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Biomedical subjects

S Fujimura

Publications and source records attributed to S Fujimura.

At least 307 records · Page 17Linked to original sources
PubMed1990

A new method for estimating filtration variables in isolated zone 1 rat lung.

The filtration variables, K (filtration coefficient), Ppmv (perimicrovascular pressure) and sigma (reflection coefficient), were estimated independently in previous reports using the Starling equation or the micropuncture method. We used matrix algebra to estimate these variables simultaneously. We measured filtration rate (Q) by a gravimetric method in isolated rat lung lobes in zone 1 conditions (alveolar pressure = 20 cmH2O) at two vascular pressures, Pvasc = 15 or 18 cm H2O and perfused the lobes with plasma containing a low or a high concentration of protein. By extrapolating the log of the rate of weight gain to t = 0, we obtain the initial filtration rate before any of the pressure variables (microvascular and perimicrovascular hydrostatic pressures) in the Starling equation changed. Assuming that protein filtered into perimicrovascular space only by convection, we substituted it into the Starling equation as follows: Q = K [(Pmv -- Ppmv) -- sigma 2 (IImv)], where Pmv and IImv are microvascular and perimicrovascular plasma protein osmotic pressures. IImv was estimated by Yamada's equation (Yamada et al. 1985). For the matrix algebra, we used three values, we omitted the value for the high protein, low vascular pressure experiment. We obtained K = 26.3 [mg/(min x cmH2O x g wet weight)], Ppmv = 6.2 cmH2O and sigma = 0.46. These values agree with values from previous reports. Since these 3 filtration variables are interrelated, this new method for simultaneous measurement is more accurate than independent measurements are. The chief advantage of this method is that it does not require a separate estimate of isogravimetric pressure or a direct measurement of interstitial pressure, and all variables are obtained simultaneously.

Algorithms↗
PubMed1990

A new selective, differential agar medium for isolation of Vibrio cholerae O1: PMT (polymyxin-mannose-tellurite) agar.

A new selective and differential agar medium, polymyxin-mannose-tellurite (PMT) agar was devised to differentiate easily colonies of Vibrio cholerae O1 from those of V. cholerae non-O1. The differentiation between colonies of the two vibrios is based on mannose-fermentation. Colonies of V. cholerae O1 on the agar are agglutinated with O1 antiserum of V. cholerae much more easily than those on thiosulfate-citrate-bile salts-sucrose (TCBS) agar.

Agar↗
PubMed1990

[OKT4 epitope deficiency in a patient with thymoma and hypogammaglobulinemia].

A 61-year-old male stonecutter was in excellent health until December 1988 when he was admitted to Miyagikenritsu Semine Hospital with an abnormality on chest X-ray film. Chest X-ray film revealed a left anterior mass that was confirmed on chest CT scan and MRI. Biochemical examinations and serum protein electrophoresis demonstrated hypoproteinemia (5.4 g/dl) and hypogammaglobulinemia (7.9%, 0.43 g/dl). Lymphocyte subset analysis showed OKT4 epitope deficiency (OKT4 0.1% and Leu3a 28.4%), and decrease of CD4/CD8 ratio (0.65). An encapsulated anterior mediastinal mass and the thymus were resected on January 9, 1989, without difficulty. Histology revealed a thymoma with a spindle cell epithelial component. OKT4 epitope deficiency in a patient with Good's syndrome (thymoma with hypogammaglobulinemia) was our diagnosis. There was no change in the patient's serum immunoglobulin level after thymectomy. Fifteen cases of Good's syndrome have been reported in Japan, but this is the first report of OKT4 epitope deficiency with Good's syndrome in Japan.

Agammaglobulinemia↗
PubMed1990

[Cytotoxicity of lymphocytes sensitized by autologous tumor cells in vitro, against autologous lung cancer cell lines].

