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Biomedical subjects

S Fujihara

Publications and source records attributed to S Fujihara.

At least 55 records · Page 3Linked to original sources
PubMed1985

Erythroid differentiation of cultured murine erythroleukemia cells by the spermine analogue canavalmine.

Canavalmine, an analogue of spermine, induced erythroid differentiation of murine erythroleukemia cells 745A, as evidenced by benzidine staining and heme content of cultured cells. Benzidine-positive cells synthesizing hemoglobin appeared on day 4 after addition of 250 microM canavalmine. The canavalmine-induced cell differentiation was inhibited by the addition of agents which alter the structure of the cell membrane, such as local anesthetics (procainamide and lidocaine) or Ca2+ antagonists (nifedipine and verapamil) at dosages not toxic for the cell growth. Canavalmine did not significantly affect the levels of conjugated polyamines in the acid-insoluble fraction of the cells. In contrast, the level of free spermidine in the acid-soluble fraction greatly decreased during the 18 h after canavalmine treatment. Putrescine and spermidine, when added externally to the growth medium, showed dose-dependent inhibition of canavalmine-induced cell differentiation. Neither cadaverine nor spermine had any significant effect. These results suggest that not only structural change of cell membrane but alteration of the polyamine metabolism, especially a regulation of the cellular level of free spermidine, might have a key importance in erythroid differentiation of murine erythroleukemia cells induced by canavalmine.

Anesthetics, Local↗
PubMed1984

Effects of the spermine analogue canavalmine on proliferation of murine erythroleukemia cells in culture.

The effects of canavalmine, a structural analogue of spermine, were studied in cultured murine erythroleukemia cells 745A. Canavalmine exerted an inhibition on murine erythroleukemia cell growth at concentrations over 50 microM. The cell proliferation was, however, restored when canavalmine was removed from the culture medium after 24 h. Treatment of the cells with 500 microM canavalmine blocked the accumulation of intracellular polyamines. Especially, both spermine and spermidine levels were reduced below 50% of those in control cells after 48 h and below 30% after 96 h. The decreased contents of spermine and spermidine were compensated for by the increased content of canavalmine incorporated within the cells. In these cells, RNA and protein contents also decreased. The degree of growth inhibition by canavalmine during the cell cycle was examined using synchronized cells. Serum-induced growth stimulation was inhibited by canavalmine most effectively in the cells at G1 phase prior to DNA synthesis. The antiproliferative effect decreased when canavalmine was added to the cells after commencement of DNA synthesis. The results suggest that the growth-inhibitory action of canavalmine on murine erythroleukemia cells is most likely due to an inhibition of early events of the cell cycle, possibly due to the interference of a structure-specific function of spermidine and/or spermine on DNA replication.

Animals↗
PubMed1984

Lack of evidence for protein AA reactivity in amyloid deposits of lattice corneal dystrophy and amyloid corneal degeneration.

Amyloid fibrils occurring in primary and myeloma-associated (AL), secondary (AA), and certain neuropathic hereditary forms of systemic amyloidosis can be distinguished biochemically or immunohistologically as being composed of immunoglobulin light chain, protein AA, or prealbumin respectively. All types of systemic and several localized forms of amyloidosis contain amyloid P component (protein AP). We studied formalin-fixed tissue from eight cases of lattice corneal dystrophy by the immunoperoxidase method using antisera to proteins AA and AP, to normal serum prealbumin and prealbumin isolated from a case of hereditary amyloidosis, and to light-chain determinants; additional cases were examined by indirect immunofluorescence of fresh-frozen material. We found weak (1:10 dilution) staining with anti-AP, but no reactivity with other antisera. Congo red staining was resistant to pretreatment of sections with potassium permanganate, a characteristic of non-AA amyloid. Two-dimensional gels of solubilized proteins from frozen tissue from two cases of lattice corneal dystrophy resembled those obtained from normal human cornea. Western blots of two cases of polymorphous amyloid degeneration and solubilized protein from normal cornea did not react with radioactive iodine-labeled anti-AA or anti-AP with purified protein AP and unfixed protein AA amyloid tissue as controls. We were unable to corroborate the presence of protein AA in the amyloid deposits of lattice corneal dystrophy. Although staining with antiserum to protein AP was demonstrable, the molecular configuration of this protein in stromal deposits remains to be defined.

Amyloid↗
PubMed1984

Malignant cystosarcoma phyllodes of prostate.

