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Biomedical subjects

S C Parija

Publications and source records attributed to S C Parija.

At least 73 records · Page 4Linked to original sources
PubMed1997

Detection of hydatid antigen in urine by countercurrent immunoelectrophoresis.

Hydatid antigen was demonstrated for the first time in the urine of patients with hydatid disease by countercurrent immunoelectrophoresis (CIEP). The antigen was detected in the concentrated urine of 7 of 16 (43.75% positive) patients with surgically confirmed hydatid disease, 4 of 10 (40% positive) patients with ultrasound-proven hydatid disease (daughter cysts or prominent septation and hydatid sands demonstrated by ultrasound), and 8 of 14 (57.14% positive) patients with clinically diagnosed (presumptive) hydatid disease. No antigen was detected in the concentrated urine from 24 patients with parasitic diseases other than hydatid disease. However, antigen was detected in 2 (8% false positive) of 25 concentrated urine samples collected from healthy control subjects (blood donors and students). These result suggest that the detection of hydatid antigen in the urine by CIEP is a simple, rapid, and noninvasive method of diagnosis of hydatid disease.

Animals↗
PubMed1995

Evaluation of lacto-phenol cotton blue for wet mount preparation of feces.

The use of lacto-phenol cotton blue (LPCB) stain for wet mount preparation of stools to demonstrate intestinal parasites by routine microscopy was evaluated in this study. LPCB-stained trophozoites and cysts and helminthic ova could easily be detected and identified in LPCB wet mounts of stools. The stain is recommended for routine use in the wet mount preparation of stools in a parasitology laboratory.

Ancylostomatoidea↗
PubMed1995

Stool culture as a diagnostic aid in the detection of Entamoeba histolytica in the faecal specimens.

"The use of stool culture in Boeck & Drbohlav's biphasic amoebic medium as a routine diagnostic aid in the detection of Entamoeba histolytica in the faeces, is evaluated in the present study. A total of 3803 faecal specimens were examined for the presence of E.histolytica by direct smear, formalin ether concentration and culture during a study period of 1982-1990. A total of 259 stool specimens were positive for the parasite by any or all of these methods 42 (16.21%) stool specimen not cultured in Boeck & drbohlav's medium were possible by direct smear and concentration methods. 99 (38.22%) stool specimens were positive by all of these methods (direct smear, concentration and culture). The culture detected E. histolytica in additional 62 (23.93%) stool specimens which were negative by both the direct smear and concentration methods. Results of this study recommends the use of stool culture as a routine diagnostic aid in the laboratory, for the detection E. histolytica in the faeces".

Animals↗
PubMed1994

Effects of adenosine on contractility and 45Ca-uptake in rat urinary bladder.

Effects of adenosine on K+ and ACh-stimulated contractility and 45Ca uptake were studied in the rat urinary bladder smooth muscle and were compared with those of nifedipine. Both adenosine (10(-5) M) and nifedipine (10(-7) M/10(-8) M) significantly inhibited the contractions elicited by K+ (10(-2)-32 x 10(-2) M), Ca2+ (10(-4)-3 x 10(-2 M) in K(+)-depolarized preparations and ACh (10(-9) M-3 x 10(-3) M). Further, adenosine (10(-5) M) significantly (P < 0.05) inhibited K+ (10(-1) M)-stimulated 45Ca-uptake in the bladder strips. However, it had little effect on inward 45Ca movement resulting from ACh (10(-4) M)-induced stimulation. On the other hand, nifedipine (10(-7) M) significantly (P < 0.001) reduced both K+ and ACh-induced 45Ca-uptake in this tissue. It is concluded that the calcium channel blocking action of adenosine is limited to Ca2+ uptake through voltage operated calcium channels, while receptor operated calcium channels activated by muscarinic receptor stimulation appear to be insensitive to the purine.

Adenosine↗
PubMed1993

Co-agglutination (Co-A) test for circulating antigen in hydatid disease.