In order to obtain killer lymphocytes that would have a strong cytotoxicity against autologous tumor cells, we conducted a cytotoxic assay of effector cells cultured from human peripheral blood lymphocytes (PBL) using 10 cultured lung cancer cell lines as target cells. We present this result. (1) Lymphocytes obtained by 17 days mixed lymphocyte tumor cell culture (MLTC: Fresh PBL from lung cancer patients was mixed with irradiated autologous tumor cells in a medium, and cultured for 3 days. Irradiated allogeneic lymphocytes were then added, and cultured for 14 days) were cytotoxic against 4 of the 10 autologous lung cancer cell lines. (2) Lymphocytes obtained by 14 days MLTC and 3 more days culture in a medium containing interleukin 2 were cytotoxic against all 10 of the autologous lung cancer cell lines. These lymphocytes proliferated to 4.13 times of the original cell number, and their surface marker was OKT3+. These killer lymphocytes are expected to be effective in adoptive immunotherapy as effector cells.

Adult↗
PubMed1990

[Clinicopathologic assessment of esophageal cysts--a report of 8 cases].

We experienced 8 cases of esophageal cysts on between January, 1965 and March, 1989. During the same period, 320 cases of mediastinal tumors were operated in our department, and esophageal cysts were 2.5% of these cases. They were 7 males and 1 female, and ranged in age from 5 to 51 (mean 29.9) years. The cysts were located in the right in 6 and in the left in 2, and in the upper third of the esophagus in 1, middle third in 3 and lower third in 2 cases. A correct preoperative diagnosis was made in only 1 case. The definite diagnoses were not obtained from thoracic CT scans and esophagography. We tried transesophageal ultrasonic endoscopy in the recent 2 cases, and could diagnosed in 1 case as a cyst in the esophageal wall. The another case, we could not diagnose, was a cyst attached to the esophageal wall by a muscular stalk. We experienced 2 cases of post operative bleedings, so we must be careful of hemostasis of the muscular layers after enucleation of the cysts. Histological examinations of the cysts showed ciliated columnar epithelium in all cases. Cartilage were observed (bronchogenic cysts) in 4 patients (50%) and two muscle layers were observed (duplication cysts) in 4 other patients (50%). But 2 cases of bronchogenic cysts had two muscular layers, 1 case of them was well-defined. And 1 case of duplication cyst had poor two muscular layers. We cannot divide clearly the esophageal cysts into bronchogenic cyst or duplication cyst, in the point of having cartilage and double muscle layers.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1990

[Activated pulmonary intravascular macrophages increase pulmonary microvascular permeability in sheep].

Pulmonary intravascular macrophages (PIMs) reside in the alveolar capillaries of sheep lung. However, the role of PIMs activation in the mechanism of increasing pulmonary microvascular permeability is not clear. We determined, using awake sheep with chronic lung lymph fistulas, the effects of intraarterial infusion of latex beads emulsion (1 microns in diameter) on lung lymph-dynamics. Sheep were divided into 3 groups; control, low dose latex and high dose latex groups. In control group, their lymph-dynamics were stable during the experimental period. In low dose latex group (5.46 x 10(9) beads/kg), lung lymph flow increased during the infusion period, but lung lymph protein clearance did not change. In high dose latex group (5.46 x 10(10) beads/kg), lung lymph flow began to increase during the infusion period and remained high, and lung lymph protein clearance increased significantly during and after the infusion of latex beads emulsion. The latex beads mainly distributed to lungs, and 70% of the beads were caught by PIMs in their phagosomes. These findings suggest that the activation of PIMs through their phagocytic uptake of circulating microparticles increases pulmonary microvascular permeability.

Animals↗
PubMed1990

[Pleuropneumonectomy with thymectomy for invasive thymoma with pleural disseminations--a case report].