A case of malignant cystosarcoma phyllodes of the prostate is reported in a 45-year-old male. This tumor was composed of benign columnar or squamous cystic folds and sarcomatous stroma including rhabdomyomatous elements. The prostatic origin of the tumor was clearly proved by the unlabeled immunoperoxidase method.

Autoantibodies↗
PubMed1984

Nature of amyloid deposits in hypernephroma. Immunocytochemical studies in 2 cases associated with amyloid polyneuropathy.

Two patients who presented with amyloid polyneuropathy were found to have an amyloid-positive hypernephroma. The amyloid extracted from the tumor of one patient was purified by gel filtration and found to immunoreact by immunodiffusion, only with antiserum against denatured lambda-type amyloid protein but not with antisera against denatured kappa amyloid, AA, or prealbumin. With the unlabeled immunoperoxidase method or immunofluorescence in combination with these specific antisera, it was shown that in both patients the amyloid deposits in the tumor, kidney, lymph node, muscle, and nerve had lambda-type amyloid antigenic fibril determinants. Some regions, amyloid-negative by congo red, immunoreacted with anti-lambda antiserum and were shown to represent amyloid fibrils electron microscopically. Several plasma cells found in the tumor and lymph node immunoreacted specifically with the anti-amyloid lambda antiserum. The findings provide the first observation that the amyloid in hypernephroma can be of immunocytic origin, even in the absence of overt signs of plasma cell dyscrasia, and suggest that amyloid polyneuropathy could be the presenting sign of hypernephroma.

Adenocarcinoma↗
PubMed1983

Determination of polyamines in human blood by electron-capture gas-liquid chromatography.

The present work was undertaken to develop a sensitive and selective method for the estimation of putrescine, spermidine and spermine in human blood employing electron-capture gas--liquid chromatography. Polyamines were derivatized with heptafluorobutyric anhydride. The heptafluorobutyric derivatives of polyamines could be well resolved within 15 min under a temperature programme. The detection limit was 0.1 pmol for putrescine and cadaverine, and 0.02 pmol for spermidine and spermine. The method was applied to polyamine determinations in erythrocytes from human blood. For pre-separation of the polyamines from other compounds, a simple clean-up method utilizing an activated Permutit has been devised. Major interfering substances could be removed by the batchwise Permutit treatment. The mean values of spermidine and spermine concentrations, and the spermidine/spermine ratio in erythrocytes obtained from normal subjects (n = 11) were similar to reported values. The analytical procedure is thought to be applicable to various biological materials.

Adult↗
PubMed1983

[Case of immunoblastic sarcoma (IBS)].

A patient with immunoblastic lymphadenopaty which evolved into immunoblastic sarcoma is reported. A 48-year-old female was admitted to our department because of cough and fever. A diagnosis of immunoblastic lymphadenopathy had been made two years before the present admission. Physical examination revealed generalized lymphadenopathy. Chest radiograms showed a left hilar mass. The pulmonary tumor and enlarged lymphnodes were treated by irradiation. Although there was marked improvement at first, she experienced several relapses. One year after the admission, chest radiograms showed multiple pulmonary lesions. She developed pancytopenia and bone metastases. At autopsy, the lymphnodes showed histological evidence of immunoblastic sarcoma; a pulmonary lesion showed immunoblastic lymphadenopathy.

Anemia, Hemolytic, Autoimmune↗
PubMed1982

Amyloid deposits in heart valves.

Localized amyloid deposits were found in 44% of the surgically removed heart valves. They are recognized only in severely destructed valves and the manner of the deposition closely resembles that of aortic intimal amyloid. Although the amyloid fibril protein of the chronically diseased valves is still unknown, it may be neither protein AA nor AL, and another type of amyloid is suggested.

Adolescent↗
PubMed1982

Amyloidosis associated with juvenile rheumatoid arthritis.

Clinical and pathological findings are reported in a Japanese girl who died of secondary amyloidosis associated with juvenile rheumatoid arthritis two years after the onset of symptoms. The patient had intermittent high fever, rheumatoid rash, polyarthralgia, and hepatosplenomegaly. The joints showed the typical histologic changes of juvenile rheumatoid arthritis. Amyloid deposition was found in various tissues; however, remarkable deposition of amyloid was observed in the gastrointestinal tract, especially in the ileum. The amyloid protein in this patient was identified as protein AA using the methods of potassium permanganate treatment and the peroxidase-antiperoxidase unlabeled antibody technique.

