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S Fujisaki

Publications and source records attributed to S Fujisaki.

At least 73 records · Page 4Linked to original sources
PubMed1990

Cloning and nucleotide sequence of the ispA gene responsible for farnesyl diphosphate synthase activity in Escherichia coli.

The molecular cloning and the determination of the nucleotide sequence of the ispA gene responsible for farnesyl diphosphate (FPP) synthase [EC 2.5.1.1] activity in Escherichia coli are described. E. coli ispA strains have temperature-sensitive FPP synthase, and the defective gene is located at about min 10 on the chromosome. The wild-type ispA gene was subcloned from a lambda phage clone containing the chromosomal fragment around min 10, picked up from the aligned genomic library of Kohara et al. [Kohara, Y., Akiyama, K., & Isono, K. (1987) Cell 50, 495-508]. The cloned gene was identified as the ispA gene by the recovery and amplification of FPP synthase activity in an ispA strain. A 1,452-nucleotide sequence of the cloned fragment was determined. This sequence specifies two open reading frames, ORF-1 and ORF-2, encoding proteins with the expected molecular weights of 8,951 and 32,158, respectively. A part of the deduced amino acid sequence of ORF-2 showed similarity to the sequences of eucaryotic FPP synthases and of crtE product of a photosynthetic bacterium. The plasmid carrying ORF-2 downstream of the lac promoter complemented the defect of FPP synthase activity of the ispA mutant, showing that the product encoded by ORF-2 is the ispA product. The maxicell analysis indicated that a protein of molecular weight 36,000, approximately consistent with the molecular weight of the deduced ORF-2-encoded protein, is the gene product.

Alkyl and Aryl Transferases↗
PubMed1990

Immunoregulatory activity in human decidua.

The immunoregulatory factor(s) that suppresses normal human peripheral blood mononuclear cells (PBM) function was extracted from the decidua of pregnant women (8-13 weeks). Compared with extracts from the proliferative endometrium, the decidual extracts from fresh surgical specimens markedly inhibited 3H-thymidine incorporation by PBM in response to T-cell mitogens. The majority of the lymphocyte-inhibitory activity was found to be associated with a material of large molecular weight in the decidua, as estimated by Sephadex G-200 filtration. Immunoelectrophoretic analysis followed by Sepharose 4B-anti-human IgG affinity chromatography of the fraction having the inhibitory activity revealed the causative factor to be most likely IgG or a substance copurified with IgG. The results are discussed with respect to a possible role of IgG in a localized non-specific suppressor mechanism in the decidua preventing maternal immunologic attack on the fetal allograft.

Adult↗
PubMed1989

Isolation and characterization of an Escherichia coli mutant having temperature-sensitive farnesyl diphosphate synthase.

The screening of a collection of highly mutagenized strains of Escherichia coli for defects in isoprenoid synthesis led to the isolation of a mutant that had temperature-sensitive farnesyl diphosphate synthase. The defective gene, named ispA, was mapped at about min 10 on the E. coli chromosome, and the gene order was shown to be tsx-ispA-lon. The mutant ispA gene was transferred to the E. coli strain with a defined genetic background by P1 transduction for investigation of its function. The in vitro activity of farnesyl diphosphate synthase of the mutant was 21% of that of the wild-type strain at 30 degrees C and 5% of that at 40 degrees C. At 42 degrees C the ubiquinone level was lower (66% of normal) in the mutant than in the wild-type strain, whereas at 30 degrees C the level in the mutant was almost equal to that in the wild-type strain. The polyprenyl phosphate level was slightly higher in the mutant than in the wild-type strain at 30 degrees C and almost the same in both strains at 42 degrees C. The mutant had no obvious phenotype regarding its growth properties.

Alkyl and Aryl Transferases↗
PubMed1988

Acute glomerulonephritis during the third trimester of pregnancy.

We report a very rare case of acute poststreptococcal glomerulonephritis (PSGN) occurring in the eighth gestational month. The pregnancy succeeded despite initially severe nephrotic syndrome, and after delivery there was a complete recovery to normal renal function. Fetal prognosis was also good. The diagnosis of PSGN was made based on serological examinations and postpartum renal biopsy. Light and electron microscopic findings showed that the glomerular lesions were different from those of typical pre-eclampsia.

Acute Disease↗
PubMed1988

Effects of pregnancy and hormone treatments on pressor response to angiotensin II in conscious rats.

Pressor responses to graded doses of angiotensin II in conscious rats were significantly reduced on days 13 and 19 of pregnancy compared with those in nonpregnant rats. To study the hormonal regulation of this altered pressor response to angiotensin II during pregnancy, we administered estradiol, progesterone, and human chorionic gonadotropin to nonpregnant rats. In ovariectomized rats no effect of estradiol on the pressor response to angiotensin II was found, but injections of progesterone with or without estradiol pretreatment significantly reduced the pressor response to angiotensin II. In intact rats human chorionic gonadotropin induced elevation of endogenous progesterone levels, followed by a significant decrease in the pressor response to angiotensin II. Injection of estradiol after human chorionic gonadotropin pretreatment produced a significant elevation in the pressor response to angiotensin II. These findings indicate that the decrease in angiotensin pressor response in pregnant rats is mediated mainly by progesterone rather than by estrogen.

Angiotensin II↗
PubMed1988

Adult T-cell leukemia/lymphoma in pregnancy.

Adult T-cell leukemia/lymphoma, a lymphocytic leukemia caused by human T-cell lymphoma virus-I (HTLV-I), is prevalent in southwestern Japan. A Japanese woman with adult T-cell leukemia/lymphoma in the third trimester of pregnancy was delivered of an infant by cesarean section. She did not breast-feed the child, who has remained free from HTLV-I infection.

Adult↗
PubMed1987

Uterine papillary serous carcinoma with high levels of serum carcinoembryonic antigen. Response to combination chemotherapy.

A case of metastatic uterine papillary carcinoma with elevated serum carcinoembryonic antigen (CEA) is reported. The patient presented with ascites and pleural effusions and with a high level of CEA (258 ng/ml) 3 months after primary surgical resection and postoperative irradiation had been performed. Her complete response, although temporary, to two kinds of combination chemotherapy was evaluated using serial estimations of the serum CEA.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1987

Theca lutein cysts with maternal virilization and elevated serum testosterone in pregnancy.

A clinicopathological study was performed on a pregnant patient with bilateral multiple ovarian cysts, who presented with pre-eclampsia, marked virilization and exceedingly high levels of serum testosterone. The female fetus showed no evidence of virilization. Microscopic examination of the ovaries revealed theca lutein cysts with granulosa cells which showed the existence of testosterone by immunoperoxidase staining.

Adult↗
PubMed1987

Scanning electron microscopic study on the angioarchitecture of glomeruli in experimental toxemia of pregnancy in the rabbit.

Experimental toxemia of pregnancy in late gestation in rabbits was induced by constricting the aorta below the renal arteries on day 21 of gestation. Light and transmission electron microscopic examination of glomeruli on day 29 revealed only a slight swelling of endothelial cells. The angioarchitecture was also investigated using scanning electron microscopic examination of methyl methacry corrosion casts of the glomeruli. The kidney in experimental toxemia was characterized by a reduction of glomerular vessel diameter compared to that of normal pregnant rabbits. This probably resulted from enlargement of the endothelial cells, suggesting a reduced blood supply to the glomerulus. These findings suggest that interference with the blood supply to the pregnant uterus is an important factor in the development of the renal lesion of experimental toxemia of pregnancy in the rabbit.

Animals↗