A case of invasive thymoma with pleural disseminations, treated with pleuropneumonectomy and thymectomy , is presented. A 30-year-old woman was admitted with abnormal shadows in left lung field and mediastinum. On chest CT examination, the tumor shadows were located anterior mediastinum and left chest wall. Thymoma with pleural disseminations was the most likely consideration by the needle biopsy specimen of the tumor under left chest wall. Preoperative radiotherapy for anterior mediastinum mass was effective. Pleuropneumonectomy and thymectomy combined with partial resections of intercostal muscles and diaphragm were performed. Histologically, the tumor was arranged in lobular structure, composed of epithelial cells, with poorly infiltration of lymphocytes, and invaded into intercostal muscles or lung. Postoperative radiotherapy for mediastinum and left chest wall was added. Tumors may be removed completely by pan-pleuropneumonectomy and thymectomy for invasive thymoma with pleural disseminations.

Adult↗
PubMed1990

[Analysis of patients with resected small-size (less than or equal to 2 cm in diameter) peripheral type lung cancer lesions].

From 1953 to 1985, a total of 1289 patients with primary carcinoma of the lung underwent surgical treatment. Of these 116 (8.9%) had small-sized (less than or equal to 2 cm in diameter) peripheral type lung cancer lesions. This study had three purposes: 1) to analyse how small-sized lung cancer lesions were detected; 2) to evaluate the reliability of diagnosis of small-sized cancer lesions; and 3) to evaluate pre- and post-prognostic factors of such patients compared with patients with peripheral type lung cancer lesions 2.1-3 cm in diameter. Of the 115 patients with small-sized lung cancer lesions were detected in the course of mass surveys. Cytopathological diagnosis in 75% of the patients resulted from transbronchial brushing cytology. The 5-year survival rate of patients who underwent resection of small-sized peripheral type lung cancer lesions was 70% (2.1-3 cm; 52%). Various factors such as histologic type, nodal involvement, pleural involvement, pathological stage, and success of the operation were shown to significantly affect survival. A comparison of two groups, i.e., those with lesions smaller than 2 cm in diameter and those with lesions 2.1-3 cm in diameter, showed the rate of lymph node metastasis to be significantly different. Of the patients with peripheral lung cancer lesions smaller than 2 cm who underwent surgery, 21% had peribronchial, hilar, or mediastinal lymph node metastasis. On the other hand, lymph node metastasis was seen in 43% of cases with peripheral lung cancer lesions 2.1-3 cm in diameter who underwent surgery.

Humans↗
PubMed1990

[Treatment of invasive thymoma with myasthenia gravis: a case report responsive to azathioprine and methylprednisolone].

The treatment of invasive thymomas associated with autoimmune diseases, such as myasthenia gravis (MG) and pure red cell aplasia, has not been established. In this paper, we report a 37-year-old patient with post thymectomy myasthenia gravis, who underwent subtotal resection of invasive thymoma with residual lesions of intrathoracic implants. He was treated with azathioprine (AZP) and methylprednisolone (MP). During two months from the start of this therapy, the clinical symptom was improved apparently and the recurrent tumor was reduced. Lasting 18 months of the treatment, he had to stop it because of the renal failure, but now he has no sign of the recurrence. Further studies with this combined regiments are warranted in the treatment of the invasive thymomas associated with autoimmune disease.

Adult↗
PubMed1990

[A useful combination chemotherapy of cisplatin and etoposide in mediastinal yolk sac tumor].

A 28-year-old male was referred to our hospital because of chest pain and dry cough. Chest x-ray film revealed a tumor mass in the anterior mediastinum indicating invasion into the chest wall and upper lobe of right lung. No tumor was found in the testis. Serum level of alpha-fetoprotein (AFP) was 6,400 ng/ml. Percutaneous biopsy of the tumor suggested yolk sac tumor. The patient was treated with a combination chemotherapy schedule including 30 mg of cisplatin (CDDP) i.v. on Days 1, 2, 3, 4, and 5 and 150 mg of etoposide on Days 1, 2, and 3 as one course therapy. Two courses were employed. Partial response was achieved followed by successful resection of the tumor. This case indicated that a combination chemotherapy CDDP and etoposide combined with surgery could play an active role increasing long-term survival rates in mediastinal yolk sac tumors.

