PubMed Health⌕ Search

Biomedical subjects

S Eda

Publications and source records attributed to S Eda.

At least 55 records · Page 3Linked to original sources
PubMed1996

Histological suture changes following retraction of the maxillary anterior bone segment after corticotomy.

By cephalometric and dental cast analyses, the effects of corticotomy in combination with 45-50 days retraction of the maxillary anterior segment were evaluated in five female Japanese monkeys (Macaca fuscata). In addition, microscopic changes of the related sutures were examined. According to the analyses of the dental casts, the retraction of the maxillary anterior segment in the experimental group was increased when compared with that of the control group. There was no change in occlusal molar relationship. The cephalometric analysis revealed that the "Point A" and "Metal Implant" on the mid-sagittal site between the upper central incisors in the experimental group showed more pronounced retraction than that in two untreated control animals. These results were histologically evaluated by changes of the suture structure: irregularity of functional arrangement of component cells and fibers, an increase in number of cells, resorption and formation of bone, and an enlargement of the suture width. Resorption of tooth roots and alveolar bone, and irregularity of the periodontal ligament were observed. In general, these histological changes appeared more extensive in the control specimens compared with the experimental specimens. The osseous histological changes were mainly observed in the compact bone area, while in the spongy bone area of both control and experimental specimens only minimal changes occurred. These results demonstrated the efficacy of combining a corticotomy procedure with retraction of the maxillary anterior bone segment by an orthodontic force.

Animals↗
PubMed1996

Binding characteristics of human lactoferrin to the human monocytic leukemia cell line THP-1 differentiated into macrophages.

The characteristics of the binding of human lactoferrin (LF) to the cells of a human monocytic leukemia cell line, THP-1, were investigated. 125I-Labeled LF (125I-LF) bound to THP-1 cells, and the binding increased markedly as the cells matured into macrophages (THP-1 macrophages) by stimulation with phorbol 12-myristate 13-acetate. Scatchard analysis of the binding of 125I-LF to THP-1 macrophages indicated that high and low affinity receptor sites (Kd = 0.57 x 10(-6) and 3.7 x 10(-6) M, respectively) are present on the cells. The number of these high and low affinity receptor sites were 2.4 x 10(6), and 2.5 x 10(6) per cell, respectively. Removal of iron from 125I-LF did not affect its binding to THP-1 macrophages, indicating that the binding is not dependent on Fe(III) ion. The binding of the labeled LF to THP-1 macrophages was markedly decreased following acetylation, suggesting that the amino residues of the polypeptide portion of LF play a major role in the binding. The binding of labeled LF was partially inhibited by the isolated whole oligosaccharides of LF, and by the isolated whole oligosaccharides of band 3 glycoprotein of human erythrocyte membrane which contain poly-N-acetyllactosaminyl saccharide chains, like the LF oligosaccharides. Their inhibitory activity did not depend on the terminal sialyl residues of the saccharide chains. Lacto-N-fucopentaose III and lacto-N-neotetraose, an analogous structure being present in the poly-N-acetyllactosaminyl chains of LF, also artially inhibited the binding of 125I-LF to the THP-1 macrophages. When poly-N-acetyllactosaminyl saccharide chains of 125I-LF were cleaved by endo beta-galactosidase, the binding of 125I-LF was partially reduced. These results suggest that binding of LF to THP-1 macrophages is primarily mediated by its protein component, but a short oligosaccharide structure, possibly Gal beta 1-4GlcNAc beta 1-3Gal, which is contained in the nonreducing terminal region of poly-N-acetyllactosaminyl saccharide chains of LF and band 3, and in lacto-N-fucopentaose III and lacto-N-neotetraose is also recognized by THP-1 macrophages, and this recognition partly contributes to the binding of LF to cells.

Carbohydrate Sequence↗
PubMed1996

Recognition of poly-N-acetyllactosaminyl saccharide chains on iron-oxidized erythrocytes by human monocytic leukemia cell line THP-1 differentiated into macrophages.