Amyloidosis↗
PubMed1982

Differentiation of amyloid fibril proteins in tissue sections. Two simple and reliable histological methods applied to fifty-one cases of systemic amyloidosis.

The potassium permanganate method and the unlabeled immunoperoxidase (PAP) method were applied for distinguishing different types of amyloid fibril proteins in conventionally fixed, paraffin-embedded tissue sections obtained from fifty-one autopsied cases of systemic amyloidosis and three control cases of well-analysed fibril proteins. All of the eighteen cases "sensitive" to permanganate treatment, whose amyloid deposits lost completely their affinity to Congo red and birefringence under polarized light, were shown to have AA antigenic determinants by the PAP method. Meanwhile, all of the remaining thirty-three "resistant" cases, where Congo red affinity and birefringence were retained at various degree even only in minimal areas, were negative for AA antigenicity. This indicated the feasibility of potassium permanganate method for the identification of AA protein based on this criterion of "sensitivity". Twenty-eight cases were classified as AA, A lambda, A kappa or AA+[A kappa] the remaining twenty-three cases were unclassified, and there were some discrepancies between the preliminary clinicopathological classification and the protein nature of the amyloid. It is important to differentiate the types of amyloid fibril protein of individual patients because the expedience of selective therapeutic approaches had been suggested. The two methods applied herein are handy and useful for this purpose.

Adult↗
PubMed1981

Primary localized amyloidosis of the genitourinary tract: immunohistochemical study on eleven cases.

Eleven cases of primary localized amyloidosis of the genitourinary tract were studied using the unlabeled immunoperoxidase method in combination with specific antisera against different chemical types of amyloid fibril proteins (AA,A lambda, and A kappa). Nine of these were shown to have A lambda antigenic fibril determinants, suggesting that the fibrils of most, if not all, of these cases are of immunocytic origin.

Adult↗
PubMed1981

Congophilic angiopathy in the pathogenesis of Alzheimer's degeneration.

The pathologic manifestations in 45 valid cases of Alzheimer's degeneration were evaluated with specific focus on the presence of Congophilic angiopathy. The latter lesion was found in 89% of the cases and had a definite temporal relationship to the presence of neuritic plaques and neurofibrillary tangles. Two types of plaques were defined, the neuritic and perivascular. The latter was associated spatially with vessels, but not neurofibrillary tangles, while the converse was true of the neuritic plaques. Based on the pattern of mural involvement of vessels, distinctions could be made to differentiate morphologically between cerebral and peripheral amyloid angiopathy. Alzheimer's degeneration appears to be a disease complex with a variety of etiologies. Little or no differences in morphologic characteristics between the presenium and senium process could be discerned. Congophilic angiopathy appears to be a major feature in the pathogenesis of Alzheimer's degeneration.

Aged↗
PubMed1980

Asparagine formation in soybean nodules.

(15)NH(4) (+) and [(15)N](amide)-glutamine externally supplied to detached nodules from soybean plants (cv. Tamanishiki) were incorporated within nodule tissues by vacuum infiltration and metabolized to various nitrogen compounds during 60 minutes of incubation time. In the case of (15)NH(4) (+) - feeding, the (15)N abundance ratio was highest in the amide nitrogen of glutamine, followed by glutamate and the amide nitrogen of asparagine. In (15)N content (micrograms excess (15)N), the amide nitrogen of asparagine was most highly enriched after 60 minutes. (15)NH(4) (+) was also appreciably assimilated into alanine.When [(15)N](amide)-glutamine was fed to detached nodules, (15)N was almost entirely in the amide nitrogen of asparagine. The result indicates the presence of active glutamine-linked asparagine synthesizing systems in soybean root nodules.

Journal Article↗
PubMed1979

Effects of dimethyl sulfoxide and colchicine on the resorption of experimental amyloid.

The induction of amyloid in C3H mice by either casein solution or complete Freund's adjuvant emulsion with Mycobacterium butyricum was confirmed by partial splenectomy. The animals were autopsied after treatment with dimethyl sulfoxide (550 mg/kg, 50 times), colchicine (0.02 mg/kg, 15--37 times), or saline solution as a control. Detailed histological comparisons of biopsy and autopsy spleens provided evidence that dimethyl sulfoxide was significantly effective in the resorption of amyloid, while in the animals treated with colchicine amyloid deposition was increased. The effect of dimethyl sulfoxide was discussed with reference to the modification of amyloid fibrils.

Amyloid↗