The production of bacteriolytic enzymes by Staphylococcus aureus in batch and continuous culture.
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Bacteriolysis↗
Biomedical subjects
S Arvidsson
Publications and source records attributed to S Arvidsson.
Use of formalin fixed, paraffin embedded tumours for estimation of cellular DNA content and S-phase fraction by static cytofluorometry.
Sections with a thickness of 50 micron from 34 formalin fixed, and paraffin embedded tumours were deparaffinized and hydrated and the cells were disaggregated with pepsin. The cells were stained with Hoechst 33258 and a rapid method for static fluorometry was used. DNA ploidy and the fraction of cells in S-phase were estimated and compared with results obtained with freshly prepared cells. The coefficients of variation for the tumour stemlines for both the fresh and the embedded material were approximately 6 per cent. There was a good correlation between DNA ploidy determined from fresh and embedded material, respectively; there was also a close correlation between the fractions of S-phase cells estimated from fresh and embedded tumours. It was also possible to cut out small histologically defined lesions, such as carcinoma in situ of the breast from thick paraffin sections and obtain DNA histograms with high resolution.
Bisbenzimidazole↗
Cerebral perfusion in dogs during pulsatile and non pulsatile extracorporeal circulation.
Eight dogs were cannulated for left heart bypass. Conventional roller pumps were used for bypass with one of the pump console modified to deliver pulsatile bypass. The experiments were performed at normothermia. Each dog was used as its own control. For measurement of cerebral blood flow (CBF) Xe133 was injected into vertebral artery and the gamma activity elimination over the skull was registered. In addition blood samples were withdrawn from the axillary artery and the sagittal sinus for measurement of blood gases. The CBF to the gray matter of the brain was significantly higher during the periods with pulsatile perfusion (P less than 0.01). This was probably due to decreased total cerebral vascular resistance (P less than 0.001). Mean aortic pressure also decreased when the mode of perfusion was changed from non pulsatile to pulsatile (P less than 0.05), indicating a general decrease of the peripheral vascular resistance. A good correlation was noted between the arteriovenous oxygen saturation difference (AV-O2 diff) across the brain and the CBF (R = -0.77, P less than 0.01).
Animals↗