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Biomedical subjects

S Fujisaki

Publications and source records attributed to S Fujisaki.

At least 91 records · Page 5Linked to original sources
PubMed1986

Vasopressor activity in incubated plasma of normal and hypertensive pregnant women.

The pressor activity generated in incubated plasma of nonpregnant, normal pregnant, and hypertensive pregnant women was measured by means of a sensitive bioassay technique. It was found that the plasma of normal pregnant women generated significantly higher amounts of active pressor principle than the plasma of nonpregnant women. The plasma of hypertensive pregnant women generated significantly lower amounts of active pressor principle than the plasma of normal pregnant women. Plasma obtained from the antecubital vein revealed no difference in pressor activity compared to plasma collected from the uterine vein at the time of cesarean section. These data suggest that active pressor principle is not involved in the pathogenesis of pregnancy-induced hypertension and that the pregnant uterus is not the source of active pressor principle.

Angiotensin II↗
PubMed1986

Craniofacial defects associated with amniotic band syndrome: a case report.

A pathological study was made of a female infant with amniotic band syndrome. At birth, the 1450-g female infant was noted to have craniofacial defects such as anencephaly, cleft lip, severe nasal deformity and asymmetric microphthalmia. The fetal membrane was focally adhered to the scalp, and histological examinations revealed that the connecting area of the fetal head to the membrane resembled a serial architecture from keratinized squamous cells to amniotic layer without any inflammatory findings. These observations suggested that the etiology in the present case may be consistent with Torpin's mesodermic band theory.

Abnormalities, Multiple↗
PubMed1986

Fetal heart monitoring and ultrasound in the management of placental abruption.

Three cases of placental abruption with the "pseudosinusoidal" fetal heart rate (FHR) pattern, a periodic late deceleration related to frequent uterine contractions are reported. This 'pseudosinusoidal' pattern is clearly not a true sinusoidal pattern. The fetal monitoring patterns of these patients are presented and discussed along with the use of ultrasound in the management of this condition. All infants were delivered by cesarean section with low Apgar scores at birth, but they soon recovered completely.

Abruptio Placentae↗
PubMed1986

Effects of highly purified eicosapentaenoic acid on vascular reactivity to angiotensin II and norepinephrine in the rabbit.

There is disagreement about whether supplementation of the diet with fish oil, which is rich in eicosapentaenoic acid (EPA), lowers blood pressure. We gave highly purified EPA in a soft capsule (90% ethyl ester form of EPA; EPA-E), to female rabbits (100 mg/kg/day) for 4 weeks. Vascular response to vasoconstrictor agents was assessed serially by measuring the systolic blood pressure using a Grand-Rothschild capsule in the ear. There was no change in systolic blood pressure of rabbits treated with EPA-E, but rabbits given EPA-E for one week or longer were significantly less responsive to the pressor effects of angiotensin II than the controls. Responses to norepinephrine did not change. Rabbits given EPA-E for four weeks had significantly more EPA in the serum, but there were no differences in serum levels of triglycerides, total cholesterol, or high-density lipoprotein cholesterol. These results suggest that vascular responses to exogenous angiotensin II can be selectively depressed by short-term treatment with EPA-E in rabbits without changing systolic blood pressure.

Angiotensin II↗
PubMed1986

Small cell carcinoma of the endometrium associated with adenosquamous carcinoma: a light and electron microscopic study.

This report describes a 64-year-old woman with a primary small cell carcinoma of the endometrium associated with adenosquamous carcinoma. The light microscopic features resembled those of small cell carcinoma of the lung and those of the uterine cervix, and foci of adenosquamous carcinoma lay scattered sparsely in the small cell carcinoma. Electron microscopy revealed cytoplasmic neurosecretory type granules. The neoplasm behaved in a very aggressive manner such that at 3 months after surgery a metastatic neoplasm appeared in the vagina. This case is a rare example of an endometrial carcinoma with differentiation toward endocrine as well as adeno and squamous cell carcinoma.

Adenocarcinoma↗
PubMed1986

Biosynthesis of isoprenoids in intact cells of Escherichia coli.

Upon rehydration of lyophilized Escherichia coli cells with phosphate buffer containing [14C]isopentenyl pyrophosphate (IPP), 14C was incorporated into the cells. Radioactivity was found in ubiquinone-8, an unidentified precursor of ubiquinone-8, demethylmenaquinone-8 and phosphate esters of all-trans-octaprenol and cis, trans-polyprenols. On rehydration of the cells with the buffer containing geranyl pyrophosphate or farnesyl pyrophosphate in combination with [14C]IPP, higher radioactivity was incorporated into the above products and some radioactivity was found in free prenols. Fractionation of the 14C-labeled cells by sucrose-density gradient centrifugation before and after recultivation indicated that the size of 14C-labeled cells had changed during the recultivation. This shows that radioactivity of [14C]IPP was incorporated into live cells but not into dead cells. The metabolism of the radioactive products in the recultivated cells was examined. It was found that the unidentified precursor was converted to ubiquinone-8, but demethylmenaquinone-8 was not converted to menaquinone-8. "Lipid intermediates" in peptidoglycan synthesis increased in the logarithmic growth phase and decreased in the stationary phase. In the stationary phase, however, an increase in cis,trans-polyprenyl monophosphates was observed. These observations suggest the operation of the lipid cycle of peptidoglycan synthesis.

Carbon Radioisotopes↗
PubMed1986

Isoprenoid synthesis in Escherichia coli. Separation and partial purification of four enzymes involved in the synthesis.

