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Biomedical subjects

S Fujimi

Publications and source records attributed to S Fujimi.

69 records · Page 4Linked to original sources
PubMed1980

Comparison of hemodynamics induced by conventional acetate hemodialysis, bicarbonate hemodialysis and ultrafiltration.

Six patients on conventional acetate hemodialysis who had a marked decrease in total peripheral resistance index (TPRI) and hypotension after dialysis were selected for study. Systemic hemodynamic factors such as cardiac output, blood pressure, hematocrit and heart rate were measured, and the values were compared to those found after bicarbonate hemodialysis (Bi-HD) or ultrafiltration without simultaneous dialysis (UF) in the same patients. After Bi-HD and UF, the hemodynamic indices remained stable. Hematocrit level was significantly elevated after Bi-HD (+2.7%, P less than 0.05). Heart rate was insignificantly increased after each procedure. We concluded that Bi-HD can prevent unpleasant side effects induced by the decrease in TPRI with acetate hemodialysis. The stability of TPRI resulting from the increase in hematocrit level during Bi-HD may contribute to the prevention of cardiovascular instability.

Acetates↗
PubMed1977

Purification and properties of urinary alkaline ribonucleases from patients with nephrotic syndrome.

Four urinary alkaline ribonucleases (RNase, EC 3.1.4.22) were purified from patients with nephrotic syndrome using phosphocellulose, DEAE-cellulose and Sephadex G-75 chromatographiy. These enzymes were designated as RNases 1--4, respectively, in order of elution on phosphocellulose chromatography. The respective purification of each fraction was 41-, 23-, 34- and 27-fold with a total recovery of 25%. The pH optima of these RNases were around 8.5 with Tris/HCl buffer and the reaction was activated by mono- and divalent cations, such as Na+, K+, Mg2+ and Ca2+, but inhibited by Fe2+, Cu2+ and Zn2+. EDTA had little effect on the velocity of reaction. The molecular weights of RNases 1--4 were estimated by gel filtration as 45 000, 32 000, 20 000, and 13 000, respectively. Each enzyme hydrolyzed pyrimidine nucleotides preferentially with higher affinity for poly(C) than poly (U) as determined with synthetic polymers and was free from other nucleolytic enzymes. The patients with renal disorders excreted one to four RNases in urine and the number of enzymes increased as the concentration of urinary protein increased. On the other hand, normal subjects excreted a single fraction essentially identical to RNase 1.

Adolescent↗
PubMedDate not supplied

Blood concentration and urinary excretion of captopril (SQ 14,225) in patients with chronic renal failure.

Blood concentration and urinary excretion of captopril following 50 mg oral administration were determined by high-performance liquid chromatography in normal subjects and patients with chronic renal failure. In normal subjects, the maximum blood concentration of the free form of captopril was obtained within 1 hour and was not detectable after 6 hours; 41% of administered captopril was excreted into the urine as free form and metabolites within 2 hours, and 58% within 6 hours. In chronic renal failure patients with an average serum creatinine of 5.1 mg/dl, the absorption constant (Ka), maximum concentration (Cmax), and area under the blood concentration curve (AUC) were not significantly different from those in the normal subjects, but the elimination constant (Ke) and biological half-life (T1/2) showed a significant delay in the disappearance of captopril from the blood (p less than 0.01 respectively). The cumulative amount of urinary excretion of either free-form captopril or its' metabolites was significantly decreased at 2, 4, and 6 hours in chronic renal failure patients (p less than 0.01 or less, respectively). Impairment of kidney function is suggested to be an important factor in the promotion of blood retention of captopril.

Adult↗