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Biomedical subjects

S Fujimura

Publications and source records attributed to S Fujimura.

At least 343 records · Page 19Linked to original sources
PubMed1987

Histologic assessment of bronchial anastomotic healing in canine lung transplantation.

Postoperative wound healing of the bronchial anastomosis was studied in dogs with autotransplantation (20 dogs, 7 days to 6 years postoperatively) and allotransplantation (62 dogs, 5 to 174 days postoperatively) of the left lung. In the group undergoing lung allotransplantation, the relationship among three histologic parameters was studied: the grade of lung allograft rejection, the degree of changes in the epithelium, and submucous lymphocyte infiltration along the donor bronchus within approximately a 0.5 cm area distal to the anastomosis. In lung autotransplantation, mucosal continuity began to be observed 1 week postoperatively. Mucosal continuity and apparent collagen formation on any bronchial contiguous site were demonstrated in most animals studied more than 3 weeks postoperatively. Bronchial anastomotic healing tended to be slower in lung allotransplantation than in autotransplantation, although a mucosal continuity at the anastomosis was sporadically observed in immunosuppressed dogs surviving more than 3 weeks postoperatively with a lung allograft. There were significant rank correlations among the three histologic parameters, which showed that lung allograft rejection is closely connected with wound healing of the bronchial anastomosis in lung allotransplantation. Meticulous mucosal approximation is most necessary during bronchial anastomotic procedures. Establishment of an exact method for early monitoring of lung allograft rejection is absolutely necessary for lung allotransplantation.

Animals↗
PubMed1987

Results of surgical treatment for thymoma based on 66 patients.

Sixty-six patients with thymoma have undergone surgical treatment since 1965 and have been assessed from the viewpoint of clinical manifestations and prognosis. Thirty-one patients with encapsulated thymoma were treated with total surgical resection alone, and they had no postoperative tumor recurrence. With the exception of one patient who died of respiratory insufficiency on the fourth day after the operation, 34 patients with invasive thymoma were evaluated on the basis of their postoperative prognosis. Fifteen patients with invasive thymoma died from 1 1/2 months to 10 years, 1 month postoperatively; 9 died of local or metastatic tumor and 6 died of other diseases. Associated autoimmune diseases, as well as the invasive tendency of the tumors, apparently affected the prognosis. Ten-year survival rates of the patients who underwent surgical treatment were as follows: 61.6% for the total group, 74.3% for those with encapsulated thymoma, and 49.4% for those with invasive thymoma. In the surgical treatment for invasive thymomas, one should aim to resect the tumor totally, even though adjacent tissues are resected simultaneously. Even for the patient with total resection of invasive tumor, postoperative radiation should be required. Finally, if residual tumor must be left during the operation, postoperative radiation as well as anticancer chemotherapy should be aggressively scheduled, because postoperative distant metastasis may appear in these patients with residual thymoma.

Adolescent↗
PubMed1986

Isolation and characterization of a peptidase from an oral strain of Corynebacterium matruchotii.

From the cell extract of Corynebacterium matruchotii strain ATCC 14266, a peptidase could be isolated and purified, increasing the specific activity 267 times. This enzyme with a molecular weight of 60,000 was completely inactivated by heating at 50 degrees C for 20 min, its optimum pH was found to be pH 7.5 and the isoelectric point was 4.1. The peptidase was inhibited by diisopropylfluorophosphate, phenylmethanesulfonyl fluoride, tosyl-L-lysine chloromethyl ketone, chymostatin and urea. Among various synthetic substrates tested, only benzoyl-L-arginine-p-nitroanilide and benzoyl-L-arginine ethylester were found to be hydrolyzed by this enzyme. Several proteins investigated were not hydrolyzed, but the enzyme inactivated a peptidic staphylococcal bacteriocin.

Bacteriocins↗
PubMed1986

A rat model for monitoring homograft rejection of the lung.

A new surgical procedure was devised for the experimental homograft of the lung. The pulmonary artery and vein and bronchus were anastomosed under the operation microscope using interrupted sutures in group W (30 outbred Wistar rats) and continuous sutures in group F (7 inbred F344/Ducrj Rats). Mean ischemia time during the operation was 95.3 +/- 10.3 min in the group W and 55.6 +/- 5.8 in group F. Eight out of 30 rats (27%) of group W and all rats of group F survived more than 24 hr after the surgery. Pulmonary functions of grafted lungs were evaluated by serial x-rays and the perfusion ratio was 27.4 +/- 11.6% (mean +/- S.D.) in group W and 25.4 +/- 7.7% in group F. A patent opening of the hilar anastomosis in these rats was confirmed at autopsy. This model with continuous suture would be useful for the rat lung transplantation.

Animals↗
PubMed1986

[Results and prevention of complications in bronchoplastic surgery in lung cancer].