The cells of the human monocytic leukemia cell line THP-1 differentiated into macrophages bound to human erythrocytes oxidized with adenosine 5'-diphosphate (ADP)-Fe3+ chelate (ADP/Fe3+) in the absence of serum. The binding was prevented when the cells were treated with ADP/Fe3+ in the presence of antioxidants, indicating that oxidation of the cells is responsible for the increased susceptibility to the THP-1 cell binding. Galactose, fucose, mannose and mannan partially inhibited the binding. Glycoproteins containing poly-N-acetyllactosaminyl saccharide chains such as band 3 glycoprotein isolated from human erythrocyte membrane and lactoferrin, and their oligosaccharides, strongly inhibited the binding. On the other hand, glycoproteins with non-poly-N-acetyllactosaminyl saccharide chains such as glycophorin A isolated from the erythrocyte membrane, fetuin and alpha 1-acid glycoprotein, little or partially inhibited the binding. The inhibitory activity of band 3 oligosaccharides and lactoferrin oligosaccharides was little affected by treatment with endo-beta-galactosidase, which specifically cleaves poly-N-acetyllactosamine to shorter oligosaccharides. Removal of the nonreducing terminal region of the saccharide chains of band 3 on the erythrocyte surface by treatment of the cells with endo-beta-galactosidase resulted in a decrease in the susceptibility of the cells to the THP-1 cell binding. These results suggest that THP-1 cells which have been differentiated into macrophages bind the oxidized erythrocytes primarily through the recognition of poly-N-acetyllactosaminyl saccharide chains of band 3, and the site of the recognition exists in the nonreducing terminal region of the saccharide chains. Clustering of band 3 molecules is proposed as a possible alteration of oxidized erythrocyte membrane which promotes the interaction of the saccharide receptor on THP-1 cells with the saccharide chains of band 3 on erythrocytes.

Adenosine Diphosphate↗
PubMed1995

[Clinical evaluation of latex photometric immunoassay for serum CA19-9].

Latex photometric immunoassay for CA19-9 was clinically evaluated. Reference interval of the assay was determined as 37U/ml based on the data of 350 healthy donors. CA19-9 values were higher in females than in males (p < 0.01) and distribution of CA19-9 values varied significantly depending on the Lewis blood types of the subjects; namely, subjects with Lea+b- gave highest CA19-9 values followed by those with Lea-b+ and then those with Lea-b- (p < 0.001). In the patients with various malignant diseases, positive rates were high (> 60%) in the patients with pancreatic, hepatic, and colon cancer. Meanwhile, false positive rates were around 25-50% in the patients with pancreatitis, cholelithiasis, benign hepatic disorders and benign lung diseases. When cut-off value was set at 100U/ml, false positive rates decreased drastically, while true positive rates remained slightly decreased. These findings indicate that, in this latex assay, CA19-9 values of 100U/ml or higher should be regarded as high risk factor of malignancy and values in the range of > or = 37U/ml and < 100U/ml might be considered as warning signal of various benign and malignant diseases.

Biomarkers, Tumor↗
PubMed1995

[Septic lung caused by methicillin-resistant Staphylococcus epidermidis].

A 55-year-old man was admitted to our department one month after resection of rectal carcinoma, with complaints of fever and general malaise. Shock developed rapidly after admission. The chest X-ray film and computed tomography showed diffuse small nodular shadows and bilateral pleural effusion. Septic lung caused by Methicillin-resistant Staphylococcus epidermidis (MRSE) was diagnosed from the results of a bacteriological study. This bacteria is a Coagulase Negative Staphylococcus (CNS). Chemotherapy with Minocycline and Cefotiam was effective. Characteristic radiologic features of this case may be related to the early stage of adult respiratory distress syndrome (ARDS) and septic pulmonary microembolism.

Anti-Bacterial Agents↗
PubMed1994

Two cases of mandibular radicular cysts with ciliated epithelium: an electron microscopic study.

We studied two cases of mandibular radicular cysts lined in part with ciliated epithelium by examining them histopathologically and ultrastructurally. In addition, we examined the incidence of cilia in 205 radicular cysts in the biopsy materials collection in our Department. No other mandibular cysts having cilia in the lining were found, and so these were very rare. Regarding the histogenesis of the cilia, we consider that the development of cilia in mandibular radicular cysts may be due to true metaplasia rather than to adaptation which is a functional requisite of the lining epithelium for the unique cystic environment.

Adult↗
PubMed1993

Squalane as a possible carrier of bone morphogenetic protein.

Gelatin capsules containing squalane partially purified bone morphogenetic protein (BMP) complex were placed on the perimuscular membrane of rats. Two kinds of control, gelatin capsules containing only BMP and those bearing squalane only, were used. The embedded areas were histopathologically examined at 3 and 6 wk after the operation. The observations revealed that the squalane/BMP complex elicited wide heterotopic bone formation with bone marrow tissue, suggesting that squalane is a possible carrier of BMP for clinical applications.