Adult↗
PubMed1990

[A case of thoracic neurilemoma with neurilemomas of the upper limb and sacrum].

A 64-year-old male was admitted in February 24, 1988 because of an abnormal shadow on chest X-ray. The tumor in the thoracic wall was removed at the thoracotomy and histology showed neurilemoma. Aspiration biopsy of tumors on the left upper limb and sacrum revealed the typical histopathologic appearance of neurilemomas. The possibility of malignant transformation and sequential appearance of tumors in patients with a neurilemoma indicates that complete resection and thorough follow-up examination are important.

Humans↗
PubMed1990

[A study of lung cancer with presence of pleural effusion at the time of thoracotomy--cytologic evaluation of a relatively small amount of pleural effusion and prognosis after removal].

The cytologic evaluation of a relatively small amount of pleural effusion in lung cancer at the time of thoracotomy has not been previously considered, and prognosis after removal has not been clarified. In order to clarify these points, 99 cases of removed lung cancer with presence of pleural effusion at the time of thoracotomy were examined following cytology. Our study was conducted with regard to the amount and nature of pleural effusion, pleural effusion cytology, tumor development and its relation to prognosis. On the basis of this study a formula was developed relating the occurrence rate of cytologically positive pleural effusion with the development of tumor employing multivariate analysis, specifically the multiple regression analysis. 21% of these cases showed cytologically positive pleural effusion, indicating an absence of its correlation to the amount of pleural effusion; the conducting of cytology regardless of the amount of pleural effusion was found to be significant in determining the precise stage. The occurrence rate of cytologically positive squamous cell carcinoma was significantly few compared with those in other cell types. Regarding the pleural effusion of a relatively small amount, the prognosis after removal showed a relatively high three-year survival rate of 32% for those cases with negative pleural metastasis despite the positive showing of pleural effusion cytology, indicating the viability of surgery. From the occurrence rate of cytologically positive pleural effusion (Y) and the degree of pleural metastasis (X1)/the degree of pleural invasion (X2)/the degree of lymph nodes metastasis (X3), the following formula was obtained employing multiple regression analysis: Y = 0.344X1 + 0.050X2 + 0.034X3 + 0.075 (proportion 0.840).(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1990

[A case of mediastinal yolk sac tumor successfully treated with chemotherapy and surgery].

A 28-year-old man was admitted due to increasing respiratory symptoms. X-ray examination of the chest showed a tumor mass in the anterior mediastinum with possible invasion into the chest wall and upper lobe of right lung. No tumor was found in the testis. Serum alpha-fetoprotein (AFP) concentration was 6400 ng/ml. Serum levels of CEA and HCG were within normal limits. Percutaneous biopsy of the tumor strongly suggested yolk sac tumor with an evidence of AFP by an immunoenzyme labelling technique. The serum AFP rapidly decreased after two courses of combination chemotherapy. En bloc resection of the tumor was successfully performed and third chemotherapy was added. Mediastinal yolk sac tumors should be treated with combination chemotherapy and surgical resection.

Adult↗
PubMed1990

[Surgical experience of bronchogenic early squamous cell carcinoma detected by sputum cytology in lung cancer screening].

