taxol allows to intensify the anticancer chemotherapy with these highly effective but extremely toxic cytostatics and to improve the efficiency of anticancer therapy.

Animals↗
PubMed1991

[Mouse hepatoblastoma: comparative aspects].

The structure of 12 spontaneous hepatoblastomas found in old (average age 26.5 months) male mice is described. There were considerable strain differences in their incidence: 0.5% (1/194), 0.5% (1/194) and 5% (10/198) in strain C57B1, CBA and F1 (CBA X C57B1), respectively. This proves the importance of genetic factor the role of which in the development of human hepatoblastoma is not established so far. Mouse hepatoblastoma develops almost invariably within or adjacent to liver cell tumours (adenoma or carcinoma). There was a correlation between the incidence of liver cell tumours within a given strain treated with different doses of carcinogen but such correlation was absent in mice of different strains. Histologically and ultrastructurally, mouse hepatoblastoma corresponds to the anaplastic variant of human hepatoblastoma. As distinct from human tumour, mouse hepatoblastoma does not contain alpha-fetoprotein. One tumour was transplanted to the syngeneic host and passed 30 transplant generations retaining the structure of a primary tumour with areas of osteoid tissue and foci of squamous cell metaplasia. Mouse hepatoblastoma may be induced by carcinogens. Likewise, according to the literature, risk of hepatoblastoma is higher in children whose mothers were exposed to the potential carcinogens before or during the pregnancy.

Animals↗
PubMed1990

Transplantable mouse hepatoblastoma: histologic, ultrastructural and immunohistochemical study.

Hepatoblastoma found adjacent to a liver cell adenoma in an aged (CBA x C57Bl/6)F1 male mouse was transplanted to the syngeneic host and passed through 30 generations. Histologically, tumours that grew on transplantation retained principal morphological features of the primary tumour. Transplanted tumours were negative for AFP and for antigen A6. This antigen in the normal mouse liver is found in epithelial cells lining bile ducts and ductules including the terminal Hering canals. Some of the Hering cells were consistently A6 negative under normal conditions, and the suggestion is made that these A6 negative cells might be the cells of hepatoblastoma origin.

Adenoma↗
PubMed1989

[Genetic and oncologic characteristics of CBA/CaLacSto mice].

400 male and female CBA/CaLacSto mice were analyzed from the age of two months to their death. The spontaneous malignancy incidence of frequency in this line amounted to 76.3% with prevailing hepatomas (65%), neoplasms of lung, fat tissue. Homozygosity of CBA/CaLacSto mice was revealed in all the gene under test.

Alleles↗
PubMed1988

[Spontaneous hepatomas in hybrid mice (CBA x C57Bl/6)F1].

The spontaneous hepatomas of male mice (CBA X C57B1/6)F1 were studied using complex cytologic, histologic, immunochemical, and cytochemical methods. The AFP was detected in trace amount in the serum of tumour-bearing mice and in single cells of hepatomas. As a rule the hepatocarcinoma cells have the polymodal DNA distribution (2C-8C) with major peak of 4 C (in 18 out of 26 cases). More rarely the tumours with predominant diploid and octoploid cells were observed.

Animals↗
PubMed1987

[Ploidy level and alpha-fetoprotein production in spontaneous murine hematomas in relation to the degree of histological differentiation].

Comparative morphological, cytospectrophotometric and immunochemical studies were carried out on 26 hepatocarcinomas of various malignancy excised from 21 F1 (CBA X C57B16) mice. It has been shown that in morphologically similar hepatomas the ploidy level and alpha-fetoprotein production intensity may vary markedly. Highly differentiated carcinomas usually of smaller sizes and homogeneous morphology have the modal class nuclei with tetraploid (6 cases) or octaploid (3 cases) DNA content. In moderately- and low-differentiated carcinomas the diploid class of nuclei significantly increased (in 4 out of 6 tumours). Large tumours morphologically heterogeneous according to the differentiation level had polymodal DNA distributions with predominating tetraploid nuclei (II cases).

Animals↗
PubMed1986

[Effect of the carcinogenic tyrosine metabolite p-hydroxyphenyllactic acid on the ascorbic acid concentration in the organs and blood of mice].

The effect of cancerogenic tyrosine metabolite, p-hydroxyphenyllactic acid, on the concentration of ascorbic acid in the organs and blood of mice has been studied. p-Hydroxyphenyllactic acid was demonstrated to decrease considerably ascorbic acid concentration in the liver, adrenal glands and blood of mice. The above phenomenon and the previous data on tyrosine aminotransferase induction by p-hydroxyphenyllactic acid suggest the existence of two interdependent mechanisms of cancerogenic tyrosine metabolite (p-hydroxyphenyllactic acid) accumulation.

Animals↗
PubMed1985

[Oncological characteristics of C57Bl/6JY mice].

The oncological characteristic is presented for C57BL/6JY mice widely used in experiments. The mice over eighteen month had different spontaneous neoplasms among which hemoblastoses prevailed. The influence of sex, age and vaccination on the rate of spontaneous neoplasms in these mice is analyzed.

Adenoma↗
PubMed1984

[Morphological and cytochemical characteristics of spontaneous hemoblastoses in AKR mice].

Spontaneous hemoblastoses of AKR mice are widely used in experimental oncology and hematology. To study their morphological characteristics, 500 AKR mice were used. Of these, 400 animals were followed up throughout their lives. Ten animals were sacrificed monthly out of the group of 100 rats. All the animals which died or were sacrificed were autopsied and subjected to cytological and histological studies. Besides, 36 animals with hemoblastoses were examined cytochemically for the activity of acid and alkaline phosphatases, peroxidase and nonspecific esterase and glycogen. Analysis of the data obtained suggests that hemoblastoses of AKR mice are the generalized forms of lymphosarcoma, characterized by a significant degree of cytochemical and cytological heterogeneity.

Acid Phosphatase↗
PubMed1984

[Oncological characteristics of AKR/JY mice].

The oncological characteristic of AKR/J mice widely used in experimental investigations is presented. The incidence of spontaneous hemoblastoses in this subline is sufficiently high during the last ten years, that allows to use this subline as an experimental model of hemoblastoses. The analysis of the influence of sex factors, age and vaccination on the rate of spontaneous tumours of the lymphoid tissue is given.

Animals↗
PubMed1979

[Morphological analysis of spontaneous hemoblastoses in dogs and cats].

Information of the frequency and morphological characteristics of spontaneous hemoblastoses in dogs and cats observed in 1966-1978 are presented. Hemoblastoses were diagnosed in 90 dogs and 12 cats. Lymphoproliferative diseases were most frequent: lymphosarcomas (80 cases) and lymphocytic leukemias (11 cases). Ten dogs had mastocytomas and one--myeloid leukemia. The cell composition of the organs and tissues involved permitted to distinguish 3 cellular variants of lymphosarcoma: poorly differentiated, lymphoblastic, and prolymphocytic; in both species the poorly differentiated type of lymphosarcoma was predominant (47 cases). It is recommended to use spontaneous hemoblastoses of dogs and cats as an experimental model for investigations of the pathogenesis and therapy of human leukemias.

Animals↗