Animals↗
PubMed1993

Hyperkeratosis and atypical proliferation appearing in the lining epithelium of a radicular cyst. Report of a case.

It is widely accepted that radicular cysts (apical periodontal cysts) are commonly lined with stratified squamous epithelium without keratin formation. However, we identified a case of maxillary radicular cyst with remarkable keratinization and atypical proliferation of the lining epithelium among the 207 radicular cyst cases seen at our department. Histopathological and clinical findings of these cysts were reviewed.

Cell Division↗
PubMed1992

Experimental study on osteoconductive properties of a chitosan-bonded hydroxyapatite self-hardening paste.

A developmental bone substitute, composed of a chitosan-bonded hydroxyapatite paste and having various possible applications in medical and dental treatments, was evaluated with regard to its osteoconductive properties. Radiographic examination revealed that a bone-like irregular radiopacity appeared in the region of the embedded paste. This was judged histopathologically as the formation of bone tissue with chondral tissue. These data suggest that the paste has osteoconductive properties, and may, therefore, prove clinically useful as a bioactive bone substitute.

Animals↗
PubMed1992

Pulpal and radicular changes following maxillary subapical corticotomy.

Pulpal and radicular changes after subapical corticotomy in combination with posterior transfer of the maxilla were studied histopathologically using monkey jaws. When the surgical procedures were successful, the pulp tissues were kept alive, even though some slight atrophy and degeneration appeared. In some cases, however, severe reticular atrophy occurred which might be due to anaemia during operations.

Animals↗
PubMed1991

Effects of the penetration of a root canal filling material into the mandibular canal. 1. Tissue reaction to the material.

The root canal filling paste Vitapex (Neo Dental Chemical Products Co., Tokyo), made of calcium hydroxide and iodoform with the addition of silicone oil, was experimentally introduced into the mandibular canals of 29 dogs. Tissue reactions to the paste were examined by radiography, histopathology, and electron microscopy. These examinations revealed that the paste was phagocytosed by macrophages and, in general, was gradually resorbed over time. The paste was also found to cause heterotopic calcification and/or ossification within the area of original penetration.

Animals↗
PubMed1991

Effects of the penetration of a root canal filling material into the mandibular canal. 2. Changes in the alveolar nerve tissue.

The root canal filling material "Vitapex" (Neo Dental Chemical Products Co., Tokyo), made of calcium hydroxide and iodoform with the addition of silicone oil, was experimentally injected into the mandibular canals of dogs, and its effect on the alveolar nerve tissue was examined by light and electron microscopy. No change was observed as long as the injected paste did not come into direct contact with the nerve, but tissue damage was found at sites of direct contact. After removal of degenerated nerve debris by macrophages and Schwann cells, the persisting external membranes or "Schwann tubes" appeared to provide a scaffolding for axonal regeneration and Schwann cell proliferation.

Animals↗
PubMed1990

A rare variant of acinic cell carcinoma of the upper lip: a case report and review of the literature.

Acinic cell carcinomas of the lip are most uncommon. An exhaustive search of the Japanese- and English-language literature has revealed only 17 previously reported cases. One additional occurrence, a rare variant of this type of neoplasm, with mucous differentiation, that occurred in the upper lip of a 60-year-old man is described here. A brief review of the literature on this type of lip tumor is also given.

Carcinoma↗
PubMed1989

Studies on a novel antitumor antibiotic, phenazinomycin: taxonomy, fermentation, isolation, and physicochemical and biological characteristics.

A new antibiotic, phenazinomycin (C27H32N2O, MW 400), was isolated from the cultural mycelium of Streptomyces sp. WK-2057. This antibiotic possesses antibacterial activities against Gram-positive bacteria in vitro, direct cytotoxic activities against HeLa S3, P388 and P388 doxorubicin-resistant cells in vitro and antitumor activities against experimental murine tumors in vivo.

Animals↗
PubMed1987

Ultrastructural study of initial calcification in the rat subcutaneous tissues elicited by a root canal filling material.

The ultrastructural aspects of initial calcification in the rat subcutaneous tissues elicited by the root canal filling material Vitapex, made up of calcium hydroxide and iodoform with the addition of silicone oil, were studied by means of electron microscopic and cytochemical techniques. The embedded paste caused heterotopic calcifications. These were divided into two types: dystrophic calcification and matrix vesicle calcification which resembled that of bone tissue. These data suggest that Vitapex may be an excellent root canal filling material for differentiation of osteoblasts or cementoblasts from mesenchymal cells in the periodontal ligament.

Animals↗