Eighty-one patients of roentgenologically occult lung cancer (all men, and squamous cell carcinoma) were detected by sputum cytology in lung cancer screening of "Miyagi Program". Sixty-seven patients were resected surgically, and sixty-four of them underwent absolutely curative resection. In fifty-six patients, carcinoma did not penetrate the bronchial wall, and all of them were free from lymph node involvement. In eleven patients, carcinoma penetrated the bronchial wall, and three of them were proved to have lymph node involvement. For that reason, carcinoma which did not penetrate the bronchial wall and was free from lymph node involvement, was defined as early squamous cell carcinoma. Fourty-two early squamous cell carcinoma were located on segmental or more proximal bronchi, but twelve were located on subsegmental or more peripheral bronchi. Five-year survival of fifty-six patients with early squamous cell carcinoma were 91.8%, seven of fifty-six early squamous cell carcinoma patients were multicentric, which were detected synchronously in four cases, postoperatively in two cases, and both synchronously and postoperatively in one case. Two of three postoperatively detected cases were resected surgically and alive without cancer. These results indicate the validity of surgical treatment for roentgenologically occult squamous cell carcinoma. As the most serious prognostic problem is multicentricity, careful localization of primary lesion and postoperative intensive follow-up should be considered.

Aged↗
PubMed1989

Co-purification of thymidylate kinase and cytosolic thymidine kinase from human term placenta by affinity chromatography.

It was revealed that thymidylate kinase was purified together with cytosolic thymidine kinase from human term placenta by p-aminophenyl thymidine-3'-phosphate-CH-Sepharose affinity column chromatography, which has been commonly used for purification of thymidine kinase. In addition, it was noted that mitochondrial thymidine kinase and nucleoside diphosphate kinase were concurrently eliminated. In the presence of ATP, cytosolic thymidine kinase and thymidylate kinase could be separated from each other by Ultrogel AcA 34 filtration, and their molecular weights were estimated to be 70,000 and 50,000, respectively. On SDS-polyacrylamide gel electrophoresis, thymidine kinase protein exhibited a band of 26,000, which was compatible with the molecular weight of the enzyme subunit calculated from its cDNA, while thymidylate kinase protein showed 24,000. Thymidylate kinase could utilize either ATP or dATP as an efficient phosphate donor, and showed substrate specificity for dTMP.

Adenosine Triphosphate↗
PubMed1989

Multiple forms of proteases of Bacteroides gingivalis and their cellular location.

Protease of Bacteroides gingivalis ATCC 33277 was found in intracellular membrane-free, intracellular membrane-bound and extracellular fractions. The insoluble form of proteases was solubilized with a detergent. The elution patterns of proteases on gel filtration in each fraction were rather different. However, enzymatic properties of the proteases separated by gel filtration were very similar.

Bacteroides↗
PubMed1989

A study of preservation solution for 48- and 96-hour simple hypothermic storage of canine lung transplants.

Forty-eight-hr and 96-hr simple hypothermic preservations of the lung were studied on dogs using 4 kinds of solutions resembling extracellular electrolyte compositions (Ep1-Ep4). The pH of each solution was changed from 6.377 to 7.463 by HPO4(2-)/H2PO4- ratio. Twenty dogs were prepared as donors (12 for 48-hr preservation with Ep1, Ep2 and Ep3, and 8 for 96-hr preservation with Ep3 and Ep4, respectively). The heart-lung block was removed from the donor, and flushed with 500 ml of preservation solution via the pulmonary artery, then immersed in the same cold (4 degrees C) solution, after which it was stored in a cold (4 degrees C) room. After preservation, the left lung was separated from the heart-lung block and was transplanted into the recipient orthotopically. The function of the transplanted lung was evaluated with serial x-ray findings, contralateral pulmonary artery ligation test, and histologic findings. In the case of 48-hr preservation, all of the four recipients with Ep3 (pH: 7.225) showed good aeration of the grafted lungs on the chest x-ray in the early postoperative period. As for the 96-hr preservation cases, one with Ep3 and three with Ep4 (pH: 7.463) presented good x-ray findings. Of these successful cases, seven dogs (5 of 48-hr preservation cases, six dogs tolerated this procedure. On histopathological examination of the successful cases, the 48-hr preservation cases showed almost normal pulmonary structure, while the 96-hr preservation cases showed slight degenerative changes in the alveolar walls. From the present study, it becomes apparent that clinical application of 48-hr preservation may be possible by using a phosphate buffered extracellular solution.

Animals↗