Isopentenyl pyrophosphate (IPP) isomerase, farnesyl pyrophosphate (FPP) synthetase, octaprenyl pyrophosphate (OPP) synthetase and undecaprenyl pyrophosphate (UPP) synthetase were partially purified from Escherichia coli by DEAE-Toyopearl chromatography. FPP synthetase catalyzed the condensation of IPP with dimethylallyl pyrophosphate (DPP) as well as with geranyl pyrophosphate (GPP) to yield FPP as final product. OPP synthetase and UPP synthetase catalyzed the condensation of IPP with FPP to yield OPP and cis,trans-polyprenyl pyrophosphates (the C45-, C50, and C55-compound), respectively. Neither DPP nor GPP acted as a priming substrate for either enzyme. These four enzymes required Mg2+ or Mn2+ for their activities. UPP synthetase required also Triton X-100 for its activity. The addition of Triton X-100 enhanced OPP synthetase, but it did not affect IPP isomerase and FPP synthetase. It seems possible that the combination of the four enzymes ensures the in vivo synthesis of long-chain isoprenoids in E. coli.

Alkyl and Aryl Transferases↗
PubMed1986

Pseudomyxoma peritonei: effect of chronic continuous immunotherapy with a streptococcal preparation, OK-432 after surgery.

Three cases of pseudomyxoma peritonei with ovarian neoplasma were treated by adjunctive immunotherapy with OK-432 after surgery. In 2 of the 3 cases, the periods of intramuscular injection of OK-432 were 11 and 16 months, respectively, and the 2 patients remain clinically free from evidence of disease, 7 and 6 years after surgery. The third patient is still undergoing treatment, with no evidence of recurrence at 4 months after surgery. These results, although for a small number of patients, clearly suggest that adjunctive immunotherapy with OK-432 may be suitable for treating pseudomyxoma peritonei of ovarian tumor origin.

Aged↗
PubMed1986

Fetal blood velocity waveforms in uncomplicated pregnancy.

Umbilical artery velocity waves were measured in fetuses from 94 normal pregnant women. In all, 183 determinations were carried out from the 14th to the 40th week of gestation. A combination of pulsed echo and real-time scanning was used to obtain blood waveforms from the umbilical arteries. The umbilical artery velocity wave can be readily differentiated from other fetal signals by its pattern. The systolic peak of the velocity wave was divided by the end diastolic value, thereby giving an S/D ratio. The S/D ratio in normal pregnancy declined from 7.6 to 2.0 from 14 to 40 weeks. Analysis of these waveforms indicated that the placenta is an organ of low vascular resistance and that placental resistance to blood flow declines with advancing gestational age in normal pregnancy. The umbilical artery S/D ratio provides a new and non-invasive marker of fetoplacental blood flow resistance.

Blood Flow Velocity↗
PubMed1986

Immunological studies of human endometrium--suppression of lymphocyte mitogenesis by endometrial extract.

Immunoregulatory activity that suppresses lymphocyte mitogenesis was demonstrated in human secretory endometrial extracts (SEE). SEE markedly suppressed 3H-thymidine (3H-TdR) incorporation by phytohemagglutinin (PHA)-stimulated lymphocytes compared with myometrial extracts from the same donors. SEE-induced inhibition of lymphocyte response to PHA was significantly (p less than 0.01) greater than that in cultures containing proliferative endometrial extracts at equivalent concentrations. SEE inhibited PHA- and ConA-stimulated cultures much better than PWM cultures, suggesting that it suppressed mitogen stimulation of T cells, but probably not B cells. Investigation of the kinetics of SEE-mediated suppression revealed that it was effective in inhibiting the initial activation processes of T-cell mitogenesis. Preincubation of lymphocytes with SEE followed by washing prevented inhibition of PHA-stimulated proliferation, indicating that the inhibitory effect of SEE might be reversible. The results are discussed with respect to a possible role of the inhibitory factor(s) in SEE in facilitating the implantation process of fetal allograft.

Cells, Cultured↗
PubMed1986

Clear cell carcinoma and endometrioid carcinoma arising in a endometriotic cyst of the ovary.

A case of two tumors arising in an endometriotic cyst of the ovary is described. These two tumors are macroscopically polypous and are histologically a clear cell carcinoma and an endometrioid carcinoma, respectively. This is a rare neoplasm and its existence supports the general opinion that clear cell carcinoma and endometrioid carcinoma of the ovary are both müllerian in origin.

Adenocarcinoma↗
PubMed1986

A case of meconium peritonitis: the pathological findings.

An autopsy case of meconium peritonitis is described. This baby was born at 24 weeks of gestation. The pathological findings were generalized peritonitis and this inflammation spread to the umbilical cord. It can be thought that the cause of premature labor may be in part some inflammatory process.

Female↗
PubMed1985

Synergistic effect of progesterone on prostaglandin E modulation of the mitogenic response of human peripheral lymphocytes.

The combined effects of prostaglandins of the E series (PGE) and progesterone (P), both of which are found to increase locally in the secretory endometrium, were studied in mitogen-stimulated cultures of lymphocytes from human peripheral blood. When added separately to lymphocyte cultures, both PGE and P produced a concentration-dependent inhibition of T-cell, but not B-cell, mitogenesis. If added together, these agents caused a much greater inhibition of T-cell mitogenesis, with marked synergy, than that observed with either agent alone. The synergistic inhibition was achieved with endometrial concentrations of PGE and P. The kinetics of PGE- and P-mediated inhibition investigated at various times during lymphocyte activation indicated that both agents affected the early events of T-cell mitogenesis. PGE- and P-inhibition of T-cell mitogenesis were shown to be reversible phenomena by washing the cells treated with these agents. These results suggest that elevated levels of PGE and P in the secretory endometrium, acting synergistically in inhibiting the proliferative response of T cells, may participate in facilitating implantation of histoincompatible fetal tissue in the maternal uterus as non-specific local immunosuppressive factors.

Adult↗