A co-agglutination (Co-A) test with Staphylococcus aureus (Cowan's strain I) bearing protein A coated with specific hydatid antibodies, was used to demonstrate circulating hydatid antigen in serum for the diagnosis of hydatid disease. The test had a sensitivity of 95% and specificity of 89% in detecting hydatid antigen in serum. A false positive reaction was observed with 18.5% of control sera from patients with various parasitic diseases. However, these all gave negative results when tested after further dilution. The test is reliable and rapid; the result could be obtained within 30-45 min of receipt of a serum sample. Since the test is simple and the reagents are inexpensive and easily available, it can be used in less well equipped laboratories in developing countries.

Agglutination Tests↗
PubMed1993

Effect of nifedipine and dipyridamole on K+ and NA stimulated 45Ca-uptake into rabbit aortic strips.

The effect of dipyridamole (DPM), a purine nucleoside uptake inhibitor, on the K+ and noradrenaline (NA)-stimulated 45Ca-uptake into Rabbit aortic strips was studied and compared with that of nifedipine (NFD). DPM (10(-6) M) significantly (P < 0.02) inhibited 45Ca-uptake in aortic strips stimulated with K+ (10(-1) M). However, it had less significant effect on NA (10(-4) M)-stimulated 45Ca-uptake. The percentage inhibition was 80 and 57 in K+ and NA-stimulated 45Ca-uptake, respectively. Similarly, NFD (10(-7) M) caused significant (P < 0.01, P < 0.05) decrease in K+ (0.060 +/- 0.009) and NA (0.082 + 0.01, mM)-stimulated 45Ca-uptake. The percentage inhibition by NFD was 98 and 64 in K+ and NA-stimulated 45Ca-uptake, respectively. The results suggest that DPM inhibits Ca2+ influx occurring through Voltage Operated Calcium Channels (VOCCs) but it has little effect on Receptor Operated Calcium Channels (ROCCs).

Animals↗
PubMed1992

Prevalence of hookworm species in Pondicherry, India.

A study was carried out to find out the hookworm species prevalent in Pondicherry, India, based on the identification of filariform larvae recovered from coproculture of hookworm positive stools. A total of 184 hookworm positive stools were cultured using the Harada-Mori coproculture method. The results of the study for the first time showed both hookworm species, Ancylostoma duodenale (60 cases) and Necator americanus (49 cases) to be present in Pondicherry. The former species was relatively more common than the latter. Three cases were infected with both species of hookworm.

Feces↗
PubMed1991

Adenosine- and alpha,beta-methylene ATP-induced differential inhibition of cholinergic and non-cholinergic neurogenic responses in rat urinary bladder.