Fifty-eight patients have undergone bronchoplastic surgery for lung cancer and were reviewed in an attempt to evaluate its postoperative survival rate and to establish a certain method to prevent postoperative complications. Five-year survival rates of the patients with the reconstructive surgery were 36.9, 66.6, 7.9 per cent in over-all patients, stage I and II patients, and stage III patients, respectively. These results can compare favorable with the results obtained in patients who underwent standard operation for lung cancer. Seven of 9 patients with sleeve lobectomy and pulmonary artery reconstruction died within 2 years and 7 months postoperatively, and 4 of them showed distant metastasis. This type of operation may be alternative to pneumonectomy only when pneumonectomy would not be tolerated because of low cardiopulmonary reserve. Six of 8 patients died within 3 years after carina reconstruction. In this type of operation 4 cases showed tumor relapse around anastomotic site. Including 4 patients with carina reconstruction, 8 cases revealed postoperative local tumor recurrence despite the tumor free bronchial stump by frozen sections. These results indicate that prophylactic radiation therapy may be necessary to prevent local tumor relapse after the reconstructive surgery for the advanced lung cancer patients.

Adenocarcinoma↗
PubMed1985

Uridine diphosphate reductase of Ehrlich ascites tumor is insensitive to hydroxyurea.

Uridine diphosphate (UDP) reductase was isolated in the supernatant fraction obtained after the acidification of the cytosol of Ehrlich ascites tumor cells, and was found insensitive to 10 mM hydroxyurea. However, cytidine diphosphate (CDP) reductase, being separated concurrently in the precipitate fraction, was readily inhibited. In the cytosol fraction of either Ehrlich ascites tumor, Yoshida ascites sarcoma or regenerating rat liver after partial hepatectomy, UDP reduction activity, in contrast to CDP reduction activity, is not sensitive to hydroxyurea.

Animals↗
PubMed1985

Purification and properties of a proline iminopeptidase from Propionibacterium acnes.

Proline iminopeptidase was extracted from the cells of a strain of Propionibacterium acnes and purified. The molecular weight was estimated to be about 120,000 by SDS-polyacrylamide gel electrophoresis. The enzyme showed the highest activity at 50 degrees C-55 degrees C and its optimum pH was found at 7.5-8.0. The enzyme activity was inhibited by p-chloromercuribenzoate, indicating that this peptidase is a SH-enzyme. Especially prolyl-glycyl-glycine but also prolyl-proline bonds were hydrolyzed by this enzyme, glycyl-proline was not split.

Aminopeptidases↗
PubMed1985

Experience with surgery for thymoma associated with pure red blood cell aplasia. Report of three cases.

We present three cases of thymoma associated with pure red blood cell aplasia in which thymomectomy and thymectomy were performed. Case 1, a patient with pure red blood cell aplasia and hypogammaglobulinemia, was treated after surgery with immunosuppressive agents. She did not show any remission and died eight months after the operation. Case 2, a patient with pure red blood cell aplasia alone, showed transient erythropoiesis only in the early post-operative period and died one year and seven months after the operation. Patient 3 had pure red blood cell aplasia alone before surgery and was treated after surgery with prednisolone and fluoxymesterone. He showed good remission from the aplasia after these treatments; however, myasthenia gravis appeared seven months after the operation. These results seem to show that such combined therapy as applied in case 3 may be effective for some of the patients with pure red blood cell aplasia and thymoma; however, the effects of thymomectomy or thymectomy (or both) are still controversial for the treatment of pure red blood cell aplasia.

Anemia, Aplastic↗
PubMed1985

Prognostic evaluation of tracheobronchial reconstruction for bronchogenic carcinoma.

Fifty-two patients have undergone tracheobronchial reconstruction for bronchogenic carcinomas over a 20 year period and have been evaluated from the view point of prognosis. Five-year survival rates of the patients undergoing reconstructive operations were as follows: 35% for the total group, 50% for those with squamous cell carcinoma, and 64% for those with Stage I and II disease. No patients with adenocarcinoma or Stage III disease have survived more than 5 years. However, the number of patients with early adenocarcinoma was too small for us to conclude that the histologic type per se affected survival. Six of eight patients with sleeve lobectomy and pulmonary artery reconstruction died within 2 years, 7 months postoperatively. Five of seven patients died within 1 year after carinal reconstruction. However, two are alive at 4 months and 2 years, 9 months after left or right sleeve pneumonectomy. In summary, any types of lobectomy or pneumonectomy with reconstruction of the tracheobronchial tree can be conducted in patients with Stage I and II lung cancer. Sleeve lobectomy with pulmonary artery reconstruction can be an alternative to pneumonectomy when pneumonectomy is contraindicated because of low cardiopulmonary reserve. In patients undergoing reconstruction of the carina, prophylactic radiation therapy may be necessary during the postoperative course.

Adenocarcinoma↗