PubMed Health⌕ Search

Biomedical subjects

S Carey

Publications and source records attributed to S Carey.

71 records · Page 4Linked to original sources
PubMed1978

Reexpression of HPRT activity following cell fusion with polyethylene glycol.

Polyethylene glycol-1000 (PEG-1000) induced fusion of HPRT (E.C. 2.4.2.8) deficient Chinese hamster cells with alpha-galactosidase A (E.C. 2.3.1.22) deficient cells from a patient with Fabry's disease yielded hybrids which contained both human and hamster HPRT, G6PD (E.C. 1.1.1.49), and APRT (E.C. 2.4.2.7) and Chinese hamster alpha-galactosidase B. Thus PEG-1000 mediated somatic cell fusion led to reexpression of Chinese hamster HPRT. It did not restore the expression of human alpha-galactosidase. Since PEG-1000 treatment of HPRT- Chinese hamster cells in the absence of human cells yielded no HPRT+ cells, it is concluded that the element responsible for the restoration of rodent HPRT was contributed by the human cells and not by the agent employed to promote fusion.

Adenine Phosphoribosyltransferase↗
PubMed1978

Upright and inverted faces: the right hemisphere knows the difference.

The existence of a right hemisphere capacity, specific to upright faces was investigated. Upright and inverted faces, equally complex as patterns, were presented under lateralized tachistoscopic conditions to two groups of normal adult subjects. A significant orientation by visual field advantage was found. While there was a highly significant left visual field advantage for upright faces, the visual field difference for inverted faces failed to reach significane. This pattern of results supports the hypothesis that the right hemisphere is specialized for the perception of faces in particular, in addition to its specialization for the perception of visuospatial patterns in general.

Adult↗
PubMed1977

From piecemeal to configurational representation of faces.

Unlike older children and adults, children of less than about 10 years of age remember photographs of faces presented upside down almost as well as those shown upright and are easily fooled by simple disguises. The development at age 10 of the ability to encode orientation-specific configurational aspects of a face may reflect completion of certain maturational changes in the right cerebral hemisphere.

Aging↗
PubMed1974

Determination of plaque inhibitory activity of adenine arabinoside (9-beta-D-arabinofuranosyladenine) for herpesviruses using an adenosine deaminase inhibitor.

The in vitro susceptibility of type 1 and type 2 strains of Herpesvirus hominis to 9-beta-d-arabinofuranosyladenine (adenine arabinoside, ara-A) was measured in a system where deamination was inhibited. Under these conditions, it was possible to measure the activity of low concentrations of ara-A. It was determined that plaque inhibitory concentration for type 1 viruses was less than 3 mug/ml for all strains tested. The plaque inhibitory concentration for 7 of 10 type 2 strains was also less than 3 mug/ml. The method used identified and controlled the interaction between antiviral agent (ara-A) and the indicator system, human skin fibroblastic cells. Otherwise, metabolism of ara-A resulted in rapid enzymatic degradation and loss of antiviral activity.

Adenosine Deaminase Inhibitors↗
PubMed1974

Effect of adenosine deaminase upon the antiviral activity in vitro of adenine arabinoside for vaccinia virus.

This study determined that the effect of 9-beta-d-arabinofuranosyl-adenine (adenine arabinoside, Ara-A) upon vaccinia virus plaque development in the stable monkey kidney line, LLC-MK(2), was increased approximately 40-fold when an inhibitor of adenosine deaminase (ADA) was added to the tissue culture media along with infective inocula. The concentration of Ara-A required to completely suppress plaque development (total plaque inhibitory concentration(100); TPIC(100)) was greater than 10 mug/ml. However, when ADA activity was inhibited, the TPIC(100) was 0.5 mug/ml or less. Chromatographic assay of arabinosylpurines in the media provided evidence that adenine arabinoside was rapidly deaminated to 9-beta-d-arabinofuranosylhypoxanthine by the cellular monolayers, in the absence of animal serum, and that the rate of deamination, at 5 mug/ml, by the cells was equal to the rate of diffusion of Ara-A across the cellular membrane. The half-life of Ara-A in the media, starting with 5 mug/ml, was 2 to 3 h and shorter at lower concentrations. The study demonstrates the profound effect that an indicator system, acting as an intact biological unit, can have upon a potential antiviral compound.

Adenosine Deaminase↗
PubMedDate not supplied

Information technology and computer-based decision support in diabetic management.

This paper describes the application of computer-based techniques within an intelligent, knowledge-based framework to the management of diabetes. The objectives are to structure data collection and storage so that the relevant patient-specific data are collected and made accessible as needed, and to provide clinical decision support on either a day-by-day or longer timescale as appropriate; these objectives relating to both hospital clinic and general practice. For longer-term management, a prototype rule set (greater than 500 rules) has been developed (coded in Sigma PROLOG), validated and tested on patient data. The data collection programs (written in SCULPTOR) to feed the ruleset have been tested in the hospital clinic and compared with the resident data collection system for usability, and impact on the running of the clinic. Links between the data collection programs and the ruleset program have been written and tested. The computer system will also incorporate a module, combining knowledge-based advisory system and glucose/insulin model as patient simulator, that can be tested as a potential decision aid for adjusting insulin dosage on a daily basis.

Data Collection↗
PubMedDate not supplied

Predicting the outcomes of physical events: two-year-olds fail to reveal knowledge of solidity and support.

Two-year-olds' (N = 153) knowledge of solidity was tested in four search tasks adapted from infant looking-time experiments. In Experiment 1, 2-year-olds failed to search in the correct location for a falling ball after a hidden shelf that blocked its trajectory had been inserted in the apparatus. Experiment 2 extended this finding by showing that 2-year-olds failed to take into account the effects of either removing or inserting a shelf in their search for a toy dropped behind a screen. Experiment 3 examined sensitivity to the constraint provided by a solid barrier on horizontal motion. In all three experiments, 2-year-old children searched initially at the location where they saw the object during familiarization. Experiment 4, using multiple test trials but no familiarization to a pretest location, also showed that 2-year-olds failed to take the presence or absence of a barrier into account when planning where to search for a toy they had seen dropped behind a screen. In all of these studies, 2-year-olds showed no evidence of representing solidity and support constraints on the trajectories of falling objects. Experiments 1 and 3 also included 2 1/2-year-olds (N = 31), who succeeded on these search tasks. The implications of the poor performance of 2-year-olds, in the face of success by very young infants on looking-time measures of sensitivity to similar constraints on object motion, are discussed.

Attention↗
PubMedDate not supplied

Nursing observations of central venous catheters. The effect on patient outcome.

The purpose of this descriptive study was to obtain information concerning rates of intravascular infection in patients with central lines and to identify the variables related to nursing care of these lines that may influence the development of systemic infection. The study was conducted at a large southern teaching hospital during a 6-week period. The sample for this study consisted of adult inpatients who underwent central-line insertions. The results suggested that careful nursing observations, combined with early recognition and intervention, can significantly influence a positive patient outcome.

Adult↗