1. The effects of adenosine and alpha,beta-methylene adenosine triphosphate (alpha,beta-Me ATP) on single pulse-induced neurogenic responses and contractions caused by exogenously applied acetylcholine (ACh) and adenosine triphosphate (ATP) were examined in rat urinary bladder. 2. Application of single pulse stimulation (1 ms; 80 V) evoked a biphasic contractile response (abolished by tetrodotoxin, 0.5 x 10(-7) M) consisting of a fast (time to peak: 1.02 +/- 0.07 s) and a slow component (time to peak: 4.92 +/- 1.6 s). The selective inhibition of the slow component by atropine (3 x 10(-6) M) suggests the participation of both cholinergic and non-cholinergic neurotransmitters. 3. alpha,beta-Me ATP (5 x 10(-6) M) abolished ATP (10(-4) M)-induced contractions without altering those to ACh (10(-6) M). Further, the selective inhibition of the fast component of the neurogenic response by alpha,beta-Me ATP is suggestive of the contribution of endogenous ATP to the non-cholinergic component. 4. Adenosine (10(-8) M to 10(-4) M) caused dose-related differential inhibition of the fast (IC50, 1.04 +/- 0.25 x 10(-5) M) and slow (IC50, 2.18 +/- 0.69 x 10(-6) M) components, thereby further supporting two modes of neurotransmission in bladder. 5. Theophylline (10(-4) M) antagonized the inhibitory effects of adenosine on the non-cholinergic component, thereby implicating the participation of P1-purinoceptors in neuromodulation. In contrast, theophylline at this concentration enhanced the adenosine-induced inhibition of the cholinergic component. component. 6. The magnitude of ATP (10-4M)- and ACh (10-8M)-induced contractions were almost identical to those of the fast and slow components of the neurogenic response, respectively. Comparable reduction of ATP (30.2 + 3.4%) and ACh (100%) contractions to those of fast (44.2 + 6.5%) and slow (88.2 + 5.5%) components suggests the involvement of a postjunctional mechanism in adenosine-induced differential inhibition of neurogenic responses. 7. The lack of effect of erythro-6-amino-9-(2-hydroxy-3-nonyl) adenosine hydrochloride (10-6M) and dipyridamole (10-6M) suggests that endogenous adenosine plays little part in modulation of single pulseinduced neurogenic response. 8. The results of the present study suggest that fast and slow components of neurogenic response are mediated through ATP and ACh, respectively, possibly co-released from the same neurone in the rat bladder.

Acetylcholine↗
PubMed1991

Inhibition of contractions and 45Ca influx by adenosine in rabbit aorta.

The effects of adenosine on contractility and 45Ca-uptake in rabbit aorta have been examined and compared with those of nifedipine. Both adenosine (10(-5) M) and nifedipine (10(-8) M) inhibited contractions caused by CaCl2 (0.1-10 mM) in K(+)-depolarized preparations. Nifedipine (10(-7) M) significantly inhibited the contractions of noradrenaline (NA) at all doses (10(-8)-10(-4) M), while adenosine inhibited the contractions induced only by lower doses of NA (10(-8) and 10(-7) M) without affecting those at higher doses (greater than 10(-7) M). Adenosine (10(-8) M) and nifedipine (10(-7) M) inhibited K(+)-stimulated 45Ca-uptake by aortic strips by 75 and 100%, respectively. Similarity, NA (10(-4) M)-stimulated 45Ca influx was inhibited by about 70% by both these agents. The results suggest that adenosine inhibits inward Ca2+ movement involving both voltage-operated and receptor-operated calcium channels in rabbit aortic smooth muscle.

Adenosine↗
PubMed1991

Abdominal hydatidosis in Pondicherry, India.

A study during the period 1980-1987 revealed 71 surgically confirmed cases of abdominal hydatid disease. The majority of the patients were in 3rd, 4th and 5th decades (64.5%) with approximately equal incidence in both males and females. The commonest site of lesion was liver (56.4%), followed by peritoneal cavity (16.9%) and spleen (10.2%). Indirect hemagglutination serology test was found to be more sensitive (95.2%) than the traditional Casoni skin test in the diagnosis of abdominal hydatid disease. Ultrasonography was also found to be a useful procedure in the diagnosis of the condition. This study shows that there is a significant rise in the number of patients suffering from abdominal hydatid disease in this part of India.

Adolescent↗
PubMed1991

Recent trends in the serodiagnosis of hydatid disease.

Hydatid disease caused by Echinococcus granulosus is a zoonotic infection of cosmopolitan distribution. As the clinical manifestations of hydatid disease in man are variable, the diagnosis of the condition presents complex problems for clinicians. Since the parasitic diagnosis of the disease is difficult, the specific diagnosis of the condition relies heavily on immunodiagnostic tests. The recent approach to the diagnosis of hydatid disease in man is primarily based on: (1) a combination of two or three more serological tests to diagnose the condition, as a single test fails to detect all the cases, (2) detection of circulating hydatid antigen (CAg) in the serum by enzyme-linked immunosorbent assay (ELISA) and other assays, as the antigen detection system is useful in monitoring post-surgical and chemotherapeutic evaluation of the cases as well as in the prognosis of the condition and (3) demonstration of E. granulosus antigen in the cystic fluid to establish the etiology of the hydatid cyst. Hydatid disease is essentially a disease of poor people residing in rural areas, hence there is need for a simple, economic diagnostic immunoassay for use at the field level or in a rural health center with inadequate facilities. Counter-current-immunoelectrophoresis (CIEF) and bacterial co-agglutination (Co-A), have been standardized and evaluated in this laboratory for the first time for detection of CAg in cases of hydatid disease at the field level and rural health center.

Animals↗
PubMed1991

Protein A-antibody mediated hemagglutination assay for serodiagnosis of amebic liver abscess.

A successful modification of the indirect hemagglutination test to demonstrate antibodies for serodiagnosis of amebic liver abscess has been described in the present study. In the modified test, the protein A-IMA, Staphylococcus aureus (Cowan's strain I) bearing protein A (SAPA) cells were used to enhance hemagglutination of sensitized red cells. Use of SAPA cells markedly enhanced sensitivity of the test and greatly increased the titers obtained with most of the sera. At a diagnostic antibody titer of 1:128 and above, the protein A-IHA could detect 72 (100%) of amebic liver abscess (ALA) cases. Amongst the controls, no false positive reaction was observed in non-amebic liver disease controls. However 1(2%) of sera demonstrated false positive reactions from healthy controls. The protein A-IHA was highly sensitive when compared with that of the indirect hemagglutination (IHA) for serodiagnosis of amebic liver abscess. The novel immunoassay is simple, inexpensive and requires little technical skill. It has the potential for wide application in serodiagnosis of amebic liver abscess.

Animals↗
PubMed1989

Rapid indirect haemagglutination (rapid-IHA) using sensitized chick cells for serodiagnosis of amoebiasis at primary health centre level.

A successful modification of the indirect haemagglutination (IHA) test as a simple and rapid diagnostic procedure for serodiagnosis of amoebiasis at a primary health centre (PHC) level laboratory has been described in the present study. In this modified IHA test, the chick red blood cells (RBC), stabilized by the double aldehyde method, could be sensitized with amoebic antigen and then stored at 4 degrees C for up to 45 days for their subsequent use, as ready-made reagent directly in the IHA, without loss of sensitivity. The chick red cells settled quickly and their haemagglutination pattern could be determined within 30 to 45 min of incubation at room temperature with test sera. The IHA using double-aldehyde stabilized (DAS) chick cells, showed equal sensitivity and specificity with that of IHA using human O cells in diagnosing cases with different manifestations of amoebiasis. With the use of stored sensitized DAS chick cells directly in the IHA, the test could be performed and results obtained within 60 to 90 min of receipt of the sera to be tested. This makes IHA a simple and rapid procedure for its adaptation at a PHC level laboratory for the serodiagnosis of amoebiasis.

Amebiasis↗
PubMed1989

A study on laboratory diagnosis of liver abscess in Pondicherry.

A study was carried out to evaluate the role of laboratory investigations for diagnosis of liver abscess from the year 1976 to 1981. Bacteriological, parasitological, serological, haematological, histopathological studies and liver function tests were carried out in 240 confirmed cases of liver abscess during the study period. Out of 219 cases, pus culture for pyogenic bacteria was positive in 68 cases (including 27 cases of secondary bacterial infection of amoebic liver abscess) and sterile in 151 cases. Ent histolytica was demonstrated in pus of 24 cases. The diagnostic titre of 1:128 and above was detected in 137 sera by isohaemagglutination test. The liver function tests were of limited value. Albumin globulin ratio was altered in 91.25% cases and there was moderate increase in the levels of alkaline phosphatase. Histopathological studies demonstrated Ent histolitica in 2 cases only. Changes characteristic of pyogenic liver abscess were observed in 6 cases. Rest showed no specific changes.

Humans↗